Simplified 2-D CE-MS mapping: Analysis of proteolytic digests
It has been demonstrated that CE‐MS is a very useful hyphenated technique for proteomic studies. However, the huge amount of data stored in a single CE‐MS run makes it necessary to account with procedures able to extract all the relevant information made available by CE‐MS. In this work, we present...
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Published in | Electrophoresis Vol. 28; no. 9; pp. 1335 - 1344 |
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Main Authors | , |
Format | Journal Article |
Language | English |
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Weinheim
WILEY-VCH Verlag
01.05.2007
WILEY‐VCH Verlag |
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Abstract | It has been demonstrated that CE‐MS is a very useful hyphenated technique for proteomic studies. However, the huge amount of data stored in a single CE‐MS run makes it necessary to account with procedures able to extract all the relevant information made available by CE‐MS. In this work, we present a new and easy approach capable of generating a simplified 2‐D map from CE‐MS raw data. This new approach provides the automatic detection and characterization of the most abundant ions from the CE‐MS data including their mass‐to‐charge (m/z) values, ion intensities and analysis times. It is demonstrated that visualization of CE‐MS data in this simplified 2‐D format allows: (i) an easy and simultaneous visual inspection of large datasets, (ii) an immediate perception of relevant differences in closely related samples, (iii) a rapid monitoring of data quality levels in different samples, and (iv) a fast discrimination between comigrating polypeptides and ESI‐MS fragmentation ions. The strategy proposed in this work does not rely on an excellent mass accuracy for peak detection and filtering, since MS values obtained from an IT analyzer are used. Moreover, the methodology developed works directly with the CE‐MS raw data, without interference by the user, giving simultaneously a simplified 2‐D map and a much easier and more complete data evaluation. Besides, this procedure can easily be implemented in any CE‐MS laboratory. The usefulness of this approach is validated by studying the very similar trypsin digests from bovine, rabbit and horse cytochrome c. It is demonstrated that this simplified 2‐D approach allows specific markers for each species to be obtained in a fast and simple way. |
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AbstractList | Abstract
It has been demonstrated that CE‐MS is a very useful hyphenated technique for proteomic studies. However, the huge amount of data stored in a single CE‐MS run makes it necessary to account with procedures able to extract all the relevant information made available by CE‐MS. In this work, we present a new and easy approach capable of generating a simplified 2‐D map from CE‐MS raw data. This new approach provides the automatic detection and characterization of the most abundant ions from the CE‐MS data including their mass‐to‐charge (
m/z
