Good correlation between RT-PCR analysis and relapse in Philadelphia (Ph1)-positive acute lymphoblastic leukemia (ALL)

• Published in: Leukemia Vol. 11; no. 2; pp. 294 - 298
• Main Authors: PREUDHOMME, C, HENIC, N, COSSON, A, FENAUX, P, CAZIN, B, LAÏ, J. L, BERTHEAS, M. F, VANRUMBEKE, M, LEMOINE, F, JOUET, J. P, DECONNINCK, E, NELKEN, B
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 01.02.1997; Nature Publishing Group
• Subjects: Abl protein; Acute lymphoblastic leukemia; Adult; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; BCR protein; Biological and medical sciences; Bone marrow; Bone Marrow - pathology; Bone Marrow Transplantation; Chemotherapy; Child; Cytogenetics; DNA, Neoplasm - genetics; Follow-Up Studies; Fusion protein; Fusion Proteins, bcr-abl - genetics; Hematology; Humans; Investigative techniques, diagnostic techniques (general aspects); Leukemia; Lymphatic leukemia; Medical sciences; Neoplasm Proteins - genetics; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Polymerase Chain Reaction; Precursor Cell Lymphoblastic Leukemia-Lymphoma - blood; Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor Cell Lymphoblastic Leukemia-Lymphoma - mortality; Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology; Precursor Cell Lymphoblastic Leukemia-Lymphoma - therapy; Predictive Value of Tests; Prognosis; Prospective Studies; Recurrence; Remission; RNA, Messenger - analysis; RNA, Messenger - blood; RNA, Neoplasm - analysis; RNA, Neoplasm - blood; Sensitivity analysis; Sensitivity and Specificity; Treatment Failure; Chromosomal aberration; Human; Relapse; RNA-directed DNA polymerase; Enzyme; Transferases; Acute; Minimal residual disease; Malignant hemopathy; Philadelphia chromosome; Polymerase chain reaction; Nucleotidyltransferases; Lymphoproliferative syndrome; C-Onc gene; Cytogenetics; Abnormal chromosome; Diagnosis; Acute lymphocytic leukemia; Molecular biology; Hybrid gene; Protooncogene; Chromosome translocation

We sequentially performed cytogenetic analysis and RT-PCR analysis of BCR-ABL transcripts in 17 cases of Ph1-positive ALL who had achieved hematological complete remission (CR) with intensive chemotherapy (CT). Sixteen cases were studied prospectively. All but one of the patients had reached cytogen...