Impaired motor coordination in mice lacking the EDA exon of the fibronectin gene
The extracellular matrix (ECM) plays an important role in the central nervous system (CNS) by modulating the migration of cells, axons and dendrites of neurons. Fibronectin (FN) is a major component of the ECM in the CNS and plays essential roles in development, cell adhesion and cell migration. Spe...
Saved in:
Published in | Behavioural brain research Vol. 161; no. 1; pp. 31 - 38 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier B.V
03.06.2005
Elsevier Science |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The extracellular matrix (ECM) plays an important role in the central nervous system (CNS) by modulating the migration of cells, axons and dendrites of neurons. Fibronectin (FN) is a major component of the ECM in the CNS and plays essential roles in development, cell adhesion and cell migration. Specific FN-isoforms, generated by alternative splicing at three conserved regions, the extra domain B (EDB), extra domain A (EDA) and type III homologies connecting segment (IIICS), have been shown to modulate these processes in vitro and in vivo. The inclusion of the EDA exon in the brain is highly regulated during development and aging, suggesting an important role of this exon in brain function. However, the direct role of FN-isoforms in brain function and behaviour is still obscure. Therefore, to directly assess the role of the FN-EDA exon in vivo, we have generated two mouse strains devoid of EDA exon regulated splicing in the FN gene that constitutively include (EDA
+/+) or exclude (EDA
−/−) the EDA exon in all tissues. Here, we show the behavioural consequences of the absence of regulated splicing of the EDA exon in the FN gene. Deletion of the EDA domain in the FN protein results in reduced motor-coordination abilities and vertical exploratory capacity, whereas mice that constitutively include the EDA domain displayed a decrease in locomotory activity in the open field (OF) test. These results strongly suggest that regulated splicing of the EDA exon is necessary for a normal function of the brain. |
---|---|
AbstractList | The extracellular matrix (ECM) plays an important role in the central nervous system (CNS) by modulating the migration of cells, axons and dendrites of neurons. Fibronectin (FN) is a major component of the ECM in the CNS and plays essential roles in development, cell adhesion and cell migration. Specific FN-isoforms, generated by alternative splicing at three conserved regions, the extra domain B (EDB), extra domain A (EDA) and type III homologies connecting segment (IIICS), have been shown to modulate these processes in vitro and in vivo. The inclusion of the EDA exon in the brain is highly regulated during development and aging, suggesting an important role of this exon in brain function. However, the direct role of FN-isoforms in brain function and behaviour is still obscure. Therefore, to directly assess the role of the FN-EDA exon in vivo, we have generated two mouse strains devoid of EDA exon regulated splicing in the FN gene that constitutively include (EDA
+/+) or exclude (EDA
−/−) the EDA exon in all tissues. Here, we show the behavioural consequences of the absence of regulated splicing of the EDA exon in the FN gene. Deletion of the EDA domain in the FN protein results in reduced motor-coordination abilities and vertical exploratory capacity, whereas mice that constitutively include the EDA domain displayed a decrease in locomotory activity in the open field (OF) test. These results strongly suggest that regulated splicing of the EDA exon is necessary for a normal function of the brain. The extracellular matrix (ECM) plays an important role in the central nervous system (CNS) by modulating the migration of cells, axons and dendrites of neurons. Fibronectin (FN) is a major component of the ECM in the CNS and plays essential roles in development, cell adhesion and cell migration. Specific FN-isoforms, generated by alternative splicing at three conserved regions, the extra domain B (EDB), extra domain A (EDA) and type III homologies connecting segment (IIICS), have been shown to modulate these processes in vitro and in vivo. The inclusion of the EDA exon in the brain is highly regulated during development and aging, suggesting an important role of this exon in brain function. However, the direct role of FN-isoforms in brain function and behaviour is still obscure. Therefore, to directly assess the role of the FN-EDA exon in vivo, we have generated two mouse strains devoid of EDA exon regulated splicing in the FN gene that constitutively include (EDA(+/+)) or exclude (EDA(-/-)) the EDA exon in all tissues. Here, we show the behavioural consequences of the absence of regulated splicing of the EDA exon in the FN gene. Deletion of the EDA domain in the FN protein results in reduced motor-coordination abilities and