Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor

The earliest molecular events in T-cell recognition have not yet been fully described, and the initial T-cell receptor (TCR)-triggering mechanism remains a subject of controversy. Here, using total internal reflection/Forster resonance energy transfer microscopy, we observe a two-stage interaction b...

Full description

Saved in:
Bibliographic Details
Published inNature communications Vol. 5; no. 1; p. 5624
Main Authors Casas, Javier, Brzostek, Joanna, Zarnitsyna, Veronika I., Hong, Jin-sung, Wei, Qianru, Hoerter, John A. H., Fu, Guo, Ampudia, Jeanette, Zamoyska, Rose, Zhu, Cheng, Gascoigne, Nicholas R. J.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 27.11.2014
Nature Publishing Group
Subjects
13/109
13/95
14/33
14/35
14/63
631/250/1619/554/1775
631/250/21/324
82/29
82/83
96/31
96/33
96/35
Animals
CD3 Complex - genetics
CD3 Complex - metabolism
CD8 Antigens - genetics
CD8 Antigens - metabolism
Energy transfer
Female
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Humanities and Social Sciences
Ligands
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - genetics
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - metabolism
Major Histocompatibility Complex
Male
Mice
Mice, Knockout
multidisciplinary
Protein Binding
Receptors, Antigen, T-Cell - genetics
Receptors, Antigen, T-Cell - metabolism
Science
Science (multidisciplinary)
Synapses - enzymology
Synapses - genetics
Synapses - metabolism
T-Lymphocytes - metabolism
Online AccessGet full text
ISSN2041-1723
2041-1723
DOI10.1038/ncomms6624

Cover

Abstract The earliest molecular events in T-cell recognition have not yet been fully described, and the initial T-cell receptor (TCR)-triggering mechanism remains a subject of controversy. Here, using total internal reflection/Forster resonance energy transfer microscopy, we observe a two-stage interaction between TCR, CD8 and major histocompatibility complex (MHC)-peptide. There is an early (within seconds) interaction between CD3ζ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3ζ–CD8 interactions require CD8–MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR-triggering event is induced by free Lck. The early signalling events that trigger initial T-cell receptor signalling are not clearly defined. Here the authors show that this occurs in two stages, the first between the CD8 coreceptor and CD3 requiring Lck association to CD8, while the second interaction requires binding of major histocompatibility molecules.
AbstractList The earliest molecular events in T-cell recognition have not yet been fully described, and the initial T-cell receptor (TCR)-triggering mechanism remains a subject of controversy. Here, using total internal reflection/Forster resonance energy transfer microscopy, we observe a two-stage interaction between TCR, CD8 and major histocompatibility complex (MHC)-peptide. There is an early (within seconds) interaction between CD3ζ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3ζ-CD8 interactions require CD8-MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR-triggering event is induced by free Lck.
The earliest molecular events in T-cell recognition have not yet been fully described, and the initial T-cell receptor (TCR)-triggering mechanism remains a subject of controversy. Here, using total internal reflection/Forster resonance energy transfer microscopy, we observe a two-stage interaction between TCR, CD8 and major histocompatibility complex (MHC)-peptide. There is an early (within seconds) interaction between CD3ζ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3ζ–CD8 interactions require CD8–MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR-triggering event is induced by free Lck. The early signalling events that trigger initial T-cell receptor signalling are not clearly defined. Here the authors show that this occurs in two stages, the first between the CD8 coreceptor and CD3 requiring Lck association to CD8, while the second interaction requires binding of major histocompatibility molecules.
The earliest molecular events in T-cell recognition have not yet been fully described, and the initial T-cell receptor (TCR)-triggering mechanism remains a subject of controversy. Here, using total internal reflection/Forster resonance energy transfer microscopy, we observe a two-stage interaction between TCR, CD8 and major histocompatibility complex (MHC)-peptide. There is an early (within seconds) interaction between CD3ζ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3ζ-CD8 interactions require CD8-MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR-triggering event is induced by free Lck.The earliest molecular events in T-cell recognition have not yet been fully described, and the initial T-cell receptor (TCR)-triggering mechanism remains a subject of controversy. Here, using total internal reflection/Forster resonance energy transfer microscopy, we observe a two-stage interaction between TCR, CD8 and major histocompatibility complex (MHC)-peptide. There is an early (within seconds) interaction between CD3ζ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3ζ-CD8 interactions require CD8-MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR-triggering event is induced by free Lck.
