Development and validation of an LC–MS/MS quantitative method for endogenous deoxynucleoside triphosphates in cellular lysate

• Published in: Biomedical chromatography Vol. 31; no. 3; pp. np - n/a
• Main Authors: Chen, Xinhui, McAllister, Kevin J., Klein, Brandon, Bushman, Lane R., Anderson, Peter L.
• Format: Journal Article
• Language: English
• Published: England 01.03.2017
• Subjects: Chromatography, Liquid - methods; Deoxyadenine Nucleotides - analysis; Deoxycytosine Nucleotides - analysis; endogenous dNTP; intracellular; LC–MS/MS; pharmacodynamics; pharmacokinetics; Tandem Mass Spectrometry - methods; Thymine Nucleotides - analysis; pharmacokinetics; LC-MS/MS; intracellular; pharmacodynamics; endogenous dNTP

The endogenous deoxynucleoside triphosphate (dNTP) pool includes deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP) and thymidine triphosphate (TTP). The endogenous dNTP pool is regulated by complex enzymatic pathways that can be targeted by dru...