vertical exploratory capacity, whereas mice that constitutively include the EDA domain displayed a decrease in locomotory activity in the open field (OF) test. These results strongly suggest that regulated splicing of the EDA exon is necessary for a normal function of the brain. The extracellular matrix (ECM) plays an important role in the central nervous system (CNS) by modulating the migration of cells, axons and dendrites of neurons. Fibronectin (FN) is a major component of the ECM in the CNS and plays essential roles in development, cell adhesion and cell migration. Specific FN-isoforms, generated by alternative splicing at three conserved regions, the extra domain B (EDB), extra domain A (EDA) and type III homologies connecting segment (IIICS), have been shown to modulate these processes in vitro and in vivo. The inclusion of the EDA exon in the brain is highly regulated during development and aging, suggesting an important role of this exon in brain function. However, the direct role of FN-isoforms in brain function and behaviour is still obscure. Therefore, to directly assess the role of the FN-EDA exon in vivo, we have generated two mouse strains devoid of EDA exon regulated splicing in the FN gene that constitutively include (EDA super(+) super(/) super(+)) or exclude (EDA super(-) super(/) super(-)) the EDA exon in all tissues. Here, we show the behavioural consequences of the absence of regulated splicing of the EDA exon in the FN gene. Deletion of the EDA domain in the FN protein results in reduced motor-coordination abilities and vertical exploratory capacity, whereas mice that constitutively include the EDA domain displayed a decrease in locomotory activity in the open field (OF) test. These results strongly suggest that regulated splicing of the EDA exon is necessary for a normal function of the brain. n of the brain. |
Author | Chauhan, Anil Kumar Moretti, Federico Andrea Iaconcig, Alessandra Baralle, Francisco Ernesto Muro, Andrés Fernando |
Author_xml | – sequence: 1 givenname: Anil Kumar surname: Chauhan fullname: Chauhan, Anil Kumar email: anil@icgeb.org, anil@cbr.med.harvard.edu – sequence: 2 givenname: Federico Andrea surname: Moretti fullname: Moretti, Federico Andrea email: moretti@icgeb.org – sequence: 3 givenname: Alessandra surname: Iaconcig fullname: Iaconcig, Alessandra email: iaconcig@icgeb.org – sequence: 4 givenname: Francisco Ernesto surname: Baralle fullname: Baralle, Francisco Ernesto email: baralle@icgeb.org – sequence: 5 givenname: Andrés Fernando surname: Muro fullname: Muro, Andrés Fernando email: muro@icgeb.org |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16784824$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/15904707$$D View this record in MEDLINE/PubMed |
BookMark | eNqNkc9vFCEUgImpsdvqH-DFzMXeZn0wDDDx1NSqTZrYQz0TBh6VdQZWmDX2v5d1N-lNTV4ghO_9gO-MnMQUkZDXFNYUqHi3WY9jXjOAfg2sBjwjK6oka2XPhxOyqoxoecfUKTkrZQMAHHr6gpzSfgAuQa7I3c28NSGja-a0pNzYlLIL0SwhxSbEZg4Wm8nY7yE-NMs3bK4_XDb4q14m_-fsw5jrVHap8ANGfEmeezMVfHXcz8nXj9f3V5_b2y-fbq4ub1vLebe0Hihwr6ij0omeSe4leGGY9dhZ56Rlo-RIkY--rpQqQY1QzjHv1Gho352Ti0PdbU4_dlgWPYdicZpMxLQrWkjFexjYP0E69JwJ9R-g7IeuE7yC9ADanErJ6PU2h9nkR01B78Xoja5i9F6MBlYDas6bY_HdOKN7yjiaqMDbI2CKNZPPJtpQnrj9exTbN39_4LB-7s-AWRcbMFp01aJdtEvhL2P8BsQOq_I |
CODEN | BBREDI |
CitedBy_id | crossref_primary_10_1261_rna_554607 crossref_primary_10_1016_j_neubiorev_2017_04_003 crossref_primary_10_1002_jcp_31048 crossref_primary_10_1016_j_bbi_2018_02_011 crossref_primary_10_1002_path_2388 crossref_primary_10_1128_MCB_06327_11 crossref_primary_10_1016_j_atherosclerosis_2007_08_015 crossref_primary_10_1016_j_addr_2015_11_014 crossref_primary_10_1016_j_jmb_2011_05_031 crossref_primary_10_1016_j_molcel_2005_06_035 crossref_primary_10_1016_j_bbrc_2009_08_073 crossref_primary_10_1111_j_1601_183X_2009_00537_x crossref_primary_10_1016_j_biocel_2007_04_004 crossref_primary_10_3390_ijms23158418 crossref_primary_10_1007_s00018_016_2225_y crossref_primary_10_1371_journal_pone_0055384 crossref_primary_10_3389_fonc_2020_00641 crossref_primary_10_1096_fj_11_195461 |
Cites_doi | 10.1038/scientificamerican0686-42 10.1016/j.gene.2003.09.026 10.1016/S0166-4328(03)00109-8 10.1038/360361a0 10.1242/dev.106.2.375 10.1073/pnas.80.11.3218 10.1016/0003-2697(87)90021-2 10.1002/j.1460-2075.1984.tb01787.x 10.1083/jcb.113.5.1223 10.1128/MCB.24.3.1387-1400.2004 10.1101/gad.6.10.1821 10.1073/pnas.80.1.137 10.1016/0092-8674(85)90322-8 10.1126/science.1058040 10.1523/JNEUROSCI.05-08-02205.1985 10.1016/S0014-5793(98)01201-0 10.1083/jcb.200212079 10.1002/j.1460-2075.1987.tb02509.x 10.1002/j.1460-2075.1987.tb02547.x 10.1006/bbrc.1996.0561 10.1096/fasebj.10.2.8641558 10.1038/85471 10.1016/0167-4781(93)90178-G 10.1590/S0100-879X1999000500017 10.1242/dev.119.4.1079 10.1016/0092-8674(83)90175-7 10.1016/S0143-4179(98)90072-5 10.1016/0092-8674(90)90715-Q 10.3109/15419069609010767 10.1002/(SICI)1097-0177(200006)218:2<235::AID-DVDY2>3.0.CO;2-G 10.1242/jcs.112.22.3847 10.1006/excr.1995.1374 10.1038/266730a0 10.1083/jcb.96.2.462 10.1007/978-1-4612-3264-3 |