The earliest molecular events in T cell recognition have not yet been fully described, and the initial T cell receptor (TCR) triggering mechanism remains a subject of controversy. Here, using TIRF/FRET microscopy, we observe a two-stage interaction between TCR, CD8, and MHCp. There is an early (within seconds) interaction between CD3ζ and the coreceptor CD8 that is independent of the binding of CD8 to MHC, but that requires CD8 association with Lck. Later (several minutes) CD3ζ-CD8 interactions require CD8-MHC binding. Lck can be found free or bound to the coreceptor. This work indicates that the initial TCR triggering event is induced by free Lck.
ArticleNumber 5624
Author Gascoigne, Nicholas R. J.
Zarnitsyna, Veronika I.
Zamoyska, Rose
Brzostek, Joanna
Hoerter, John A. H.
Fu, Guo
Zhu, Cheng
Casas, Javier
Wei, Qianru
Hong, Jin-sung
Ampudia, Jeanette
AuthorAffiliation 1 Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore 117545
2 Department of Immunology and Microbial Sciences, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA, 92037 USA
3 Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
4 George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
5 Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, Scotland, UK
AuthorAffiliation_xml – name: 1 Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore 117545
– name: 5 Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, Scotland, UK
– name: 3 Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
– name: 2 Department of Immunology and Microbial Sciences, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA, 92037 USA
– name: 4 George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Author_xml – sequence: 1
  givenname: Javier
  surname: Casas
  fullname: Casas, Javier
  organization: Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Department of Immunology and Microbial Sciences, The Scripps Research Institute
– sequence: 2
  givenname: Joanna
  surname: Brzostek
  fullname: Brzostek, Joanna
  organization: Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Department of Immunology and Microbial Sciences, The Scripps Research Institute
– sequence: 3
  givenname: Veronika I.
  surname: Zarnitsyna
  fullname: Zarnitsyna, Veronika I.
  organization: Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Present address: Department of Biology, Emory University, Atlanta, Georgia 30332, USA
– sequence: 4
  givenname: Jin-sung
  surname: Hong
  fullname: Hong, Jin-sung
  organization: George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology and Emory University
– sequence: 5
  givenname: Qianru
  surname: Wei
  fullname: Wei, Qianru
  organization: Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore
– sequence: 6
  givenname: John A. H.
  surname: Hoerter
  fullname: Hoerter, John A. H.
  organization: Department of Immunology and Microbial Sciences, The Scripps Research Institute, Present address: Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA
– sequence: 7
  givenname: Guo
  surname: Fu
  fullname: Fu, Guo
  organization: Department of Immunology and Microbial Sciences, The Scripps Research Institute, Present address: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Fujian 361102, China
– sequence: 8
  givenname: Jeanette
  surname: Ampudia
  fullname: Ampudia, Jeanette
  organization: Department of Immunology and Microbial Sciences, The Scripps Research Institute, Present address: Takeda Pharmaceutical Company, San Diego, California 92121, USA
– sequence: 9
  givenname: Rose
  surname: Zamoyska
  fullname: Zamoyska, Rose
  organization: Institute of Immunology and Infection Research, University of Edinburgh
– sequence: 10
  givenname: Cheng
  surname: Zhu
  fullname: Zhu, Cheng
  organization: Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology and Emory University
– sequence: 11
  givenname: Nicholas R. J.
  surname: Gascoigne
  fullname: Gascoigne, Nicholas R. J.
