Applications of CRISPR technologies in transplantation

In transplantation, the ever‐increasing number of an organ's demand and long‐term graft dysfunction constitute some of the major problems. Therefore, alternative solutions to increase the quantity and quality of the organ supply for transplantation are desired. On this subject, revolutionary Cl...

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Published inAmerican journal of transplantation Vol. 20; no. 12; pp. 3285 - 3293
Main Authors Kuscu, Cem, Kuscu, Canan, Bajwa, Amandeep, Eason, James D., Maluf, Daniel, Mas, Valeria R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Limited 01.12.2020
Subjects
animal models: porcine
basic (laboratory) research/science
Blood & organ donations
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR
genomics
immunosuppression/immune modulation
molecular biology
Organ Transplantation
tolerance: chimerism
translational research/science
Transplantation
Transplants & implants
xenoantigen
xenotransplantation
molecular biology
translational research/science
tolerance: chimerism
immunosuppression/immune modulation
genomics
xenotransplantation
xenoantigen
animal models: porcine
basic (laboratory) research/science
Online AccessGet full text
ISSN1600-6135
1600-6143
1600-6143
DOI10.1111/ajt.16095

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Abstract In transplantation, the ever‐increasing number of an organ's demand and long‐term graft dysfunction constitute some of the major problems. Therefore, alternative solutions to increase the quantity and quality of the organ supply for transplantation are desired. On this subject, revolutionary Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology holds enormous potential for the scientific community with its expanding toolbox. In this minireview, we summarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applications in cellular‐ and organ‐level transplantation. The last part of this review includes future opportunities as well as the challenges in the transplantation field. This minireview of CRISPR technologies, the state‐of‐the‐art approach to genome editing, focuses on current and future applications in transplantation.
AbstractList In transplantation, the ever-increasing number of an organ's demand and long-term graft dysfunction constitute some of the major problems. Therefore, alternative solutions to increase the quantity and quality of the organ supply for transplantation are desired. On this subject, revolutionary Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology holds enormous potential for the scientific community with its expanding toolbox. In this minireview, we summarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applications in cellular- and organ-level transplantation. The last part of this review includes future opportunities as well as the challenges in the transplantation field.
In transplantation, the ever-increasing number of organ’s demand and long-term graft dysfunction constitute some of the major problems. Therefore, alternative solutions to increase the quantity and quality of the organ supply for transplantation are desired. On this subject, revolutionary CRISPR technology holds enormous potential for the scientific community with its expanding toolbox. In this minireview, we summarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applications at cellular and organ level transplantation. The last part of this review includes future opportunities as well as the challenges in the transplantation field.
In transplantation, the ever‐increasing number of an organ's demand and long‐term graft dysfunction constitute some of the major problems. Therefore, alternative solutions to increase the quantity and quality of the organ supply for transplantation are desired. On this subject, revolutionary Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology holds enormous potential for the scientific community with its expanding toolbox. In this minireview, we summarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applications in cellular‐ and organ‐level transplantation. The last part of this review includes future opportunities as well as the challenges in the transplantation field. This minireview of CRISPR technologies, the state‐of‐the‐art approach to genome editing, focuses on current and future applications in transplantation.
In transplantation, the ever-increasing number of an organ's demand and long-term graft dysfunction constitute some of the major problems. Therefore, alternative solutions to increase the quantity and quality of the organ supply for transplantation are desired. On this subject, revolutionary Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology holds enormous potential for the scientific community with its expanding toolbox. In this minireview, we summarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applications in cellular- and organ-level transplantation. The last part of this review includes future opportunities as well as the challenges in the transplantation field.In transplantation, the ever-increasing number of an organ's demand and long-term graft dysfunction constitute some of the major problems. Therefore, alternative solutions to increase the quantity and quality of the organ supply for transplantation are desired. On this subject, revolutionary Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology holds enormous potential for the scientific community with its expanding toolbox. In this minireview, we summarize the history and mechanism of CRISPR/Cas9 systems and explore its potential applications in cellular- and organ-level transplantation. The last part of this review includes future opportunities as well as the challenges in the transplantation field.
Author Kuscu, Cem
Eason, James D.
Kuscu, Canan
Maluf, Daniel
Bajwa, Amandeep
Mas, Valeria R.
AuthorAffiliation 1 Transplant Research Institute, James D. Eason Transplant Institute, Department of Surgery, School of Medicine, University of Tennessee Health Science Center, Memphis, TN
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Issue 12
Keywords molecular biology
translational research/science
tolerance: chimerism
immunosuppression/immune modulation
genomics
xenotransplantation
xenoantigen
animal models: porcine
basic (laboratory) research/science
Language English
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Snippet In transplantation, the ever‐increasing number of an organ's demand and long‐term graft dysfunction constitute some of the major problems. Therefore,...
In transplantation, the ever-increasing number of an organ's demand and long-term graft dysfunction constitute some of the major problems. Therefore,...
In transplantation, the ever-increasing number of organ’s demand and long-term graft dysfunction constitute some of the major problems. Therefore, alternative...
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SubjectTerms animal models: porcine
basic (laboratory) research/science
Blood & organ donations
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR
genomics
immunosuppression/immune modulation
molecular biology
Organ Transplantation
tolerance: chimerism
translational research/science
Transplantation
Transplants & implants
xenoantigen
xenotransplantation
Title Applications of CRISPR technologies in transplantation
URI https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fajt.16095
https://www.ncbi.nlm.nih.gov/pubmed/32484284
https://www.proquest.com/docview/2466040051
https://www.proquest.com/docview/2408822276
https://pubmed.ncbi.nlm.nih.gov/PMC8109183
Volume 20
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