Integrated chromosomal and plasmid sequence analyses reveal diverse modes of carbapenemase gene spread among Klebsiella pneumoniae

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 117; no. 40; pp. 25043 - 25054
• Main Authors: David, Sophia, Cohen, Victoria, Reuter, Sandra, Sheppar, Anna E., Giani, Tommaso, Parkhill, Julian, Rossolini, Gian Maria, Feil, Edward J., Grundman, Hajo, Aanensen, David M.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 06.10.2020
• Subjects: Anti-Bacterial Agents - therapeutic use; Antibiotic resistance; Antibiotics; Bacterial Proteins - genetics; beta-Lactamases - genetics; Biological Sciences; Carbapenemase; Carbapenems; Carbapenems - therapeutic use; Cell Lineage - genetics; Chromosomes, Bacterial - genetics; Drug resistance; Drug Resistance, Multiple, Bacterial - drug effects; Drug Resistance, Multiple, Bacterial - genetics; Epidemics; Genes; Genome, Bacterial - genetics; Humans; Klebsiella; Klebsiella Infections - drug therapy; Klebsiella Infections - genetics; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - pathogenicity; Mathematical analysis; Pathogens; Plasmids; Plasmids - genetics; Recombination; Sequence Analysis, DNA - methods; Surveillance systems; genomics; carbapenemase genes; plasmids; carbapenem resistance; Klebsiella pneumoniae

Molecular and genomic surveillance systems for bacterial pathogens currently rely on tracking clonally evolving lineages. By contrast, plasmids are usually excluded or analyzed with low-resolution techniques, despite being the primary vectors of antibiotic resistance genes across many key pathogens....