  email: micnrjg@nus.edu.sg
  organization: Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Department of Immunology and Microbial Sciences, The Scripps Research Institute
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25427562$$D View this record in MEDLINE/PubMed
BookMark eNptkVtv1DAQhS1URC_0hR-AIvUFgQIZ27m9IKGlhUorIaHybE2cSeo2sbe2F9R_j7fbwlI6Lx6Nvzk69jlke9ZZYuwVFO-hEM0Hq908h6ri8hk74IWEHGou9nb6fXYcwlWRSrTQSPmC7fNS8rqs-AE7X5oRbZ-THXGkPrtYfM9MyKI340g-DbrbbKmvM-tihiE4bTCm6S8TL7PF5ybTzpOmVXT-JXs-4BTo-P48Yj_OTi8WX_Plty_ni0_LXEvJY056qLtOdiX1UJGEHkrUKIehgpqEBiCNAyAg9roVUNRtwUtCjqIUSaETR-zjVne17mbqNdnocVIrb2b0t8qhUf_eWHOpRvdTSS4bLtok8OZewLubNYWoZhM0TRNacuugoOJN0wBUkNCTR-iVW3ubnndHcSlauaFe7zr6Y-XhlxNQbAHtXQieBqVNxGjcxqCZFBRqk6X6m2Vaefto5UH1SfjdFg4Jsim3HZv_078BMk2vdQ
CitedBy_id crossref_primary_10_1038_s41590_022_01366_0
crossref_primary_10_1073_pnas_1600163113
crossref_primary_10_1093_gpbjnl_qzae060
crossref_primary_10_3389_fimmu_2015_00582
crossref_primary_10_1126_sciadv_aaz3223
crossref_primary_10_1073_pnas_1607436113
crossref_primary_10_1016_j_celrep_2021_109375
crossref_primary_10_3389_fimmu_2022_1024931
crossref_primary_10_4049_jimmunol_1501407
crossref_primary_10_1038_s41419_018_0528_7
crossref_primary_10_1091_mbc_e17_04_0228
crossref_primary_10_1360_nso_20230087
crossref_primary_10_3389_fcell_2020_597627
crossref_primary_10_3389_fimmu_2021_658611
crossref_primary_10_1126_sciadv_aau2001
crossref_primary_10_1073_pnas_1913334117
crossref_primary_10_1038_s41423_020_0470_3
crossref_primary_10_3389_fimmu_2024_1392933
crossref_primary_10_1038_s41598_017_13793_3
crossref_primary_10_1126_science_abe9124
crossref_primary_10_1038_s41467_021_22965_9
crossref_primary_10_1016_j_isci_2023_105954
crossref_primary_10_1016_j_intimp_2023_110154
crossref_primary_10_1146_annurev_cellbio_111315_125324
crossref_primary_10_3389_fimmu_2021_721722
crossref_primary_10_1038_s41392_021_00823_w
crossref_primary_10_1016_j_bpj_2020_01_041
crossref_primary_10_4049_jimmunol_1600037
crossref_primary_10_1038_s41467_022_34587_w
crossref_primary_10_3389_fimmu_2024_1503018
crossref_primary_10_3389_fphys_2023_1321996
crossref_primary_10_1007_s12026_020_09148_2
crossref_primary_10_3390_ijms232214255
crossref_primary_10_3389_fimmu_2024_1343575
crossref_primary_10_3390_ijms20143500
crossref_primary_10_3389_fimmu_2018_00713
crossref_primary_10_1126_sciimmunol_adp5016
crossref_primary_10_3389_fimmu_2021_673196
crossref_primary_10_3390_cells10040834
