Multiplex identification of drug-resistant Gram-positive pathogens using stuffer-free MLPA system

• Published in: Electrophoresis Vol. 37; no. 23-24; pp. 3079 - 3083
• Main Authors: Chung, Boram, Park, Chulmin, Cho, Sung-Yeon, Shin, Sun, Yim, Seon-Hee, Jung, Gyoo Yeol, Lee, Dong-Gun, Chung, Yeun-Jun
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.12.2016
• Subjects: Blood; Capillary electrophoresis; Decisions; Drugs; Electrophoresis; Electrophoresis, Capillary - methods; Enterococcus faecium; Genes; Gram-Positive Bacteria - genetics; Gram-Positive Bacterial Infections - microbiology; Gram-positive pathogen; Humans; Limit of Detection; Multiplex ligation-dependent probe amplification; Multiplex Polymerase Chain Reaction - methods; Multiplexing; Pathogens; Reproducibility of Results; Sensitivity; Sepsis; Sepsis - microbiology; Staphylococcus aureus; Streptococcus pneumoniae; Sepsis; Gram-positive pathogen; Multiplex ligation-dependent probe amplification; Capillary electrophoresis

Early detection of pathogens from blood and identification of their drug resistance are essential for sepsis management. However, conventional culture‐based methods require relatively longer time to identify drug‐resistant pathogens, which delays therapeutic decisions. For precise multiplex detectio...