Monosynaptic restriction of the anterograde herpes simplex virus strain H129 for neural circuit tracing

• Published in: Journal of comparative neurology (1911) Vol. 531; no. 5; pp. 584 - 595
• Main Authors: Fischer, Kyle B., Collins, Hannah K., Pang, Yan, Roy, Dheeraj S., Zhang, Ying, Feng, Guoping, Li, Shu‐Jing, Kepecs, Adam, Callaway, Edward M.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2023
• Subjects: Brain; Gene deletion; Genomes; Herpes simplex; Herpesvirus 1, Human - genetics; Nervous system; Neural networks; Neurons; Packaging; Viruses

Identification of synaptic partners is a fundamental task for systems neuroscience. To date, few reliable techniques exist for whole brain labeling of downstream synaptic partners in a cell‐type‐dependent and monosynaptic manner. Herein, we describe a novel monosynaptic anterograde tracing system ba...