Identification and Functional Characterization of a Monofunctional Peroxisomal Enoyl-CoA Hydratase 2 That Participates in the Degradation of Even cis-Unsaturated Fatty Acids in Arabidopsis thaliana

• Published in: The Journal of biological chemistry Vol. 281; no. 47; pp. 35894 - 35903
• Main Authors: Goepfert, Simon, Hiltunen, J. Kalervo, Poirier, Yves
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.11.2006; American Society for Biochemistry and Molecular Biology
• Subjects: Allium cepa; Amino Acid Sequence; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis thaliana; Carbon - chemistry; Catalysis; Enoyl-CoA Hydratase - chemistry; Enoyl-CoA Hydratase - genetics; Fatty Acids - chemistry; Fatty Acids, Unsaturated - chemistry; Molecular Sequence Data; Onions; Oxygen - chemistry; Peroxisomes - chemistry; Peroxisomes - enzymology; Plant Proteins - chemistry; Recombinant Fusion Proteins - chemistry; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Sequence Homology, Amino Acid

A gene, named AtECH2, has been identified in Arabidopsis thaliana to encode a monofunctional peroxisomal enoyl-CoA hydratase 2. Homologues of AtECH2 are present in several angiosperms belonging to the Monocotyledon and Dicotyledon classes, as well as in a gymnosperm. In vitro enzyme assays demonstra...