ContentType | Journal Article |
Copyright | 2005 Elsevier B.V. 2005 INIST-CNRS |
Copyright_xml | – notice: 2005 Elsevier B.V. – notice: 2005 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QG 7TK 8FD FR3 P64 RC3 7X8 |
DOI | 10.1016/j.bbr.2005.02.020 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Animal Behavior Abstracts Neurosciences Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Neurosciences Abstracts Animal Behavior Abstracts Genetics Abstracts Engineering Research Database Technology Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
DatabaseTitleList | MEDLINE Genetics Abstracts MEDLINE - Academic Neurosciences Abstracts |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology |
EISSN | 1872-7549 |
EndPage | 38 |
ExternalDocumentID | 10_1016_j_bbr_2005_02_020 15904707 16784824 S0166432805000689 |
Genre | Research Support, Non-U.S. Gov't Journal Article Comparative Study |
GroupedDBID | --- --K --M .GJ .~1 0R~ 1B1 1RT 1~. 1~5 23N 4.4 41~ 457 4G. 53G 5GY 5VS 6J9 7-5 71M 8P~ 9JM AABNK AACTN AADPK AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXLA AAXUO ABCQJ ABFNM ABFRF ABIVO ABJNI ABMAC ABTAH ABXDB ABYKQ ACDAQ ACGFO ACGFS ACIUM ACRLP ADBBV ADEZE ADIYS ADMUD AEBSH AEFWE AEKER AENEX AFKWA AFTJW AFXIZ AGHFR AGUBO AGWIK AGYEJ AHHHB AI. AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC C45 CS3 DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HMQ HVGLF HZ~ IHE J1W KOM L7B M2V M41 MO0 MOBAO N9A O-L O9- OAUVE OVD OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SCC SDF SDG SDP SES SEW SNS SPCBC SSN SSZ T5K TEORI TN5 VH1 WH7 WUQ XJT YR2 ZGI ZXP ZY4 ~G- AAPBV ABPIF ABPTK IQODW AKRWK CGR CUY CVF ECM EIF NPM AAXKI AAYXX CITATION 7QG 7TK 8FD FR3 P64 RC3 7X8 |
ID | FETCH-LOGICAL-c443t-f0104f81d17d65274f70f6a2cfe3cdd7c2b74e1e4bfe1e11861a68dd2fd8ba153 |
IEDL.DBID | AIKHN |
ISSN | 0166-4328 |
IngestDate | Fri Aug 16 23:17:28 EDT 2024 Sat Aug 17 02:46:10 EDT 2024 Fri Aug 16 04:23:04 EDT 2024 Thu Sep 12 19:50:59 EDT 2024 Thu May 23 23:11:03 EDT 2024 Sun Oct 22 16:04:04 EDT 2023 Fri Feb 23 02:25:18 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | Rotarod test Alternative splicing Extracellular matrix Motor coordination and balance Fibronectin isoforms Molecular form Targeting Rodentia Transgenic animal Motor control Open field behavior Muscular coordination Fibronectin Mus domesticus Vertebrata Mammalia Gene Motricity |
Language | English |
License | CC BY 4.0 |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c443t-f0104f81d17d65274f70f6a2cfe3cdd7c2b74e1e4bfe1e11861a68dd2fd8ba153 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
PMID | 15904707 |
PQID | 17593364 |
PQPubID | 23462 |
PageCount | 8 |
ParticipantIDs | proquest_miscellaneous_67845092 proquest_miscellaneous_19542682 proquest_miscellaneous_17593364 crossref_primary_10_1016_j_bbr_2005_02_020 pubmed_primary_15904707 pascalfrancis_primary_16784824 elsevier_sciencedirect_doi_10_1016_j_bbr_2005_02_020 |
PublicationCentury | 2000 |
PublicationDate | 2005-06-03 |
PublicationDateYYYYMMDD | 2005-06-03 |
PublicationDate_xml | – month: 06 year: 2005 text: 2005-06-03 day: 03 |
PublicationDecade | 2000 |
PublicationPlace | Shannon |
PublicationPlace_xml | – name: Shannon – name: Netherlands |
PublicationTitle | Behavioural brain research |
PublicationTitleAlternate | Behav Brain Res |
PublicationYear | 2005 |
Publisher | Elsevier B.V Elsevier Science |
Publisher_xml | – name: Elsevier B.V – name: Elsevier Science |
References | Freitag, Schachner, Morellini (bib7) 2003; 145 Komblihtt, Vibe-Pedersen, Baralle (bib17) 1984; 3 Pires Neto, Braga-de-Souza, Lent (bib26) 1999; 32 Guan, Hynes (bib11) 1990; 60 Mackie, Tucker (bib19) 1999; 112 Jones, Jones (bib14) 2000; 218 Border, Noble, Yamamoto, Harper, Yamaguchi, Pierschbacher (bib1) 1992; 360 Rovasio, Delouvee, Yamada, Timpl, Thiery (bib30) 1983; 96 Buratti, Muro, Giombi, Gherbassi, Iaconcig, Baralle (bib2) 2004; 24 Peters, Chen, Hynes (bib24) 1996; 4 Porsolt, Le Pichon, Jalfre (bib27) 1977; 266 Schwarzbauer, Patel, Fonda, Hynes (bib33) 1987; 6 Thiery, Duband, Dufour, Savagner, Imhof (bib35) 1989 Sakai (bib32) 2001; 7 Venter (bib36) 2001; 291 Saga, Yagi, Ikawa, Sakakura, Aizawa (bib31) 1992; 6 Zardi, Carnemolla, Siri, Petersen, Paolella, Sebastio (bib37) 1987; 6 Pagani, Zagato, Vergani, Casari, Sidoli, Baralle (bib23) 1991; 113 Komblihtt, Vibe-Pedersen, Baralle (bib16) 1983; 80 Hynes RO. Springer series in molecular biology. In: Rich A, editor. Fibronectins. New York: Springer–Verlag; 1990. Pytela, Pierschbacher, Ruoslahti (bib29) 1985; 40 Komblihtt, Pesce, Alonso, Cramer, Srebrow, Werbajh (bib15) 1996; 10 Chomczynski, Sacchi (bib4) 1987; 162 Liao, Gotwals, Koteliansky, Sheppard, Van De Water (bib18) 2002; 11 Pagani, Zagato, Maier, Ragnotti, Coviello, Vergani (bib22) 1993; 1173 