crossref_primary_10_1242_jcs_258046
crossref_primary_10_4049_jimmunol_2101014
crossref_primary_10_3390_ijms21197424
crossref_primary_10_1038_s41590_022_01392_y
crossref_primary_10_1146_annurev_immunol_032414_112158
crossref_primary_10_1016_j_str_2023_11_013
crossref_primary_10_1073_pnas_2213538119
crossref_primary_10_1073_pnas_1701990114
crossref_primary_10_7554_eLife_88225_3
crossref_primary_10_1016_j_celrep_2016_12_057
crossref_primary_10_1016_j_it_2015_03_004
crossref_primary_10_1038_s41590_020_0732_3
crossref_primary_10_1111_imm_12809
crossref_primary_10_7554_eLife_88225
crossref_primary_10_1016_j_tips_2015_11_004
crossref_primary_10_1016_j_pbiomolbio_2015_04_004
Cites_doi 10.1034/j.1600-065X.2003.00015.x
10.1038/384484a0
10.1073/pnas.1010568107
10.1016/0092-8674(93)90511-N
10.4049/jimmunol.179.11.7653
10.1038/342278a0
10.1016/S0006-3495(98)74074-3
10.1016/j.immuni.2010.05.011
10.1126/science.290.5489.127
10.1002/eji.1830260920
10.1016/S0022-1759(03)00193-5
10.1038/nri2397
10.1016/j.immuni.2011.01.001
10.1038/sj.onc.1208074
10.1111/j.1600-065X.2008.00745.x
10.4049/jimmunol.0802639
10.1016/S1074-7613(02)00481-8
10.1073/pnas.1016388108
10.1126/science.1085643
10.4049/jimmunol.180.12.8211
10.1016/S1074-7613(03)00019-0
10.1016/j.immuni.2010.12.017
10.1074/jbc.M500555200
10.1016/j.immuni.2007.05.017
10.4049/jimmunol.177.6.3930
10.1002/cyto.a.20747
10.1084/jem.183.3.1053
10.1126/scisignal.2003607
10.1126/science.7509083
10.4049/jimmunol.177.9.6007
10.1084/jem.189.2.253
10.1074/jbc.271.40.25003
10.1016/S1074-7613(00)80205-8
10.1038/ni1220
10.1016/j.coi.2003.11.008
10.1117/1.JBO.17.1.011002
10.1529/biophysj.103.022087
10.1146/annurev-immunol-030409-101308
10.1038/embor.2011.209
10.1084/jem.20122528
10.1038/sj.onc.1208081
10.1084/jem.178.1.245
10.1016/S1074-7613(02)00301-1
10.1016/j.coi.2004.05.019
10.3389/fimmu.2011.00072
10.1111/j.1600-065X.2008.00753.x
10.1084/jem.181.6.1997
10.1126/science.285.5425.221
10.1034/j.1600-065X.2003.00003.x
10.1016/j.immuni.2006.05.015
ContentType Journal Article
Copyright Springer Nature Limited 2014
Copyright Nature Publishing Group Nov 2014
Copyright_xml – notice: Springer Nature Limited 2014
– notice: Copyright Nature Publishing Group Nov 2014
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
3V.
7QL
7QP
7QR
7SN
7SS
7ST
7T5
7T7
7TM
7TO
7X7
7XB
88E
8AO
8FD
8FE
8FG
8FH
8FI
8FJ
8FK
ABUWG
AEUYN
AFKRA
ARAPS
AZQEC
BBNVY
BENPR
BGLVJ
BHPHI
C1K
CCPQU
DWQXO
FR3
FYUFA
GHDGH
GNUQQ
H94
HCIFZ
K9.
