Multiplexed integrating plasmids for engineering of the erythromycin gene cluster for expression in Streptomyces spp. and combinatorial biosynthesis

• Published in: Applied and Environmental Microbiology Vol. 81; no. 24; pp. 8402 - 8413
• Main Authors: Fayed, Bahgat, Ashford, David A, Hashem, Amal M, Amin, Magdy A, El Gazayerly, Omaima N, Gregory, Matthew A, Smith, Margaret C M
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.12.2015
• Subjects: Anti-Bacterial Agents - biosynthesis; Bacteria; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Biosynthesis; Biotechnology; Chromatography, Liquid; Erythromycin - analogs & derivatives; Erythromycin - biosynthesis; Erythromycin - metabolism; Genetic Engineering; Genomes; Glycosylation; Mass Spectrometry; Metabolites; Microbiology; Multienzyme Complexes - metabolism; Multigene Family - genetics; Plasmids; Plasmids - genetics; Streptomyces; Streptomyces - genetics; Streptomyces - metabolism

Bacteria in the genus Streptomyces and its close relatives are prolific producers of secondary metabolites with antibiotic activity. Genome sequencing of these bacteria has revealed a rich source of potentially new antibiotic pathways, whose products have never been observed. Moreover, these new pat...