) values, ion intensities and analysis times. It is demonstrated that visualization of CE‐MS data in this simplified 2‐D format allows: (i) an easy and simultaneous visual inspection of large datasets, (ii) an immediate perception of relevant differences in closely related samples, (iii) a rapid monitoring of data quality levels in different samples, and (iv) a fast discrimination between comigrating polypeptides and ESI‐MS fragmentation ions. The strategy proposed in this work does not rely on an excellent mass accuracy for peak detection and filtering, since MS values obtained from an IT analyzer are used. Moreover, the methodology developed works directly with the CE‐MS raw data, without interference by the user, giving simultaneously a simplified 2‐D map and a much easier and more complete data evaluation. Besides, this procedure can easily be implemented in any CE‐MS laboratory. The usefulness of this approach is validated by studying the very similar trypsin digests from bovine, rabbit and horse cytochrome
c
. It is demonstrated that this simplified 2‐D approach allows specific markers for each species to be obtained in a fast and simple way. It has been demonstrated that CE-MS is a very useful hyphenated technique for proteomic studies. However, the huge amount of data stored in a single CE-MS run makes it necessary to account with procedures able to extract all the relevant information made available by CE-MS. In this work, we present a new and easy approach capable of generating a simplified 2-D map from CE-MS raw data. This new approach provides the automatic detection and characterization of the most abundant ions from the CE-MS data including their mass-to-charge (m/z) values, ion intensities and analysis times. It is demonstrated that visualization of CE-MS data in this simplified 2-D format allows: (i) an easy and simultaneous visual inspection of large datasets, (ii) an immediate perception of relevant differences in closely related samples, (iii) a rapid monitoring of data quality levels in different samples, and (iv) a fast discrimination between comigrating polypeptides and ESI-MS fragmentation ions. The strategy proposed in this work does not rely on an excellent mass accuracy for peak detection and filtering, since MS values obtained from an IT analyzer are used. Moreover, the methodology developed works directly with the CE-MS raw data, without interference by the user, giving simultaneously a simplified 2-D map and a much easier and more complete data evaluation. Besides, this procedure can easily be implemented in any CE-MS laboratory. The usefulness of this approach is validated by studying the very similar trypsin digests from bovine, rabbit and horse cytochrome c. It is demonstrated that this simplified 2-D approach allows specific markers for each species to be obtained in a fast and simple way. |
Author | Erny, Guillaume L. Cifuentes, Alejandro |
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Cites_doi | 10.1016/j.mce.2004.10.010 10.1016/S0021-9673(00)00585-9 10.1016/0021-9673(94)80083-9 10.1002/elps.200500527 10.1186/1471-2105-6-179 10.1002/elps.200500567 10.1016/j.jchromb.2004.07.045 10.1021/ac035375s 10.1016/S0021-9673(99)00299-X 10.1074/mcp.R200007-MCP200 10.1002/jssc.200500013 10.1002/1522-2683(200109)22:17<3629::AID-ELPS3629>3.0.CO;2-O 10.1016/j.meatsci.2004.12.004 10.2116/analsci.21.5 10.1016/j.cbpa.2005.12.017 10.1002/pmic.200401244 10.1016/j.jchromb.2005.12.051 10.1021/ac051067d 10.1016/0021-9673(96)00151-3 10.1002/elps.200390105 10.1016/S0021-9673(03)00713-1 10.1002/elps.200500474 10.1002/elps.200410130 |
