Inhibitory effects of homoharringtonine on foot and mouth disease virus in vitro

• Published in: Journal of medical virology Vol. 91; no. 9; pp. 1595 - 1601
• Main Authors: Gong, Mei‐jiao, Li, Shi‐fang, Xie, Yin‐li, Zhao, Fu‐rong, Shao, Jun‐jun, Zhang, Yong‐guang, Wang, Wen‐hui, Chang, Hui‐yun
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.09.2019; John Wiley and Sons Inc
• Subjects: antiviral; Antiviral activity; Cell culture; FMDV; Foot & mouth disease; Goats; homoharringtonine; Hoof; In vivo methods and tests; Kidneys; Livestock; Replication; RNA virus; Serotypes; Sheep; Swine; viral replication; Virology; Virus attachment; Viruses; viral replication; FMDV; homoharringtonine; RNA virus; antiviral
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.25494

Foot‐and‐mouth disease (FMD) is a highly contagious disease that affects cloven‐hoof animals including cattle, swine, sheep, goats, and lots of wild species. Effectively control measures are urged needed. Here, we showed that homoharringtonine treatment exhibited a strong inhibitory effect against two different strains of FMDVs (O/MYA98/BY/2010 and A/GD/MM/2013) in swine kidney (IBRS‐2) cells. Further experiments demonstrated that homoharringtonine did not affect virus attachment or entry. Using time‐of‐addition assays, we found that the antiviral activity of homoharringtonine occurred primarily during the early stage of infection. These results demonstrated that homoharringtonine might be an effective anti‐FMDV drug. Further studies are required to explore the antiviral activity of homoharringtonine against FMDV replication in vivo. Highlight This is the first study to demonstrate the antiviral activity of homoharringtonine against FMDV, including two different serotypes of FMDV in cell culture. Homoharringtonine did not suppress the attachment and entry of FMDV into the cell. The antiviral effect of homoharringtonine occurred in the early phases of FMDV replication.