Nuclear Factor κB2 p52 Protein Has a Role in Antiviral Immunity through IκB Kinase ϵ-dependent Induction of Sp1 Protein and Interleukin 15

• Published in: The Journal of biological chemistry Vol. 288; no. 35; pp. 25066 - 25075
• Main Authors: Doyle, Sarah L., Shirey, Kari Ann, McGettrick, Anne F., Kenny, Elaine F., Carpenter, Susan, Caffrey, Brian E., Gargan, Siobhan, Quinn, Susan R., Caamaño, Jorge H., Moynagh, Paul, Vogel, Stefanie N., O'Neill, Luke A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.08.2013; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Cytokine Induction; Gene Expression Regulation - drug effects; Gene Expression Regulation - genetics; Gene Expression Regulation - immunology; Gene Regulation; HEK293 Cells; Humans; I-kappa B Kinase - genetics; I-kappa B Kinase - immunology; I-kappa B Kinase - metabolism; Innate Immunity; Interferon Inducers - pharmacology; Interleukin-15 - genetics; Interleukin-15 - metabolism; Mice; Mice, Knockout; Molecular Biology; NF-kappa B p52 Subunit - genetics; NF-kappa B p52 Subunit - immunology; NF-kappa B p52 Subunit - metabolism; NF-κB; Poly I-C - pharmacology; Respiratory Syncytial Virus Infections - genetics; Respiratory Syncytial Virus Infections - immunology; Respiratory Syncytial Virus Infections - metabolism; Respiratory Syncytial Virus Infections - pathology; Respiratory Syncytial Viruses - genetics; Respiratory Syncytial Viruses - immunology; Respiratory Syncytial Viruses - metabolism; Response Elements - genetics; Response Elements - immunology; Signal Transduction; Sp1; Sp1 Transcription Factor - biosynthesis; Sp1 Transcription Factor - genetics; Sp1 Transcription Factor - immunology; Toll-like Receptor (TLR); Toll-Like Receptor 3 - genetics; Toll-Like Receptor 3 - immunology; Toll-Like Receptor 3 - metabolism; Transcription; Transcription Factor RelA - genetics; Transcription Factor RelA - immunology; Transcription Factor RelA - metabolism; Viral Immunology; Gene Regulation; Cytokine Induction; NF-κB; Molecular Biology; Signal Transduction; Transcription; Sp1; Innate Immunity; Viral Immunology; Toll-like Receptor (TLR)

In this study we describe a previously unreported function for NFκB2, an NFκB family transcription factor, in antiviral immunity. NFκB2 is induced in response to poly(I:C), a mimic of viral dsRNA. Poly(I:C), acting via TLR3, induces p52-dependent transactivation of a reporter gene in a manner that r...