Enhanced 5-Aminolevulinic Acid Production by Co-expression of Codon-Optimized hemA Gene with Chaperone in Genetic Engineered Escherichia coli

• Published in: Applied biochemistry and biotechnology Vol. 191; no. 1; pp. 299 - 312
• Main Authors: Yu, Tzu-Hsuan, Yi, Ying-Chen, Shih, I-Tai, Ng, I-Son
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2020; Springer Nature B.V
• Subjects: Acid production; Aldehyde Oxidoreductases - biosynthesis; Aldehyde Oxidoreductases - genetics; Aminolevulinic acid; Aminolevulinic Acid - metabolism; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Biochemistry; Biotechnology; C4 photosynthesis; Chemistry; Chemistry and Materials Science; Codon; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Fermentation; Gene expression; genes; Glycine; HemA gene; medicine; Microorganisms, Genetically-Modified - genetics; Microorganisms, Genetically-Modified - metabolism; Optimization; pyridoxal; Rhodobacter capsulatus; Rhodobacter capsulatus - enzymology; Rhodobacter capsulatus - genetics; Substrates; succinic acid; Transcription; transcription (genetics); 5-aminolevulinic acid; Chaperone; Rhodobacter capsulatus; hemA; Escherichia coli

5-Aminolevulinic acid (ALA) is an important metabolic intermediate compound with high value and has recently been used in agriculture and medicine. In this study, we have constructed six recombinant Escherichia coli ( E. coli ) strains that are involved in pET system under the regulation of the T7 p...