A Rapid PCR Method of Genotyping LDL Receptor Mutations in WHHL Rabbits

WHHL rabbits are a valuable model for the study of human familial hypercholesterolemia and atherosclerosis. To use this animal model, it is often necessary to confirm LDL receptor status in WHHL rabbits. Here, we described a simple and rapid PCR method to detect LDL mutations in WHHL rabbits.

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Bibliographic Details
Published inJournal of Atherosclerosis and Thrombosis Vol. 9; no. 3; pp. 145 - 148
Main Authors Sun, Huijun, Usui, Shin-ichi, Shiomi, Masashi, Watanabe, Teruo, Fan, Jianglin
Format Journal Article
LanguageEnglish
Published Japan Japan Atherosclerosis Society 2002
Subjects
Animals
Atherosclerosis
Base Sequence
Disease Models, Animal
DNA Primers
Genotype
Hypercholesterolemia
Hyperlipoproteinemia Type II - genetics
LDL receptor
Mutation
PCR
Polymerase Chain Reaction - methods
Rabbits
Receptors, LDL - genetics
Transgenic rabbit
WHHL
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Summary:WHHL rabbits are a valuable model for the study of human familial hypercholesterolemia and atherosclerosis. To use this animal model, it is often necessary to confirm LDL receptor status in WHHL rabbits. Here, we described a simple and rapid PCR method to detect LDL mutations in WHHL rabbits.
ISSN:1340-3478
1880-3873
DOI:10.5551/jat.9.145