EYA1 and SIX1 drive the neuronal developmental program in cooperation with the SWI/SNF chromatin-remodeling complex and SOX2 in the mammalian inner ear

• Published in: Development (Cambridge) Vol. 139; no. 11; pp. 1965 - 1977
• Main Authors: Ahmed, Mohi, Xu, Jinshu, Xu, Pin-Xian
• Format: Journal Article
• Language: English
• Published: England Company of Biologists 01.06.2012
• Subjects: Adenosinetriphosphatase; Animals; Basic Helix-Loop-Helix Transcription Factors - metabolism; Beta2 protein; BRG1 protein; Chromatin remodeling; Cochlea; Differentiation; DNA Primers - genetics; Ear, Inner - embryology; Ear, Inner - innervation; Electroporation; Epithelial cells; Fibroblasts; Galactosides; Gene Expression Regulation, Developmental - physiology; Gene regulation; Helix-loop-helix proteins; Histological Techniques; Homeodomain Proteins - metabolism; Immunohistochemistry; In Situ Hybridization; Indoles; Inner ear; Intracellular Signaling Peptides and Proteins - metabolism; Mice; Mice, Inbred Strains; Nerve Tissue Proteins - metabolism; Neurofilaments; Neurogenesis; Neurogenesis - genetics; Neurogenesis - physiology; NIH 3T3 Cells; Nuclear Proteins - metabolism; otocysts; Protein Tyrosine Phosphatases - metabolism; Real-Time Polymerase Chain Reaction; SOXB1 Transcription Factors - metabolism; Transcription factors; Two-Hybrid System Techniques

Inner ear neurogenesis depends upon the function of the proneural basic helix-loop-helix (bHLH) transcription factors NEUROG1 and NEUROD1. However, the transcriptional regulation of these factors is unknown. Here, using loss- and gain-of-function models, we show that EYA1 and SIX1 are crucial otic n...