CRISPR-based targeting of DNA methylation in Arabidopsis thaliana by a bacterial CG-specific DNA methyltransferase

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 118; no. 23; pp. 1 - 8
• Main Authors: Ghoshal, Basudev, Picard, Colette L., Vong, Brandon, Feng, Suhua, Jacobsen, Steven E.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 08.06.2021
• Subjects: Arabidopsis - enzymology; Arabidopsis - genetics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological Sciences; CRISPR; CRISPR-Cas Systems; Cytosine; Deoxyribonucleic acid; DNA; DNA Methylation; DNA methyltransferase; DNA, Plant - genetics; DNA, Plant - metabolism; DNA-Cytosine Methylases - genetics; DNA-Cytosine Methylases - metabolism; Epigenetics; Gene expression; Gene silencing; Meiosis; Nucleotide sequence; Phenotypes; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Tenericutes - enzymology; Tenericutes - genetics; DNA methylation; SunTag; Arabidopsis; CRISPR-Cas9

CRISPR-based targeted modification of epigenetic marks such as DNA cytosine methylation is an important strategy to regulate the expression of genes and their associated phenotypes. Although plants have DNA methylation in all sequence contexts (CG, CHG, CHH, where H = A, T, C), methylation in the sy...