Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA‐induced gene knockouts

• Published in: The New phytologist Vol. 231; no. 4; pp. 1478 - 1495
• Main Authors: Xu, Wenjing, Cheng, Hao, Zhu, Siran, Cheng, Jiyao, Ji, Huanhuan, Zhang, Baocai, Cao, Shenquan, Wang, Chong, Tong, Guimin, Zhen, Cheng, Mu, Liqiang, Zhou, Yihua, Cheng, Yuxiang
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.08.2021; John Wiley and Sons Inc
• Subjects: Abnormalities; Biosynthesis; Cas9; Cell Wall - enzymology; Cell walls; Cellulose; Cellulose - metabolism; Cellulose synthase; cellulose synthase (CesA); CRISPR-Cas Systems; Fibers; gelatinous layer (G‐layer); gene knockout; Gene Knockout Techniques; Genes; Genetic abnormalities; Glucosyltransferases - metabolism; gRNA; Mutants; Phloem; phloem fibre; Polysaccharides; Populus - enzymology; Populus - genetics; Populus trichocarpa; RNA, Guide, CRISPR-Cas Systems; secondary cell wall (SCW); tension wood; Wood; Wood - metabolism; gene knockout; secondary cell wall (SCW); phloem fibre; cellulose synthase (CesA); Cas9; Populus trichocarpa; gelatinous layer (G-layer); gRNA; tension wood

Summary Plant cellulose is synthesized by a large plasma membrane‐localized cellulose synthase (CesA) complex. However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts. Here, the Cas9/gRNA‐induced knockouts of P...