Evaluation of a shortened QIAsymphony DNA extraction protocol for stool samples using a multiplex real-time PCR for the detection of enteric pathogens

A shortened DNA extraction protocol for the QIAsymphony SP was evaluated by quantitative and qualitative comparison of real-time PCR results of 150 co-extracted stool samples. The average ∆Cycle threshold value for positive pathogenic targets was 0.28 Ct. A consensus of 96.91%, with a correlation co...

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Published inJournal of microbiological methods Vol. 84; no. 2; pp. 365 - 367
Main Authors van Zanten, E., Wisselink, G.J., Stoll, S., Alvarez, R., Kooistra-Smid, A.M.D.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.02.2011
Elsevier
Subjects
Bacteriological Techniques - methods
Biological and medical sciences
correlation
DNA
DNA - isolation & purification
DNA extraction
Enteric pathogens
Enterobacteriaceae - genetics
Enterobacteriaceae - isolation & purification
Evaluation
feces
Feces - microbiology
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
pathogens
QIAsymphony
quantitative polymerase chain reaction
Real-time PCR
Time Factors
Evaluation
DNA extraction
Real-time PCR
QIAsymphony
Enteric pathogens
Polymerase chain reaction
Multiplex polymerase chain reaction
Feces
Detection
Real time
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Summary:A shortened DNA extraction protocol for the QIAsymphony SP was evaluated by quantitative and qualitative comparison of real-time PCR results of 150 co-extracted stool samples. The average ∆Cycle threshold value for positive pathogenic targets was 0.28 Ct. A consensus of 96.91%, with a correlation coefficient of 0.9880 was recorded.
Bibliography:http://dx.doi.org/10.1016/j.mimet.2010.12.023
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ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2010.12.023