A Diverse Set of Single-domain Antibodies (VHHs) against the Anthrax Toxin Lethal and Edema Factors Provides a Basis for Construction of a Bispecific Agent That Protects against Anthrax Infection

• Published in: The Journal of biological chemistry Vol. 291; no. 41; pp. 21596 - 21606
• Main Authors: Vrentas, Catherine E., Moayeri, Mahtab, Keefer, Andrea B., Greaney, Allison J., Tremblay, Jacqueline, O'Mard, Danielle, Leppla, Stephen H., Shoemaker, Charles B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.10.2016; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Anthrax - drug therapy; Anthrax - immunology; Anthrax - pathology; anthrax toxin; Antibodies, Bacterial - immunology; Antibodies, Bacterial - pharmacology; Antibodies, Bispecific - immunology; Antibodies, Bispecific - pharmacology; Antibodies, Neutralizing - immunology; Antibodies, Neutralizing - pharmacology; antibody; Antigens, Bacterial - immunology; Bacillus anthracis - immunology; Bacterial Toxins - antagonists & inhibitors; Bacterial Toxins - immunology; Camelids, New World; epitope mapping; Female; Mice; microbial pathogenesis; Molecular Bases of Disease; phage display; RAW 264.7 Cells; Single-Domain Antibodies - immunology; Single-Domain Antibodies - pharmacology; toxin; VHH; VNA; anthrax toxin; EF; antibody; toxin; phage display; VHH; VNA; LF; microbial pathogenesis; epitope mapping

Infection with Bacillus anthracis, the causative agent of anthrax, can lead to persistence of lethal secreted toxins in the bloodstream, even after antibiotic treatment. VHH single-domain antibodies have been demonstrated to neutralize diverse bacterial toxins both in vitro and in vivo, with protein...