The Incorporation of Extracellular Vesicles from Mesenchymal Stromal Cells Into CD34+ Cells Increases Their Clonogenic Capacity and Bone Marrow Lodging Ability

• Published in: Stem cells (Dayton, Ohio) Vol. 37; no. 10; pp. 1357 - 1368
• Main Authors: Preciado, Silvia, Muntión, Sandra, Corchete, Luis A., Ramos, Teresa L., Torre, Ana G., Osugui, Lika, Rico, Ana, Espinosa‐Lara, Natalia, Gastaca, Irene, Díez‐Campelo, María, Cañizo, Consuelo, Sánchez‐Guijo, Fermín
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.10.2019; Oxford University Press
• Subjects: Apoptosis; Bone marrow; CD34 antigen; CD44 antigen; Cell cycle; Colonies; Confocal microscopy; Diabetes mellitus; Electron microscopy; Engraftment; Extracellular vesicles; Flow cytometry; Genes; Hematopoietic stem cells; Human performance; Immunodeficiency; Incorporation; Janus kinase; Kinases; Leukocytes (granulocytic); Lodging; Macrophages; Mesenchymal stem cells; Mesenchymal stromal cells; Mesenchyme; Microscopy; Nanoparticles; Phosphorylation; Polymerase chain reaction; Reverse transcription; Stat5 protein; Stem cell transplantation; Stem cells; Stromal cells; Translational and Clinical Research; Transplantation; Vesicles; Engraftment; Extracellular vesicles; Mesenchymal stromal cells; Stem cell transplantation; Hematopoietic stem cells

Mesenchymal stromal cells (MSC) may exert their functions by the release of extracellular vesicles (EV). Our aim was to analyze changes induced in CD34+ cells after the incorporation of MSC‐EV. MSC‐EV were characterized by flow cytometry (FC), Western blot, electron microscopy, and nanoparticle trac...