Development of an impedimetric immunosensor based on electropolymerized polytyramine films for the direct detection of Salmonella typhimurium in pure cultures of type strains and inoculated real samples

• Published in: Analytica chimica acta Vol. 624; no. 2; pp. 301 - 307
• Main Authors: Pournaras, Antonios V., Koraki, Theodora, Prodromidis, Mamas I.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 29.08.2008; Elsevier
• Subjects: Animals; Biological and medical sciences; Biosensors; Biotechnology; Cattle; Culture Media; Electric Impedance; Electrochemistry; Electropolymerized polytyramine films; Faradic electrochemical impedance spectroscopy; Fundamental and applied biological sciences. Psychology; Immunoassay - methods; Immunosensors; Methods. Procedures. Technologies; Milk - microbiology; On-site detection of pathogens; Polymers - chemical synthesis; Polymers - chemistry; Polymers - metabolism; Reproducibility of Results; Salmonella typhimurium; Salmonella typhimurium - classification; Salmonella typhimurium - isolation & purification; Salmonella typhimurium - metabolism; Sensitivity and Specificity; Tyramine - chemistry; Various methods and equipments; Electropolymerized polytyramine films; Immunosensors; Faradic electrochemical impedance spectroscopy; Salmonella typhimurium; On-site detection of pathogens; Cell culture; Gold; Stability; Glutaral; Electrochemical polymerization; Immunosensor; Sensitivity; Redox couple; Biosensor; Detection limit; Concentration effect; Hexacyanoferrates III; Bacteria; Capacitance; Hexacyanoferrates II; Aqueous solution; Enterobacteriaceae; Milk; Electrochemical impedance spectroscopy

The development of a faradic impedimetric immunosensor based on electropolymerized polytyramine (Ptyr) films for the detection of S. typhimurium in milk is described for the first time. Polyclonal anti- Salmonella was cross-linked, in the presence of glutaraldehyde vapors, on Ptyr-modified gold elec...