Muro, Chauhan, Gajovic, Iaconcig, Porro, Stanta (bib20) 2003; 162 Fukamauchi, Aihara, Kusakabe (bib8) 1998; 32 Hynes (bib12) 1986; 254 Schwarzbauer, Tamkun, Lemischka, Hynes (bib34) 1983; 35 Petersen, Thogersen, Skorstengaard, Vibe-Pedersen, Sahl, Sottrup-Jensen (bib25) 1983; 80 ffrench-Constant, Hynes (bib6) 1989; 106 Muro, Iaconcig, Baralle (bib21) 1998; 437 Price, Hynes (bib28) 1985; 5 ffrench-Constant (bib5) 1995; 221 Fukamauchi, Mataga, Wang, Sato, Youshiki, Kusakabe (bib9) 1996; 221 George, Georges-Labouesse, Patel-King, Rayburn, Hynes (bib10) 1993; 119 Chauhan, Iaconcig, Baralle, Muro (bib3) 2004; 324 Pytela (10.1016/j.bbr.2005.02.020_bib29) 1985; 40 Border (10.1016/j.bbr.2005.02.020_bib1) 1992; 360 Petersen (10.1016/j.bbr.2005.02.020_bib25) 1983; 80 Muro (10.1016/j.bbr.2005.02.020_bib20) 2003; 162 Rovasio (10.1016/j.bbr.2005.02.020_bib30) 1983; 96 Mackie (10.1016/j.bbr.2005.02.020_bib19) 1999; 112 Porsolt (10.1016/j.bbr.2005.02.020_bib27) 1977; 266 Komblihtt (10.1016/j.bbr.2005.02.020_bib17) 1984; 3 Price (10.1016/j.bbr.2005.02.020_bib28) 1985; 5 Pagani (10.1016/j.bbr.2005.02.020_bib22) 1993; 1173 ffrench-Constant (10.1016/j.bbr.2005.02.020_bib5) 1995; 221 Fukamauchi (10.1016/j.bbr.2005.02.020_bib8) 1998; 32 Schwarzbauer (10.1016/j.bbr.2005.02.020_bib33) 1987; 6 Muro (10.1016/j.bbr.2005.02.020_bib21) 1998; 437 Freitag (10.1016/j.bbr.2005.02.020_bib7) 2003; 145 Guan (10.1016/j.bbr.2005.02.020_bib11) 1990; 60 Pires Neto (10.1016/j.bbr.2005.02.020_bib26) 1999; 32 Pagani (10.1016/j.bbr.2005.02.020_bib23) 1991; 113 Thiery (10.1016/j.bbr.2005.02.020_bib35) 1989 George (10.1016/j.bbr.2005.02.020_bib10) 1993; 119 Komblihtt (10.1016/j.bbr.2005.02.020_bib15) 1996; 10 Peters (10.1016/j.bbr.2005.02.020_bib24) 1996; 4 Jones (10.1016/j.bbr.2005.02.020_bib14) 2000; 218 ffrench-Constant (10.1016/j.bbr.2005.02.020_bib6) 1989; 106 Sakai (10.1016/j.bbr.2005.02.020_bib32) 2001; 7 Fukamauchi (10.1016/j.bbr.2005.02.020_bib9) 1996; 221 Chauhan (10.1016/j.bbr.2005.02.020_bib3) 2004; 324 Komblihtt (10.1016/j.bbr.2005.02.020_bib16) 1983; 80 Saga (10.1016/j.bbr.2005.02.020_bib31) 1992; 6 Chomczynski (10.1016/j.bbr.2005.02.020_bib4) 1987; 162 Buratti (10.1016/j.bbr.2005.02.020_bib2) 2004; 24 Hynes (10.1016/j.bbr.2005.02.020_bib12) 1986; 254 10.1016/j.bbr.2005.02.020_bib13 Venter (10.1016/j.bbr.2005.02.020_bib36) 2001; 291 Zardi (10.1016/j.bbr.2005.02.020_bib37) 1987; 6 Liao (10.1016/j.bbr.2005.02.020_bib18) 2002; 11 Schwarzbauer (10.1016/j.bbr.2005.02.020_bib34) 1983; 35 |
References_xml | – volume: 106 start-page: 375 year: 1989 end-page: 388 ident: bib6 article-title: Alternative splicing of fibronectin is temporally and spatially regulated in the chicken embryo publication-title: Development contributor: fullname: Hynes – volume: 162 start-page: 156 year: 1987 end-page: 159 ident: bib4 article-title: Single-step method of RNA isolation by acid guanidinium thiocyanate- phenol-chloroform extraction publication-title: Anal Biochem contributor: fullname: Sacchi – volume: 162 start-page: 149 year: 2003 end-page: 160 ident: bib20 article-title: Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan publication-title: J Cell Biol contributor: fullname: Stanta – volume: 291 start-page: 1304 year: 2001 end-page: 1351 ident: bib36 article-title: The sequence of the human genome publication-title: Science contributor: fullname: Venter – volume: 266 start-page: 730 year: 1977 end-page: 732 ident: bib27 article-title: Depression: a new animal model sensitive to antidepressant treatments publication-title: Nature contributor: fullname: Jalfre – volume: 221 start-page: 151 year: 1996 end-page: 156 ident: bib9 article-title: Abnormal behavior and neurotransmissions of tenascin gene knockout mouse publication-title: Biochem Biophys Res Commun contributor: fullname: Kusakabe – volume: 32 start-page: 633 year: 1999 end-page: 638 ident: bib26 article-title: Extracellular matrix molecules play diverse roles in the growth and guidance of central nervous system axons publication-title: Braz J Med Biol Res contributor: fullname: Lent – volume: 7 start-page: 324 year: 2001 end-page: 330 ident: bib32 article-title: Plasma fibronectin supports neuronal survival and reduces brain injury following transient focal cerebral ischemia but is not essential for skin-wound healing and hemostasis publication-title: Nat Med contributor: fullname: Sakai – volume: 4 start-page: 127 year: 1996 end-page: 148 ident: bib24 article-title: Fibronectin isoform distribution in the mouse. II. Differential distribution of the alternatively spliced EIIDB, EIIIA, and V segments in the adult mouse publication-title: Cell Adhes Commun contributor: fullname: Hynes – volume: 221 start-page: 261 year: 1995 end-page: 271 ident: bib5 article-title: Alternative splicing of fibronectin—many different proteins but few different functions publication-title: Exp Cell Res contributor: fullname: ffrench-Constant – volume: 96 start-page: 