Isolation of a high‐affinity Bet v 1‐specific IgG‐derived ScFv from a subject vaccinated with hypoallergenic Bet v 1 fragments

• Published in: Allergy (Copenhagen) Vol. 73; no. 7; pp. 1425 - 1435
• Main Authors: Gadermaier, E., Marth, K., Lupinek, C., Campana, R., Hofer, G., Blatt, K., Smiljkovic, D., Roder, U., Focke‐Tejkl, M., Vrtala, S., Keller, W., Valent, P., Valenta, R., Flicker, S.
• Format: Journal Article
• Language: English
• Published: Denmark Blackwell Publishing Ltd 01.07.2018; John Wiley and Sons Inc
• Subjects: Affinity; Allergens; Allergens - immunology; Allergies; allergy; Amino Acid Sequence; Antibody Specificity - immunology; Antigens, Plant - immunology; Base Sequence; Basophils - immunology; Basophils - metabolism; Bet v 1; Bet v 1 antigen; combinatorial cloning; Cross Reactions - immunology; Enzyme-linked immunosorbent assay; Epitope mapping; Epitopes - immunology; Food allergies; Gene Library; Gene mapping; Humans; IgG antibody; Immunization; Immunoglobulin E; Immunoglobulin E - immunology; Immunoglobulin G; Immunoglobulin G - chemistry; Immunoglobulin G - genetics; Immunoglobulin G - immunology; Immunoglobulin G - isolation & purification; Immunoglobulins; Immunotherapy; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Original; ORIGINAL ARTICLES; Patients; Phages; Pollen; recombinant hypoallergenic allergen derivative; Single-Chain Antibodies - chemistry; Single-Chain Antibodies - genetics; Single-Chain Antibodies - immunology; Single-Chain Antibodies - isolation & purification; Surface Plasmon Resonance; Vaccination; allergy; combinatorial cloning; recombinant hypoallergenic allergen derivative; IgG antibody; Bet v 1

Background Recombinant hypoallergenic allergen derivatives have been used in clinical immunotherapy studies, and clinical efficacy seems to be related to the induction of blocking IgG antibodies recognizing the wild‐type allergens. However, so far no treatment‐induced IgG antibodies have been charac...