Droplet digital PCR of tumor suppressor gene methylation in serial oral rinses of patients with head and neck squamous cell carcinoma
Background Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer‐related traits in body fluid. Methods Methylation of tumor suppressor genes (TSGs) (PAX5, EDNRB, and DCC) were measured in the oral rinses from 50 HNSCC and 58 control subjects using drople...
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| Published in | Head & neck Vol. 43; no. 6; pp. 1812 - 1822 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Hoboken, USA
John Wiley & Sons, Inc
01.06.2021
Wiley Subscription Services, Inc |
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| Abstract | Background
Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer‐related traits in body fluid.
Methods
Methylation of tumor suppressor genes (TSGs) (PAX5, EDNRB, and DCC) were measured in the oral rinses from 50 HNSCC and 58 control subjects using droplet digital PCR (ddPCR). Diagnostic accuracies in detecting HNSCC and the detection rate of recurrence in the post‐treatment monitoring were analyzed.
Results
ddPCR TSG methylation detection in oral rinses for diagnosis of HNSCC had an AUC of 0.892 for PAX5, 0.753 for EDNRB, and 0.729 for DCC. Significant drop of TSG methylation was observed after completion of surgery (p < 0.01). 76.9% of the relapse cases had a pre‐emptive rebound of methylation above presurgery levels in at least one of the tested markers before confirmed recurrence.
Conclusions
Utilizing ddPCR for TSG methylation detection in oral rinses shows potential for detection and monitoring of HNSCC. |
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| AbstractList | Background
Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer‐related traits in body fluid.
Methods
Methylation of tumor suppressor genes (TSGs) (PAX5, EDNRB, and DCC) were measured in the oral rinses from 50 HNSCC and 58 control subjects using droplet digital PCR (ddPCR). Diagnostic accuracies in detecting HNSCC and the detection rate of recurrence in the post‐treatment monitoring were analyzed.
Results
ddPCR TSG methylation detection in oral rinses for diagnosis of HNSCC had an AUC of 0.892 for PAX5, 0.753 for EDNRB, and 0.729 for DCC. Significant drop of TSG methylation was observed after completion of surgery (p < 0.01). 76.9% of the relapse cases had a pre‐emptive rebound of methylation above presurgery levels in at least one of the tested markers before confirmed recurrence.
Conclusions
Utilizing ddPCR for TSG methylation detection in oral rinses shows potential for detection and monitoring of HNSCC. BackgroundHead and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer‐related traits in body fluid.MethodsMethylation of tumor suppressor genes (TSGs) (PAX5, EDNRB, and DCC) were measured in the oral rinses from 50 HNSCC and 58 control subjects using droplet digital PCR (ddPCR). Diagnostic accuracies in detecting HNSCC and the detection rate of recurrence in the post‐treatment monitoring were analyzed.ResultsddPCR TSG methylation detection in oral rinses for diagnosis of HNSCC had an AUC of 0.892 for PAX5, 0.753 for EDNRB, and 0.729 for DCC. Significant drop of TSG methylation was observed after completion of surgery (p < 0.01). 76.9% of the relapse cases had a pre‐emptive rebound of methylation above presurgery levels in at least one of the tested markers before confirmed recurrence.ConclusionsUtilizing ddPCR for TSG methylation detection in oral rinses shows potential for detection and monitoring of HNSCC. Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer-related traits in body fluid.BACKGROUNDHead and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer-related traits in body fluid.Methylation of tumor suppressor genes (TSGs) (PAX5, EDNRB, and DCC) were measured in the oral rinses from 50 HNSCC and 58 control subjects using droplet digital PCR (ddPCR). Diagnostic accuracies in detecting HNSCC and the detection rate of recurrence in the post-treatment monitoring were analyzed.METHODSMethylation of tumor suppressor genes (TSGs) (PAX5, EDNRB, and DCC) were measured in the oral rinses from 50 HNSCC and 58 control subjects using droplet digital PCR (ddPCR). Diagnostic accuracies in detecting HNSCC and the detection rate of recurrence in the post-treatment monitoring were analyzed.ddPCR TSG methylation detection in oral rinses for diagnosis of HNSCC had an AUC of 0.892 for PAX5, 0.753 for EDNRB, and 0.729 for DCC. Significant drop of TSG methylation was observed after completion of surgery (p < 0.01). 76.9% of the relapse cases had a pre-emptive rebound of methylation above presurgery levels in at least one of the tested markers before confirmed recurrence.RESULTSddPCR TSG methylation detection in oral rinses for diagnosis of HNSCC had an AUC of 0.892 for PAX5, 0.753 for EDNRB, and 0.729 for DCC. Significant drop of TSG methylation was observed after completion of surgery (p < 0.01). 76.9% of the relapse cases had a pre-emptive rebound of methylation above presurgery levels in at least one of the tested markers before confirmed recurrence.Utilizing ddPCR for TSG methylation detection in oral rinses shows potential for detection and monitoring of HNSCC.CONCLUSIONSUtilizing ddPCR for TSG methylation detection in oral rinses shows potential for detection and monitoring of HNSCC. Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer-related traits in body fluid. Methylation of tumor suppressor genes (TSGs) (PAX5, EDNRB, and DCC) were measured in the oral rinses from 50 HNSCC and 58 control subjects using droplet digital PCR (ddPCR). Diagnostic accuracies in detecting HNSCC and the detection rate of recurrence in the post-treatment monitoring were analyzed. ddPCR TSG methylation detection in oral rinses for diagnosis of HNSCC had an AUC of 0.892 for PAX5, 0.753 for EDNRB, and 0.729 for DCC. Significant drop of TSG methylation was observed after completion of surgery (p < 0.01). 76.9% of the relapse cases had a pre-emptive rebound of methylation above presurgery levels in at least one of the tested markers before confirmed recurrence. Utilizing ddPCR for TSG methylation detection in oral rinses shows potential for detection and monitoring of HNSCC. |
| Author | Lam, Jacky W. K. Ng, Cherrie W. K. Fung, Sherwood Y. H. Cho, Ryan Wong, Eddy W. Y. Yeung, Zenon W. C. Chan, Jason Y. K. Chan, Kwan Chee Allen |
| AuthorAffiliation | 1 Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong Hong Kong SAR China 2 Department of Chemical Pathology Prince of Wales Hospital, The Chinese University of Hong Kong Hong Kong SAR China 4 Department of Otorhinolaryngology – Head and Neck Surgery Prince of Wales Hospital, The Chinese University of Hong Kong Hong Kong SAR China 3 State Key Laboratory of Translational Oncology, Sir Y.K. Pao Centre for Cancer Prince of Wales Hospital, The Chinese University of Hong Kong Hong Kong SAR China |
| AuthorAffiliation_xml | – name: 4 Department of Otorhinolaryngology – Head and Neck Surgery Prince of Wales Hospital, The Chinese University of Hong Kong Hong Kong SAR China – name: 2 Department of Chemical Pathology Prince of Wales Hospital, The Chinese University of Hong Kong Hong Kong SAR China – name: 1 Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong Hong Kong SAR China – name: 3 State Key Laboratory of Translational Oncology, Sir Y.K. Pao Centre for Cancer Prince of Wales Hospital, The Chinese University of Hong Kong Hong Kong SAR China |
| Author_xml | – sequence: 1 givenname: Sherwood Y. H. orcidid: 0000-0002-0956-9530 surname: Fung fullname: Fung, Sherwood Y. H. organization: Prince of Wales Hospital, The Chinese University of Hong Kong – sequence: 2 givenname: Kwan Chee Allen surname: Chan fullname: Chan, Kwan Chee Allen organization: Prince of Wales Hospital, The Chinese University of Hong Kong – sequence: 3 givenname: Eddy W. Y. surname: Wong fullname: Wong, Eddy W. Y. organization: Prince of Wales Hospital, The Chinese University of Hong Kong – sequence: 4 givenname: Cherrie W. K. surname: Ng fullname: Ng, Cherrie W. K. organization: Prince of Wales Hospital, The Chinese University of Hong Kong – sequence: 5 givenname: Ryan orcidid: 0000-0002-9162-282X surname: Cho fullname: Cho, Ryan organization: Prince of Wales Hospital, The Chinese University of Hong Kong – sequence: 6 givenname: Zenon W. C. surname: Yeung fullname: Yeung, Zenon W. C. organization: Prince of Wales Hospital, The Chinese University of Hong Kong – sequence: 7 givenname: Jacky W. K. surname: Lam fullname: Lam, Jacky W. K. organization: Prince of Wales Hospital, The Chinese University of Hong Kong – sequence: 8 givenname: Jason Y. K. orcidid: 0000-0002-9480-4637 surname: Chan fullname: Chan, Jason Y. K. email: jasonchan@ent.cuhk.edu.hk organization: Prince of Wales Hospital, The Chinese University of Hong Kong |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33594807$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1002_1878_0261_13708 crossref_primary_10_3390_cancers15072051 crossref_primary_10_3892_or_2024_8711 crossref_primary_10_1016_j_heliyon_2025_e42286 crossref_primary_10_1051_medsci_2022082 crossref_primary_10_1245_s10434_021_09904_0 crossref_primary_10_1016_j_oraloncology_2022_105733 crossref_primary_10_1002_cam4_70528 crossref_primary_10_1158_1078_0432_CCR_24_0590 crossref_primary_10_1021_acsbiomaterials_4c00606 |
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| Snippet | Background
Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer‐related traits in body fluid.
Methods... Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer-related traits in body fluid. Methylation of tumor... BackgroundHead and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer‐related traits in body fluid.MethodsMethylation... Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer-related traits in body fluid.BACKGROUNDHead and neck... |
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| SubjectTerms | DNA methylation droplet digital PCR Head & neck cancer head and neck cancer head and neck squamous cell carcinoma liquid biopsy methylation Original Pax5 protein Squamous cell carcinoma Surgery Tumor suppressor genes |
| Title | Droplet digital PCR of tumor suppressor gene methylation in serial oral rinses of patients with head and neck squamous cell carcinoma |
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