462 year: 1983 end-page: 473 ident: bib30 article-title: Neural crest cell migration: requirements for exogenous fibronectin and high cell density publication-title: J Cell Biol contributor: fullname: Thiery – volume: 80 start-page: 3218 year: 1983 end-page: 3222 ident: bib16 article-title: Isolation and characterization of cDNA clones for human and bovine fibronectins publication-title: Proc Natl Acad Sci USA contributor: fullname: Baralle – volume: 3 start-page: 221 year: 1984 end-page: 226 ident: bib17 article-title: Human fibronectin: molecular cloning evidence for two mRNA species differing by an internal segment coding for a structural domain publication-title: EMBO J contributor: fullname: Baralle – volume: 35 start-page: 421 year: 1983 end-page: 431 ident: bib34 article-title: Three different fibronectin mRNAs arise by alternative splicing within the coding region publication-title: Cell contributor: fullname: Hynes – volume: 24 start-page: 1387 year: 2004 end-page: 1400 ident: bib2 article-title: RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon publication-title: Mol Cell Biol contributor: fullname: Baralle – volume: 113 start-page: 1223 year: 1991 end-page: 1229 ident: bib23 article-title: Tissue-specific splicing pattern of fibronectin messenger RNA precursor during development and aging in rat publication-title: J Cell Biol contributor: fullname: Baralle – volume: 119 start-page: 1079 year: 1993 end-page: 1091 ident: bib10 article-title: Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin publication-title: Development contributor: fullname: Hynes – volume: 145 start-page: 189 year: 2003 end-page: 207 ident: bib7 article-title: Behavioral alterations in mice deficient for the extracellular matrix glycoprotein tenascin-R publication-title: Behav Brain Res contributor: fullname: Morellini – volume: 6 start-page: 2337 year: 1987 end-page: 2342 ident: bib37 article-title: Transformed human cells produce a new fibronectin isoform by preferential alternative splicing of a previously unobserved exon publication-title: EMBO J contributor: fullname: Sebastio – volume: 6 start-page: 2573 year: 1987 end-page: 2580 ident: bib33 article-title: Multiple sites of alternative splicing of the rat fibronectin gene transcript publication-title: EMBO J contributor: fullname: Hynes – volume: 112 start-page: 3847 year: 1999 end-page: 3853 ident: bib19 article-title: The tenascin-C knockout revisited publication-title: J Cell Sci contributor: fullname: Tucker – volume: 324 start-page: 55 year: 2004 end-page: 63 ident: bib3 article-title: Alternative splicing of fibronectin: a mouse model demonstrates the identity of in vitro and in vivo systems and the processing autonomy of regulated exons in adult mice publication-title: Gene contributor: fullname: Muro – volume: 254 start-page: 42 year: 1986 end-page: 51 ident: bib12 article-title: Fibronectins publication-title: Sci Am contributor: fullname: Hynes – volume: 360 start-page: 361 year: 1992 end-page: 364 ident: bib1 article-title: Natural inhibitor of transforming growth factor-beta protects against scarring in experimental kidney disease publication-title: Nature contributor: fullname: Pierschbacher – volume: 10 start-page: 248 year: 1996 end-page: 257 ident: bib15 article-title: The fibronectin gene as a model for splicing and transcription studies publication-title: Faseb J contributor: fullname: Werbajh – volume: 6 start-page: 1821 year: 1992 end-page: 1831 ident: bib31 article-title: Mice develop normally without tenascin publication-title: Genes Dev contributor: fullname: Aizawa – volume: 40 start-page: 191 year: 1985 end-page: 198 ident: bib29 article-title: Identification and isolation of a 140 publication-title: Cell contributor: fullname: Ruoslahti – volume: 218 start-page: 235 year: 2000 end-page: 259 ident: bib14 article-title: The tenascin family of ECM glycoproteins: structure, function, and regulation during embryonic development and tissue remodeling publication-title: Dev Dyn contributor: fullname: Jones – volume: 1173 start-page: 172 year: 1993 end-page: 178 ident: bib22 article-title: Expression and alternative splicing of fibronectin mRNA in human diploid endothelial cells during aging in vitro publication-title: Biochim Biophys Acta contributor: fullname: Vergani – volume: 437 start-page: 137 year: 1998 end-page: 141 ident: bib21 article-title: Regulation of the fibronectin EDA exon alternative splicing. Cooperative role of the exonic enhancer element and the 5′ splicing site publication-title: FEBS Lett contributor: fullname: Baralle – year: 1989 ident: bib35 article-title: Roles of fibronectin in embryogenesis contributor: fullname: Imhof – volume: 5 start-page: 2205 year: 1985 end-page: 2211 ident: bib28 article-title: Astrocytes in culture synthesize and secrete a variant form of fibronectin publication-title: J Neurosci contributor: fullname: Hynes – volume: 32 