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References | Dolník, V., Electrophoresis 2006, 27, 126-141. Survay, M. A., Goodall, D. M., Wren, S. A. C., Rowe, R. C., J. Chromatogr. A, 1996, 741, 99-113. Issaq, H. J., Electrophoresis 2001, 22, 3629-3638. Monton, M. R. N., Terabe, S., Anal. Sci. 2005, 21, 5-13. Palagi, P. M., Walther, D., Quadroni, M. et al., Proteomics 2005, 5, 2381-2384. Kašička, V., Electrophoresis 2006, 27, 142-175. Li, X. J., Pedrioli, P. G., Eng, J., et al. Anal. Chem. 2004, 76, 3856-3860. Anderson, N. L., Anderson, N. G., Moll. Cell. Proteomics. 2002, 1, 845-867. Dyson, N., Chromatographic Integration Methods, The Royal Society of Chemistry, Cambridge 1990. Xiao, Z., Prieto, D., Conrads, T. P., Timothy, D. et al., Mol. Cell. Endocrinol. 2005, 230, 95-106. Wittke, S., Fliser, D., Haubitz, M. et al., J Chromatogr. A 2003, 1013, 173-181. Simó, C., Gonzalez, R., Barbas, C., Cifuentes, A., Anal. Chem. 2005, 77, 7709-7716. Stutz, H., Electrophoresis 2005, 26, 1254-1290. Simó, C., Cifuentes, A., Electrophoresis 2003, 24, 834-842. Cifuentes, A., Electrophoresis 2006, 27, 283-303. Pietrogrande, M. C., Marchetti, N., Dondi, F., Righetti, P. G., J. Chromatogr. B 2006, 833, 51-62. Wilson, I. D., Plumb, R., Granger, J., Major, H. et al., J. Chromatogr. B 2005, 817, 67-76. Katajamaa, M., Oresic, M., BMC: Bioinformatics 2005, 6, art. no. 179. Dyson, N., J. Chromatogr. A 1999, 842, 321-340. Girish, P. S., Anjaneyulu, A. S. R., Viswas, K. N., Shivakumar, B. M. et al., Meat Sci. 2005, 70, 107-112. Vallejo-Cordoba, B., Gonzalez-Cordova, A. F., Mazorra-Manzano, M. A., Rodriguez-Ramirez, R., J. Sep. Sci. 2005, 28, 826-836. Issaq, H. J., Chan, K. C., Janini, G. M., Conrads, T. P. et al., J. Chromatogr. A 2005, 817, 35-47. Cifuentes, A., Poppe, H., J. Chromatogr. A 1994, 680, 321-340. Roesli, C., Elia, G., Neri, D., Curr. Opin. Chem. Biol. 2006, 10, 35-41. Ikuta, N., Yamada, Y., Yoshiyama, T., Hirokawa, T., J. Chromatogr. A, 2000, 894, 11-17. 2004; 76 1990 2000; 894 2006; 10 2006; 27 1994; 680 2003; 1013 2002; 1 2003; 24 2005; 21 2005; 6 2005 2005; 70 2005; 817 2001; 22 2005; 26 1996; 741 2006; 833 2005; 28 1999; 842 2005; 77 Dyson N. (e_1_2_1_17_2) 1990 e_1_2_1_22_2 e_1_2_1_23_2 e_1_2_1_20_2 e_1_2_1_21_2 e_1_2_1_26_2 e_1_2_1_24_2 e_1_2_1_25_2 Issaq H. J. (e_1_2_1_5_2) 2005; 817 e_1_2_1_6_2 e_1_2_1_7_2 e_1_2_1_4_2 e_1_2_1_2_2 e_1_2_1_11_2 e_1_2_1_3_2 e_1_2_1_12_2 e_1_2_1_10_2 e_1_2_1_15_2 e_1_2_1_16_2 e_1_2_1_13_2 e_1_2_1_14_2 e_1_2_1_19_2 e_1_2_1_8_2 e_1_2_1_9_2 e_1_2_1_18_2 |
References_xml | – volume: 833 start-page: 51 year: 2006 end-page: 62 publication-title: J. Chromatogr. B – volume: 27 start-page: 126 year: 2006 end-page: 141 publication-title: Electrophoresis – volume: 842 start-page: 321 year: 1999 end-page: 340 publication-title: J. Chromatogr. A – volume: 77 start-page: 7709 year: 2005 end-page: 7716 publication-title: Anal. Chem. – volume: 28 start-page: 826 year: 2005 end-page: 836 publication-title: J. Sep. Sci. – volume: 22 start-page: 3629 year: 2001 end-page: 3638 publication-title: Electrophoresis – year: 1990 – volume: 741 start-page: 99 year: 1996 end-page: 113 publication-title: J. Chromatogr. A – volume: 894 start-page: 11 year: 2000 end-page: 17 publication-title: J. Chromatogr. A – volume: 680 start-page: 321 year: 1994 end-page: 340 publication-title: J. Chromatogr. A – start-page: 2381 year: 2005 end-page: 2384 publication-title: Proteomics – start-page: 95 year: 2005 end-page: 106 publication-title: Mol. Cell. Endocrinol. – volume: 817 start-page: 67 year: 2005 end-page: 76 publication-title: J. Chromatogr. B – volume: 76 start-page: 3856 year: 2004 end-page: 3860 publication-title: Anal. Chem. – volume: 1 start-page: 845 year: 2002 end-page: 867 publication-title: Moll. Cell. Proteomics – volume: 27 start-page: 283 year: 2006 