An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice

• Published in: Frontiers in plant science Vol. 14; p. 1221790
• Main Authors: Tran, Hai Thanh, Schramm, Carly, Huynh, My-my, Shavrukov, Yuri, Stangoulis, James C. R., Jenkins, Colin L. D., Anderson, Peter A.
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 10.10.2023
• Subjects: homozygous plant; NOS terminator; OsSBE4 reference gene; Plant Science; quantitative real-time PCR; single T-DNA; zygosity identification

Early determination of transgenic plants that are homozygous for a single locus T-DNA insert is highly desirable in most fundamental and applied transgenic research. This study aimed to build on an accurate, rapid, and reliable quantitative real-time PCR (qPCR) method to fast-track the development o...