start-page: 461 year: 1998 end-page: 464 ident: bib8 article-title: The effects of central administration of neuropeptide Y on behavior of tenascin-gene knockout mice publication-title: Neuropeptides contributor: fullname: Kusakabe – volume: 11 start-page: 11 year: 2002 ident: bib18 article-title: The EIIIA segment of fibronectin is a ligand for integrins alpha 9beta 1 and alpha 4beta 1 providing a novel mechanism for regulating cell adhesion by alternative splicing publication-title: J Biol Chem contributor: fullname: Van De Water – volume: 60 start-page: 53 year: 1990 end-page: 61 ident: bib11 article-title: Lymphoid cells recognize an alternatively spliced segment of fibronectin via the integrin receptor alpha 4 beta 1 publication-title: Cell contributor: fullname: Hynes – volume: 80 start-page: 137 year: 1983 end-page: 141 ident: bib25 article-title: Partial primary structure of bovine plasma fibronectin: three types of internal homology publication-title: Proc Natl Acad Sci USA contributor: fullname: Sottrup-Jensen – volume: 254 start-page: 42 year: 1986 ident: 10.1016/j.bbr.2005.02.020_bib12 article-title: Fibronectins publication-title: Sci Am doi: 10.1038/scientificamerican0686-42 contributor: fullname: Hynes – volume: 324 start-page: 55 year: 2004 ident: 10.1016/j.bbr.2005.02.020_bib3 article-title: Alternative splicing of fibronectin: a mouse model demonstrates the identity of in vitro and in vivo systems and the processing autonomy of regulated exons in adult mice publication-title: Gene doi: 10.1016/j.gene.2003.09.026 contributor: fullname: Chauhan – volume: 145 start-page: 189 year: 2003 ident: 10.1016/j.bbr.2005.02.020_bib7 article-title: Behavioral alterations in mice deficient for the extracellular matrix glycoprotein tenascin-R publication-title: Behav Brain Res doi: 10.1016/S0166-4328(03)00109-8 contributor: fullname: Freitag – volume: 360 start-page: 361 year: 1992 ident: 10.1016/j.bbr.2005.02.020_bib1 article-title: Natural inhibitor of transforming growth factor-beta protects against scarring in experimental kidney disease publication-title: Nature doi: 10.1038/360361a0 contributor: fullname: Border – volume: 106 start-page: 375 year: 1989 ident: 10.1016/j.bbr.2005.02.020_bib6 article-title: Alternative splicing of fibronectin is temporally and spatially regulated in the chicken embryo publication-title: Development doi: 10.1242/dev.106.2.375 contributor: fullname: ffrench-Constant – volume: 80 start-page: 3218 year: 1983 ident: 10.1016/j.bbr.2005.02.020_bib16 article-title: Isolation and characterization of cDNA clones for human and bovine fibronectins publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.80.11.3218 contributor: fullname: Komblihtt – volume: 162 start-page: 156 year: 1987 ident: 10.1016/j.bbr.2005.02.020_bib4 article-title: Single-step method of RNA isolation by acid guanidinium thiocyanate- phenol-chloroform extraction publication-title: Anal Biochem doi: 10.1016/0003-2697(87)90021-2 contributor: fullname: Chomczynski – volume: 3 start-page: 221 year: 1984 ident: 10.1016/j.bbr.2005.02.020_bib17 article-title: Human fibronectin: molecular cloning evidence for two mRNA species differing by an internal segment coding for a structural domain publication-title: EMBO J doi: 10.1002/j.1460-2075.1984.tb01787.x contributor: fullname: Komblihtt – volume: 113 start-page: 1223 year: 1991 ident: 10.1016/j.bbr.2005.02.020_bib23 article-title: Tissue-specific splicing pattern of fibronectin messenger RNA precursor during development and aging in rat publication-title: J Cell Biol doi: 10.1083/jcb.113.5.1223 contributor: fullname: Pagani – volume: 24 start-page: 1387 year: 2004 ident: 10.1016/j.bbr.2005.02.020_bib2 article-title: RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon publication-title: Mol Cell Biol doi: 10.1128/MCB.24.3.1387-1400.2004 contributor: fullname: Buratti – volume: 6 start-page: 1821 year: 1992 ident: 10.1016/j.bbr.2005.02.020_bib31 article-title: Mice develop normally without tenascin publication-title: Genes Dev doi: 10.1101/gad.6.10.1821 contributor: fullname: Saga – volume: 80 start-page: 137 year: 1983 ident: 10.1016/j.bbr.2005.02.020_bib25 article-title: Partial primary structure of bovine plasma fibronectin: three types of internal homology publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.80.1.137 contributor: fullname: Petersen – volume: 40 start-page: 191 year: 1985 ident: 10.1016/j.bbr.2005.02.020_bib29 article-title: Identification and isolation of a 140kd cell surface glycoprotein with properties expected of a fibronectin receptor publication-title: Cell doi: 10.1016/0092-8674(85)90322-8 contributor: fullname: Pytela – volume: 291 start-page: 1304 year: 2001 ident: 10.1016/j.bbr.2005.02.020_bib36 article-title: The sequence of the human genome publication-title: Science doi: 10.1126/science.1058040 contributor: fullname: Venter – volume: 5 start-page: 2205 year: 