end-page: 303 publication-title: Electrophoresis – volume: 6 year: 2005 article-title: art. no. 179 publication-title: BMC: Bioinformatics – volume: 24 start-page: 834 year: 2003 end-page: 842 publication-title: Electrophoresis – volume: 1013 start-page: 173 year: 2003 end-page: 181 publication-title: J Chromatogr. A – volume: 70 start-page: 107 year: 2005 end-page: 112 publication-title: Meat Sci. – volume: 817 start-page: 35 year: 2005 end-page: 47 publication-title: J. Chromatogr. A – volume: 27 start-page: 142 year: 2006 end-page: 175 publication-title: Electrophoresis – volume: 26 start-page: 1254 year: 2005 end-page: 1290 publication-title: Electrophoresis – volume: 10 start-page: 35 year: 2006 end-page: 41 publication-title: Curr. Opin. Chem. Biol. – volume: 21 start-page: 5 year: 2005 end-page: 13 publication-title: Anal. Sci. – ident: e_1_2_1_3_2 doi: 10.1016/j.mce.2004.10.010 – ident: e_1_2_1_25_2 doi: 10.1016/S0021-9673(00)00585-9 – ident: e_1_2_1_26_2 doi: 10.1016/0021-9673(94)80083-9 – ident: e_1_2_1_7_2 doi: 10.1002/elps.200500527 – ident: e_1_2_1_16_2 doi: 10.1186/1471-2105-6-179 – ident: e_1_2_1_6_2 doi: 10.1002/elps.200500567 – ident: e_1_2_1_11_2 doi: 10.1016/j.jchromb.2004.07.045 – ident: e_1_2_1_15_2 doi: 10.1021/ac035375s – ident: e_1_2_1_18_2 doi: 10.1016/S0021-9673(99)00299-X – volume-title: Chromatographic Integration Methods year: 1990 ident: e_1_2_1_17_2 contributor: fullname: Dyson N. – ident: e_1_2_1_2_2 doi: 10.1074/mcp.R200007-MCP200 – ident: e_1_2_1_22_2 doi: 10.1002/jssc.200500013 – ident: e_1_2_1_4_2 doi: 10.1002/1522-2683(200109)22:17<3629::AID-ELPS3629>3.0.CO;2-O – ident: e_1_2_1_21_2 doi: 10.1016/j.meatsci.2004.12.004 – ident: e_1_2_1_8_2 doi: 10.2116/analsci.21.5 – ident: e_1_2_1_10_2 doi: 10.1016/j.cbpa.2005.12.017 – ident: e_1_2_1_14_2 doi: 10.1002/pmic.200401244 – ident: e_1_2_1_13_2 doi: 10.1016/j.jchromb.2005.12.051 – ident: e_1_2_1_19_2 doi: 10.1021/ac051067d – ident: e_1_2_1_24_2 doi: 10.1016/0021-9673(96)00151-3 – ident: e_1_2_1_20_2 doi: 10.1002/elps.200390105 – ident: e_1_2_1_12_2 doi: 10.1016/S0021-9673(03)00713-1 – ident: e_1_2_1_23_2 doi: 10.1002/elps.200500474 – volume: 817 start-page: 35 year: 2005 ident: e_1_2_1_5_2 publication-title: J. Chromatogr. A contributor: fullname: Issaq H. J. – ident: e_1_2_1_9_2 doi: 10.1002/elps.200410130 |
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Snippet | It has been demonstrated that CE‐MS is a very useful hyphenated technique for proteomic studies. However, the huge amount of data stored in a single CE‐MS run... It has been demonstrated that CE-MS is a very useful hyphenated technique for proteomic studies. However, the huge amount of data stored in a single CE-MS run... Abstract It has been demonstrated that CE‐MS is a very useful hyphenated technique for proteomic studies. However, the huge amount of data stored in a single... |
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SubjectTerms | Animals Automatic Data Processing - methods Biomarkers Cattle CE-MS Chemometric Cytochromes c - analysis Electrophoresis, Capillary - methods Horses Information Storage and Retrieval - methods Mapping Mass Spectrometry - instrumentation Mass Spectrometry - methods Peptide Mapping - instrumentation Peptide Mapping - methods Peptides Proteomics - instrumentation Proteomics - methods Rabbits Signal Processing, Computer-Assisted Trypsin - chemistry |
Title | Simplified 2-D CE-MS mapping: Analysis of proteolytic digests |
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