The aryl hydrocarbon receptor (AhR) activity and DNA-damaging effects of chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs)
An increasing number of studies have indicated that environmental contamination with chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs) has been underestimated. However, insufficient available toxicological information on Cl-PAHs makes evaluating their risks to health challenging. Two in vitro b...
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Published in | Chemosphere (Oxford) Vol. 211; pp. 640 - 647 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.11.2018
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Subjects | |
Online Access | Get full text |
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Summary: | An increasing number of studies have indicated that environmental contamination with chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs) has been underestimated. However, insufficient available toxicological information on Cl-PAHs makes evaluating their risks to health challenging. Two in vitro bioassays were used in the present study to characterize the aryl hydrocarbon receptor (AhR) activity and DNA-damaging effects of 22 low-molecular-weight PAHs and their Cl-PAHs by using the EROD assay in rat hepatoma (H4IIE) cells and the SOS/umu test (S. typhimurium TA1535/pSK1002). Compared with their parent PAHs, most of the Cl-PAHs enhanced AhR-mediated activity in the EROD assay. 1,3,6,8-Tetrachloro-pyrene (1,3,6,8-Tetracl-Py) induced the greatest potency of EROD activity (83.1%-TCDD-max) and its single point ReP was 6.64 × 10−6. Compared with their parent PAHs, several Cl-PAHs showed significant DNA-damaging effects in the SOS/umu test with the addition of S9, and the toxic equivalency of benzo[a]pyrene (TEQBaP) was calculated for them. 9-Chloroanthracene (9-Ant) and 5,6-Dichloroacenaphthene (5,6-Dicl-Ace) had relatively high TEQBaP (0.62 and 0.54, respectively). However, only 1,3,6,8-Tetracl-Py elicited strong DNA-damaging effects in the absence of S9. The degree of chlorination, the position of chlorine substitutions, and the structure of parent PAHs influenced the potency of low-molecular-weight PAHs with regard to their AhR activity and DNA-damaging effects. More concern should be raised for these environmentally relevant pollutants.
•Cl-PAHs had more potent AhR activity and DNA-damaging effects than their parent PAHs.•2,5-Dicl-Fl, 2,7-Dicl-Fl and 1,3,6,8-tetracl-Py induced strong EROD activity.•Degree of chlorination and the position of chlorine influence the AhR activity and DNA-damaging effects. |
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ISSN: | 0045-6535 1879-1298 |
DOI: | 10.1016/j.chemosphere.2018.07.087 |