Baicalin reduces the permeability of the blood–brain barrier during hypoxia in vitro by increasing the expression of tight junction proteins in brain microvascular endothelial cells

• Published in: Journal of ethnopharmacology Vol. 141; no. 2; pp. 714 - 720
• Main Authors: Zhu, Haiyan, Wang, Zhiyao, Xing, Yanwei, Gao, Yonghong, Ma, Tao, Lou, Lixia, Lou, Jinning, Gao, Ying, Wang, Shuoren, Wang, Yongyan
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.06.2012
• Subjects: Animals; Baicalin; Blood-Brain Barrier - drug effects; Blood-Brain Barrier - metabolism; Blood–brain barrier; Blotting, Western; brain; Capillary Permeability - drug effects; Cell Hypoxia; Cells, Cultured; Claudin-5; Claudins - genetics; Claudins - metabolism; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; edema; Electric Impedance; electrical resistance; endothelial cells; Endothelial Cells - drug effects; Endothelial Cells - metabolism; flavonoids; Flavonoids - isolation & purification; Flavonoids - pharmacology; Fluorescence; glucose; Glucose - deficiency; hypoxia; in vitro culture; Ischemia; Medicine, Chinese Traditional; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; Mice, Inbred BALB C; Microvessels - drug effects; Microvessels - metabolism; oxygen; permeability; Phosphoproteins - genetics; Phosphoproteins - metabolism; Plant Roots; Plants, Medicinal; protein kinase C; Protein Kinase C - metabolism; protein synthesis; proteins; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; roots; Scutellaria baicalensis; Scutellaria baicalensis - chemistry; tight junctions; Tight Junctions - drug effects; Tight Junctions - metabolism; Time Factors; traditional medicine; Transcription, Genetic - drug effects; Western blotting; Zonula occludens-1; Zonula Occludens-1 Protein; Claudin-5; Zonula occludens-1; Ischemia; Baicalin; Blood–brain barrier

Baicalin is one of the principal flavonoids isolated from the dried root of Scutellariae Baicalensis Georgi and has been widely used as a traditional herbal medicine to suppress brain edema and reduce cerebral ischemic damage. However, the effects of baicalin on the blood–brain barrier (BBB) are poo...