Mass Spectrometric Method for Analyzing Metabolites in Yeast with Single Cell Sensitivity

Getting a look‐in: An optimized MALDI‐MS procedure has been developed to detect endogenous primary metabolites directly in the cell extract. A detection limit corresponding to metabolites from less than a single cell has been attained, opening the door to single‐cell metabolomics by mass spectrometr...

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Published inAngewandte Chemie (International ed.) Vol. 47; no. 29; pp. 5382 - 5385
Main Authors Amantonico, Andrea, Oh, Joo Yeon, Sobek, Jens, Heinemann, Matthias, Zenobi, Renato
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 07.07.2008
WILEY-VCH Verlag
WILEY‐VCH Verlag
Subjects
Acetyl Coenzyme A - analysis
Adenosine Diphosphate - analysis
Adenosine Triphosphate - analysis
Cytological Techniques
Guanosine Diphosphate - analysis
Guanosine Triphosphate - analysis
high-throughput screening
mass spectrometry
Metabolism
metabolites
microscale preparation
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - metabolism
single-cell analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Getting a look‐in: An optimized MALDI‐MS procedure has been developed to detect endogenous primary metabolites directly in the cell extract. A detection limit corresponding to metabolites from less than a single cell has been attained, opening the door to single‐cell metabolomics by mass spectrometry.
Bibliography:http://dx.doi.org/10.1002/anie.200705923
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ark:/67375/WNG-J39S0VMX-S
Korean Government - No. KRF-2006-214-C00062
We thank Anne Kümmel and Jennifer Ewald for the yeast cultivation, sample quenching, and extraction, and Benjamin Volkmer for the flow cytometric analysis. We also thank Ruth Hüttenhain for the MS/MS experiments. This work was supported by the ETH-INIT "Single cell metabolomics" project. Dr. Oh was supported by a Korean Research Foundation Grant from the Korean Government (MOEHRD, Basic Research Promotion Fund, grant no. KRF-2006-214-C00062).
ArticleID:ANIE200705923
We thank Anne Kümmel and Jennifer Ewald for the yeast cultivation, sample quenching, and extraction, and Benjamin Volkmer for the flow cytometric analysis. We also thank Ruth Hüttenhain for the MS/MS experiments. This work was supported by the ETH‐INIT “Single cell metabolomics” project. Dr. Oh was supported by a Korean Research Foundation Grant from the Korean Government (MOEHRD, Basic Research Promotion Fund, grant no. KRF‐2006‐214‐C00062).
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content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200705923