1985 ident: 10.1016/j.bbr.2005.02.020_bib28 article-title: Astrocytes in culture synthesize and secrete a variant form of fibronectin publication-title: J Neurosci doi: 10.1523/JNEUROSCI.05-08-02205.1985 contributor: fullname: Price – volume: 437 start-page: 137 year: 1998 ident: 10.1016/j.bbr.2005.02.020_bib21 article-title: Regulation of the fibronectin EDA exon alternative splicing. Cooperative role of the exonic enhancer element and the 5′ splicing site publication-title: FEBS Lett doi: 10.1016/S0014-5793(98)01201-0 contributor: fullname: Muro – volume: 162 start-page: 149 year: 2003 ident: 10.1016/j.bbr.2005.02.020_bib20 article-title: Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan publication-title: J Cell Biol doi: 10.1083/jcb.200212079 contributor: fullname: Muro – volume: 6 start-page: 2337 year: 1987 ident: 10.1016/j.bbr.2005.02.020_bib37 article-title: Transformed human cells produce a new fibronectin isoform by preferential alternative splicing of a previously unobserved exon publication-title: EMBO J doi: 10.1002/j.1460-2075.1987.tb02509.x contributor: fullname: Zardi – volume: 6 start-page: 2573 year: 1987 ident: 10.1016/j.bbr.2005.02.020_bib33 article-title: Multiple sites of alternative splicing of the rat fibronectin gene transcript publication-title: EMBO J doi: 10.1002/j.1460-2075.1987.tb02547.x contributor: fullname: Schwarzbauer – volume: 221 start-page: 151 year: 1996 ident: 10.1016/j.bbr.2005.02.020_bib9 article-title: Abnormal behavior and neurotransmissions of tenascin gene knockout mouse publication-title: Biochem Biophys Res Commun doi: 10.1006/bbrc.1996.0561 contributor: fullname: Fukamauchi – volume: 10 start-page: 248 year: 1996 ident: 10.1016/j.bbr.2005.02.020_bib15 article-title: The fibronectin gene as a model for splicing and transcription studies publication-title: Faseb J doi: 10.1096/fasebj.10.2.8641558 contributor: fullname: Komblihtt – volume: 7 start-page: 324 year: 2001 ident: 10.1016/j.bbr.2005.02.020_bib32 article-title: Plasma fibronectin supports neuronal survival and reduces brain injury following transient focal cerebral ischemia but is not essential for skin-wound healing and hemostasis publication-title: Nat Med doi: 10.1038/85471 contributor: fullname: Sakai – volume: 1173 start-page: 172 year: 1993 ident: 10.1016/j.bbr.2005.02.020_bib22 article-title: Expression and alternative splicing of fibronectin mRNA in human diploid endothelial cells during aging in vitro publication-title: Biochim Biophys Acta doi: 10.1016/0167-4781(93)90178-G contributor: fullname: Pagani – volume: 32 start-page: 633 year: 1999 ident: 10.1016/j.bbr.2005.02.020_bib26 article-title: Extracellular matrix molecules play diverse roles in the growth and guidance of central nervous system axons publication-title: Braz J Med Biol Res doi: 10.1590/S0100-879X1999000500017 contributor: fullname: Pires Neto – volume: 119 start-page: 1079 year: 1993 ident: 10.1016/j.bbr.2005.02.020_bib10 article-title: Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin publication-title: Development doi: 10.1242/dev.119.4.1079 contributor: fullname: George – volume: 35 start-page: 421 year: 1983 ident: 10.1016/j.bbr.2005.02.020_bib34 article-title: Three different fibronectin mRNAs arise by alternative splicing within the coding region publication-title: Cell doi: 10.1016/0092-8674(83)90175-7 contributor: fullname: Schwarzbauer – volume: 32 start-page: 461 year: 1998 ident: 10.1016/j.bbr.2005.02.020_bib8 article-title: The effects of central administration of neuropeptide Y on behavior of tenascin-gene knockout mice publication-title: Neuropeptides doi: 10.1016/S0143-4179(98)90072-5 contributor: fullname: Fukamauchi – volume: 60 start-page: 53 year: 1990 ident: 10.1016/j.bbr.2005.02.020_bib11 article-title: Lymphoid cells recognize an alternatively spliced segment of fibronectin via the integrin receptor alpha 4 beta 1 publication-title: Cell doi: 10.1016/0092-8674(90)90715-Q contributor: fullname: Guan – year: 1989 ident: 10.1016/j.bbr.2005.02.020_bib35 contributor: fullname: Thiery – volume: 11 start-page: 11 year: 2002 ident: 10.1016/j.bbr.2005.02.020_bib18 article-title: The EIIIA segment of fibronectin is a ligand for integrins alpha 9beta 1 and alpha 4beta 1 providing a novel mechanism for regulating cell adhesion by alternative splicing publication-title: J Biol Chem contributor: fullname: Liao – volume: 4 start-page: 127 year: 1996 ident: 10.1016/j.bbr.2005.02.020_bib24 article-title: Fibronectin isoform distribution in the mouse. II. Differential distribution of the alternatively spliced EIIDB, EIIIA, and V segments in the adult mouse publication-title: Cell Adhes Commun doi: 10.3109/15419069609010767 contributor: fullname: Peters – volume: 218 start-page: 235 year: 2000 ident: 10.1016/j.bbr.2005.02.020_bib14 article-title: The tenascin family of ECM glycoproteins: structure, function, and regulation during embryonic