Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells

• Published in: Food science and human wellness Vol. 13; no. 4; pp. 1929 - 1942
• Main Authors: Liu, Rui, Chu, Fangxin, Yan, Zheng, Chen, Hanqing
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.07.2024; Tsinghua University Press
• Subjects: alcohols; Alkali extraction; arabinose; chromatography; food science; galactose; humans; hydrogen peroxide; Opuntia dillenii Haw. fruits; Opuntia stricta var. dillenii; Oxidative stress; pectins; Polysaccharide; protective effect; rhamnose; Structural characterization; Oxidative stress; Opuntia dillenii Haw. fruits; Polysaccharide; Alkali extraction; Structural characterization

Three novel acidic polysaccharide fractions (OFPP-1, OFPP-2, OFPP-3) with different molecular weights (803.7, 555.1 and 414.5 kDa) were isolated from the peeled Opuntia dillenii Haw. fruits by alkali-extraction, graded alcohol precipitation and column chromatography. Structural analysis indicated th...