LK8
M0S
M1P
M7P
P5Z
P62
P64
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
RC3
SOI
7X8
5PM
DOI 10.1038/ncomms6624
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
ProQuest Central (Corporate)
Bacteriology Abstracts (Microbiology B)
Calcium & Calcified Tissue Abstracts
Chemoreception Abstracts
Ecology Abstracts
Entomology Abstracts (Full archive)
Environment Abstracts
Immunology Abstracts
Industrial and Applied Microbiology Abstracts (Microbiology A)
Nucleic Acids Abstracts
Oncogenes and Growth Factors Abstracts
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
ProQuest Pharma Collection
Technology Research Database
ProQuest SciTech Collection
ProQuest Technology Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest One Sustainability
ProQuest Central UK/Ireland
Advanced Technologies & Aerospace Collection
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Technology Collection
Natural Science Collection
Environmental Sciences and Pollution Management
ProQuest One
ProQuest Central
Engineering Research Database
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
AIDS and Cancer Research Abstracts
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Biological Sciences
ProQuest Health & Medical Collection
Medical Database
Biological Science Database
Advanced Technologies & Aerospace Database
ProQuest Advanced Technologies & Aerospace Collection
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
Genetics Abstracts
Environment Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Publicly Available Content Database
ProQuest Central Student
Oncogenes and Growth Factors Abstracts
ProQuest Advanced Technologies & Aerospace Collection
ProQuest Central Essentials
Nucleic Acids Abstracts
SciTech Premium Collection
ProQuest Central China
Environmental Sciences and Pollution Management
ProQuest One Applied & Life Sciences
ProQuest One Sustainability
Health Research Premium Collection
Natural Science Collection
Health & Medical Research Collection
Biological Science Collection
Chemoreception Abstracts
Industrial and Applied Microbiology Abstracts (Microbiology A)
ProQuest Central (New)
ProQuest Medical Library (Alumni)
Advanced Technologies & Aerospace Collection
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
ProQuest Technology Collection
Health Research Premium Collection (Alumni)
Biological Science Database
Ecology Abstracts
ProQuest Hospital Collection (Alumni)
Biotechnology and BioEngineering Abstracts
Entomology Abstracts
ProQuest Health & Medical Complete
ProQuest One Academic UKI Edition
Engineering Research Database
ProQuest One Academic
Calcium & Calcified Tissue Abstracts
ProQuest One Academic (New)
Technology Collection
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Pharma Collection
ProQuest Central
ProQuest Health & Medical Research Collection
Genetics Abstracts
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
Bacteriology Abstracts (Microbiology B)
AIDS and Cancer Research Abstracts
ProQuest SciTech Collection
Advanced Technologies & Aerospace Database
ProQuest Medical Library
Immunology Abstracts
Environment Abstracts
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList MEDLINE
Publicly Available Content Database

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 3
  dbid: 8FG
  name: ProQuest Technology Collection
  url: https://search.proquest.com/technologycollection1
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2041-1723
EndPage 5624
ExternalDocumentID PMC4248239
3509017341
25427562
10_1038_ncomms6624
Genre Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NIAID NIH HHS
  grantid: R29 AI038282
– fundername: NIGMS NIH HHS
  grantid: R01 GM096187
– fundername: NIAID NIH HHS
  grantid: R01 AI038282
– fundername: NIAID NIH HHS
  grantid: T32AI07244
– fundername: NIAID NIH HHS
  grantid: T32 AI007244
– fundername: Wellcome Trust
  grantid: 095831
– fundername: NIAID NIH HHS
  grantid: AI038282
– fundername: Wellcome Trust
  grantid: 096669
– fundername: NIAID NIH HHS
  grantid: R56 AI038282
– fundername: NIGMS NIH HHS
  grantid: GM96187
GroupedDBID ---
0R~
39C
3V.
4.4
53G
70F
7X7
88E
8AO
8FE
8FG
8FH
8FI
8FJ
AAHBH
AAJSJ
ABAWZ
ABUWG
ACGFO
ACGFS
ACIWK
ACMJI
ACPRK
ACSMW
ADBBV
ADFRT
ADMLS
ADRAZ
AENEX
AEUYN
AFKRA
AFRAH
AHMBA
AJTQC
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AMTXH
AOIJS
ARAPS
ASPBG
AVWKF
AZFZN
BAPOH
BBNVY
BCNDV
BENPR
BGLVJ
BHPHI
BPHCQ
BVXVI
C6C
CCPQU
DIK
EBLON
EBS
EE.
EJD
EMOBN
F5P
FEDTE
FYUFA
GROUPED_DOAJ
HCIFZ
HMCUK
HVGLF
HYE
HZ~
LK8
M1P
M48
M7P
M~E
NAO
O9-
OK1
P2P
P62
PIMPY
PQQKQ
PROAC
PSQYO
RNS
RNT
RNTTT
RPM
SNYQT
SV3
TSG
UKHRP
AASML
AAYXX
CITATION
PHGZM
PHGZT
CGR
CUY
CVF
ECM
EIF
NPM
PJZUB
PPXIY
PQGLB
7QL
7QP
7QR
7SN
7SS
7ST
7T5
7T7
7TM
7TO
7XB
8FD
8FK
AARCD
AZQEC
C1K
DWQXO
FR3
GNUQQ
H94
K9.