development and tissue remodeling publication-title: Dev Dyn doi: 10.1002/(SICI)1097-0177(200006)218:2<235::AID-DVDY2>3.0.CO;2-G contributor: fullname: Jones – volume: 112 start-page: 3847 issue: Pt 22 year: 1999 ident: 10.1016/j.bbr.2005.02.020_bib19 article-title: The tenascin-C knockout revisited publication-title: J Cell Sci doi: 10.1242/jcs.112.22.3847 contributor: fullname: Mackie – volume: 221 start-page: 261 year: 1995 ident: 10.1016/j.bbr.2005.02.020_bib5 article-title: Alternative splicing of fibronectin—many different proteins but few different functions publication-title: Exp Cell Res doi: 10.1006/excr.1995.1374 contributor: fullname: ffrench-Constant – volume: 266 start-page: 730 year: 1977 ident: 10.1016/j.bbr.2005.02.020_bib27 article-title: Depression: a new animal model sensitive to antidepressant treatments publication-title: Nature doi: 10.1038/266730a0 contributor: fullname: Porsolt – volume: 96 start-page: 462 year: 1983 ident: 10.1016/j.bbr.2005.02.020_bib30 article-title: Neural crest cell migration: requirements for exogenous fibronectin and high cell density publication-title: J Cell Biol doi: 10.1083/jcb.96.2.462 contributor: fullname: Rovasio – ident: 10.1016/j.bbr.2005.02.020_bib13 doi: 10.1007/978-1-4612-3264-3 |
SSID | ssj0004051 |
Score | 1.9363648 |
Snippet | The extracellular matrix (ECM) plays an important role in the central nervous system (CNS) by modulating the migration of cells, axons and dendrites of... |
SourceID | proquest crossref pubmed pascalfrancis elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 31 |
SubjectTerms | Alternative splicing Alternative Splicing - physiology Analysis of Variance Animals Biological and medical sciences Blotting, Northern - methods Circadian Rhythm - physiology Exons Exploratory Behavior - physiology Extracellular matrix Fibronectin isoforms Fibronectins - chemistry Fibronectins - deficiency Fundamental and applied biological sciences. Psychology Mice Mice, Inbred C57BL Mice, Transgenic Motor Activity - physiology Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration Motor coordination and balance Protein Structure, Tertiary - genetics Psychomotor Disorders - genetics Psychomotor Performance - physiology Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - metabolism Rotarod Performance Test - methods Rotarod test Swimming Vertebrates: nervous system and sense organs |
Title | Impaired motor coordination in mice lacking the EDA exon of the fibronectin gene |
URI | https://dx.doi.org/10.1016/j.bbr.2005.02.020 https://www.ncbi.nlm.nih.gov/pubmed/15904707 https://search.proquest.com/docview/17593364 https://search.proquest.com/docview/19542682 https://search.proquest.com/docview/67845092 |
Volume | 161 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1La9wwEB6SzaVQStv0sX1sdSg9FNyVbFmWj0uasGlpKLSB3IxkSbCB2stmA-2lv72f_GDJYXMoCINtyWONpJlvRo8hep9bY4LUNilrAQOlViaxyJo4p7QpbCmtjTO63y7U8lJ-ucqvDuhk3AsTl1UOsr-X6Z20Hp7MB27O16vV_AfACtRpqnk801_p8pCOoI6knNDR4vzr8mK3PZLnfVhCpZJYYJzc7JZ5WbsZPCspEt-nnh6uzQ2YFvpoF_vhaKeWzh7TowFPskX_y0_owDdP6XjRwJb-9Yd9YN0Kz851fkzfzzH2UV3H0D7thtUtLM9V7w5kq4bFyPQsOvSgzhiAITv9vGD-N162obsPsK3bJorIhqHj-Wd0eXb682SZDBEVklrKbJuEaH0FQFRROJXDIA0FD8qkdfBZ7VxRp7aQXnhpA66wPZQwSjuXBqetgXB8TpMGdF4S006YEKw3XgYpNDcmzmGKTHqTC3xnSh9HRlbr_uCMalxRdl2B6zEAZl7xFIlPSY6sru60fgXBfl-x2Z1m2RGCCpY6lVN6N7ZThWET50JM49vbmwqoqcwydV-OMgd60en-HJEI8BZyvOi7wI5-XnJZ8OLV_9XrNT3oToiNzp7sDU22m1v_Fthna2d0-OmvmA09_B-FOwPu |
link.rule.ids | 315,786,790,4521,24144,27957,27958,45620,45714 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1La9wwEB7S9JBCKW2StttHokPIIeCuH7IsH5c0YdMmIZAEchOSJcEGai-bDbSX_vZ-8oMlh82hIAy2x5Y1kma-GY01RAe50dpzaaKySmCgVEJHBqSRtULqwpTcmLCie3Epprf8x11-t0HHw78wIayyl_2dTG-ldX9l3HNzPJ_NxtcAK1CnqYzDnv5Cli_oZYDzIX_Dt7-rOA8gki4poRBRIB-WNtsgL2MWvV8lRYnXKafXc_0Alvku18V6MNoqpdO39KZHk2zSffA72nD1Nu1MaljSv_6wQ9bGd7aO8x26OsPMR2MtQ-80C1Y1sDtnnTOQzWoW8tKz4M6DMmOAhezk-4S537jZ-Pbcw7Ju6iAga4Zh53bp9vTk5nga9fkUoorzbBn5YHt5ANSksCKHOeqL2AudVt5llbVFlZqCu8Rx43GE5SESLaS1qbfSaIjG97RZo56PxKRNtPfGacc9T2SsdVjBTDLudJ7gPSM6Ghip5t22GWqIJ7tX4HpIf5mrOEWJR8QHVqsnfa8g1p97bO9Jt6wqggLmMuUj2h_6SWHShJUQXbvm8UEBM5VZJp6jKHNgF5mupwiVAG2B4kM3BFb152XMi7j49H_t2qet6c3FuTo_u_z5mV61e8UGt0_2hTaXi0f3FShoafbaUf4PwZ8EvA |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Impaired+motor+coordination+in+mice+lacking+the+EDA+exon+of+the+fibronectin+gene&rft.jtitle=Behavioural+brain+research&rft.au=Chauhan%2C+Anil+Kumar&rft.au=Moretti%2C+Federico+Andrea&rft.au=Iaconcig%2C+Alessandra&rft.au=Baralle%2C+Francisco+Ernesto&rft.date=2005-06-03&rft.issn=0166-4328&rft.eissn=1872-7549&rft.volume=161&rft.issue=1&rft.spage=31&rft_id=info:doi/10.1016%2Fj.bbr.2005.02.020&rft_id=info%3Apmid%2F15904707&rft.externalDocID=15904707 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0166-4328&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0166-4328&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0166-4328&client=summon |