Immunocapture and Microplate-Based Activity and Quantity Measurement of Pyruvate Dehydrogenase in Human Peripheral Blood Mononuclear Cells

• Published in: Bioanalysis Vol. 7; no. 5; pp. 583 - 592
• Main Authors: Liu, Xiaowen, Pervez, Hira, Andersen, Lars W, Uber, Amy, Montissol, Sophia, Patel, Parth, Donnino, Michael W
• Format: Journal Article
• Language: English
• Published: England 01.03.2015
• Subjects: Humans; Leukocytes, Mononuclear - immunology; Pyruvate Dehydrogenase Complex - immunology

Pyruvate dehydrogenase (PDH) activity is altered in many human disorders. Current methods require tissue samples and yield inconsistent results. We describe a modified method for measuring PDH activity from isolated human peripheral blood mononuclear cells (PBMCs). RESULTS/METHODOLOGY: We found that...