P64
PKEHL
PQEST
PQUKI
PRINS
RC3
SOI
7X8
5PM
ID FETCH-LOGICAL-c442t-ecf7bb4b5ed16e41d15aca4ff617e3c11ecaf1a1aadc931079025ea2a353c44b3
IEDL.DBID M48
ISSN 2041-1723
IngestDate Thu Aug 21 13:21:00 EDT 2025
Fri Jul 11 06:36:53 EDT 2025
Wed Aug 13 04:08:37 EDT 2025
Mon Jul 21 06:03:03 EDT 2025
Tue Jul 01 02:30:47 EDT 2025
Thu Apr 24 23:03:34 EDT 2025
Fri Feb 21 02:37:40 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
License Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c442t-ecf7bb4b5ed16e41d15aca4ff617e3c11ecaf1a1aadc931079025ea2a353c44b3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
content type line 14
ObjectType-Feature-2
content type line 23
Present addresses: V.I.Z., Department of Biology, Emory University, Atlanta, GA; J.A.H.H., Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA; G.F., State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Fujian 361102, China; J.A., Takeda Pharmaceutical Company, San Diego, CA 92121, USA.
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.1038/ncomms6624
PMID 25427562
PQID 1628243941
PQPubID 546298
PageCount 1
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_4248239
proquest_miscellaneous_1628881161
proquest_journals_1628243941
pubmed_primary_25427562
crossref_citationtrail_10_1038_ncomms6624
crossref_primary_10_1038_ncomms6624
springer_journals_10_1038_ncomms6624
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20141127
PublicationDateYYYYMMDD 2014-11-27
PublicationDate_xml – month: 11
  year: 2014
  text: 20141127
  day: 27
PublicationDecade 2010
PublicationPlace London
PublicationPlace_xml – name: London
– name: England
PublicationTitle Nature communications
PublicationTitleAbbrev Nat Commun
PublicationTitleAlternate Nat Commun
PublicationYear 2014
Publisher Nature Publishing Group UK
Nature Publishing Group
Publisher_xml – name: Nature Publishing Group UK
– name: Nature Publishing Group
References Zal, Zal, Gascoigne (CR21) 2002; 16
Salmond, Filby, Qureshi, Caserta, Zamoyska (CR2) 2009; 228
Hoerter (CR27) 2013; 210
Stotz, Bolliger, Carbone, Palmer (CR49) 1999; 189
Yachi, Ampudia, Gascoigne, Zal (CR23) 2005; 6
Stirnweiss (CR46) 2013; 6
van der Merwe, Cordoba (CR17) 2011; 34
Mallaun (CR25) 2008; 180
Levin, Abraham, Anderson, Forbush, Perlmutter (CR43) 1993; 178
Gascoigne, Casas, Brzostek, Rybakin (CR3) 2011; 2
Thome, Germain, DiSanto, Acuto (CR48) 1996; 26
Fooksman (CR7) 2010; 28
Roszik, Lisboa, Szöllősi, Vereb (CR51) 2009; 75
Yachi, Ampudia, Zal, Gascoigne (CR24) 2006; 25
Xu, Littman (CR18) 1993; 74
Huse (CR8) 2007; 27
Acuto, Di Bartolo, Michel (CR1) 2008; 8
Jiang (CR9) 2011; 34
Förster (CR29) 2012; 17
Yamaguchi, Hendrickson (CR44) 1996; 384
Zamoyska (CR6) 2003; 191
Yudushkin, Vale (CR36) 2010; 107
Palacios, Weiss (CR40) 2004; 23
Groves, Dustin (CR31) 2003; 278
Zal, Gascoigne (CR30) 2004; 86
Boggon, