CDC68, a yeast gene that affects regulation of cell proliferation and transcription, encodes a protein with a highly acidic carboxyl terminus

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Published inMolecular and Cellular Biology Vol. 11; no. 11; pp. 5718 - 5726
Main Authors Rowley, A, Singer, R A, Johnston, G C
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.11.1991
Subjects
Amino Acid Sequence
Base Sequence
Blotting, Northern
Cell Cycle - genetics
Cell Cycle Proteins
cell proliferation
Cyclins - genetics
DNA, Fungal - genetics
Fungal Proteins - genetics
genes
Genes, Fungal
Genotype
Heat-Shock Proteins - genetics
Kinetics
Molecular Sequence Data
Molecular Weight
Phenotype
Restriction Mapping
RNA, Fungal - genetics
RNA, Fungal - isolation & purification
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae Proteins
Transcription Factors
Transcription, Genetic
Transcriptional Elongation Factors
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Abstract Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue MCB About MCB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy MCB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0270-7306 Online ISSN: 1098-5549 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to MCB .asm.org, visit: MCB       
AbstractList The cell cycle of the budding yeast Saccharomyces cerevisiae has been investigated through the study of conditional cdc mutations that specifically affect cell cycle performance. Cells bearing the cdc68-1 mutation (J. A. Prendergast, L. E. Murray, A. Rowley, D. R. Carruthers, R. A. Singer, and G. C. Johnston, Genetics 124:81-90, 1990) are temperature sensitive for the performance of the G1 regulatory event, START. Here we describe the CDC68 gene and present evidence that the CDC68 gene product functions in transcription. CDC68 encodes a 1,035-amino-acid protein with a highly acidic and serine-rich carboxyl terminus. The abundance of transcripts from several unrelated genes is decreased in cdc68-1 mutant cells after transfer to the restrictive temperature, while at least one transcript, from the HSP82 gene, persists in an aberrant fashion. Thus, the cdc68-1 mutation has both positive and negative effects on gene expression. Our findings complement those of Malone et al. (E. A. Malone, C. D. Clark, A. Chiang, and F. Winston, Mol. Cell. Biol. 11:5710-5717, 1991), who have independently identified the CDC68 gene (as SPT16) as a transcriptional suppressor of delta-insertion mutations. Among transcripts that rapidly become depleted in cdc68-1 mutant cells are those of the G1 cyclin genes CLN1, CLN2, and CLN3/WHI1/DAF1, whose activity has been previously shown to be required for the performance of START. The decreased abundance of cyclin transcripts in cdc68-1 mutant cells, coupled with the suppression of cdc68-1-mediated START arrest by the CLN2-1 hyperactive allele of CLN2, shows that the CDC68 gene affects START through cyclin gene expression.
The cell cycle of the budding yeast Saccharomyces cerevisiae has been investigated through the study of conditional cdc mutations that specifically affect cell cycle performance. We describe the CDC68 gene and present evidence that the CDC68 gene product functions in transcription. CDC68 encodes a 1,035-amino-acid protein with a highly acidic and serine-rich carboxyl terminus. Our findings complement those of Malone et al., who have independently identified the CDC68 gene (as SPT16) as a transcriptional suppressor of delta -insertion mutations.
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Author A Rowley
R A Singer
G C Johnston
AuthorAffiliation Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, Canada
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SubjectTerms Amino Acid Sequence
Base Sequence
Blotting, Northern
Cell Cycle - genetics
Cell Cycle Proteins
cell proliferation
Cyclins - genetics
DNA, Fungal - genetics
Fungal Proteins - genetics
genes
Genes, Fungal
Genotype
Heat-Shock Proteins - genetics
Kinetics
Molecular Sequence Data
Molecular Weight
Phenotype
Restriction Mapping
RNA, Fungal - genetics
RNA, Fungal - isolation & purification
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae Proteins
Transcription Factors
Transcription, Genetic
Transcriptional Elongation Factors
Title CDC68, a yeast gene that affects regulation of cell proliferation and transcription, encodes a protein with a highly acidic carboxyl terminus
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