Characterization of a Nitrilase and a Nitrile Hydratase from Pseudomonas sp. Strain UW4 That Converts Indole-3-Acetonitrile to Indole-3-Acetic Acid

• Published in: Applied and environmental microbiology Vol. 80; no. 15; pp. 4640 - 4649
• Main Authors: Duca, Daiana, Rose, David R., Glick, Bernard R.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.08.2014
• Subjects: Acetic acid; Acids; Amino Acid Sequence; Aminohydrolases - chemistry; Aminohydrolases - genetics; Aminohydrolases - metabolism; Bacteria; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biosynthesis; Biotransformation; E coli; Enzyme Stability; Enzymes; Escherichia coli; Hydro-Lyases - chemistry; Hydro-Lyases - genetics; Hydro-Lyases - metabolism; Hydrogen-Ion Concentration; Indoleacetic Acids - metabolism; Indoles - metabolism; Molecular Sequence Data; Physicochemical properties; Plant growth; Plant Microbiology; Pseudomonas; Pseudomonas - chemistry; Pseudomonas - enzymology; Pseudomonas - genetics; Pseudomonas - metabolism; Sequence Alignment; Substrate Specificity; Temperature

Indole-3-acetic acid (IAA) is a fundamental phytohormone with the ability to control many aspects of plant growth and development. Pseudomonas sp. strain UW4 is a rhizospheric plant growth-promoting bacterium that produces and secretes IAA. While several putative IAA biosynthetic genes have been rep...