Quality assessment of DNA and hemoglobin by Fourier transform infrared spectroscopy in occupational exposure to extremely low-frequency magnetic field

• Published in: Environmental science and pollution research international Vol. 27; no. 36; pp. 45374 - 45380
• Main Authors: Zendehdel, Rezvan, Asadi, Sareh, Alizadeh, Somayeh, Ranjbarian, Mouhammad
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2020; Springer Nature B.V
• Subjects: Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; blood; Deoxyribonucleic acid; DNA; DNA structure; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Exposure; Fourier transform infrared spectroscopy; Fourier transforms; Functional groups; Hematopoietic system; Hemoglobin; human health; Infrared spectroscopy; Low frequency; Magnetic fields; Occupational exposure; Occupational health; people; Population studies; Quality assessment; Quality control; Research Article; socioeconomic status; Socioeconomics; Spectrum analysis; Toxicity; Waste Water Technology; Water Management; Water Pollution Control; Hemoglobin; Fourier transform infrared; Magnetic field; Extremely low frequency; DNA
• ISSN: 0944-1344; 1614-7499; 1614-7499
• DOI: 10.1007/s11356-020-09503-8

Previous studies have shown the effect of extremely low-frequency (ELF) magnetic fields on the hematopoietic system. However, molecular modification and biological toxicity are not known yet. The aim of this study was to investigate the effect of occupational exposure to ELF magnetic field on the hemoglobin and DNA alteration using Fourier transform infrared (FTIR) spectroscopy. Twenty nine individuals were selected among those working as the controller in a powerhouse in order to be studied as the population exposed to ELF magnetic field. Control group comprised of 29 administrative employees voluntarily participated who were matched with the exposed subjects in terms of sex, age, work experiences, smoking habit, and socioeconomic status. DNA and hemoglobin were extracted from blood samples and then were studied by FTIR spectroscopy. The results showed the level of magnetic field exposure was between 0.38 to 50 μT in the exposed subjects while the level of magnetic field exposure was between 0.19 and 20 μT for the unexposed people. Hemoglobin level was equal to 15.67 ± 1.42 g/dL for exposed subjects which is significantly lower than that of the unexposed people ( p  = 0.0001). There was a significant alteration in CH content and COO structure of the hemoglobin structure. Moreover, DNA showed significant changes by functional group of organic base. This change in the structure of DNA and hemoglobin can lead to the creation of risks in human health. In conclusion, FTIR method could reveal the quality of DNA and hemoglobin structure in subjects after exposure to ELF magnetic field.