Real‐Time Intracellular Measurements of ROS and RNS in Living Cells with Single Core–Shell Nanowire Electrodes

• Published in: Angewandte Chemie International Edition Vol. 56; no. 42; pp. 12997 - 13000
• Main Authors: Zhang, Xin‐Wei, Qiu, Quan‐Fa, Jiang, Hong, Zhang, Fu‐Li, Liu, Yan‐Lin, Amatore, Christian, Huang, Wei‐Hua
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 09.10.2017; Wiley-VCH Verlag
• Edition: International ed. in English
• Subjects: amperometry; Analytical chemistry; Animals; Biological activity; Biomonitoring; Carbon Compounds, Inorganic - chemistry; Chemical Sciences; Cylindrical shells; Electrochemical analysis; Electrochemical Techniques; Electrochemistry; Electrodes; HeLa Cells; Humans; Intracellular; Macrophages; Macrophages - cytology; Macrophages - metabolism; Mice; Microscopy, Fluorescence; nanoelectrodes; Nanotechnology; Nanowires; Nanowires - chemistry; Organelles; Phagolysosomes; Phagosomes - metabolism; RAW 264.7 Cells; reactive nitrogen species; Reactive Nitrogen Species - analysis; Reactive Nitrogen Species - metabolism; reactive oxygen species; Reactive Oxygen Species - analysis; Reactive Oxygen Species - metabolism; Real time; Robustness (mathematics); Silicon carbide; Silicon Compounds - chemistry; amperometry; reactive nitrogen species; reactive oxygen species; nanoelectrodes; phagolysosomes

Nanoelectrodes allow precise and quantitative measurements of important biological processes at the single living‐cell level in real time. Cylindrical nanowire electrodes (NWEs) required for intracellular measurements create a great challenge for achieving excellent electrochemical and mechanical pe...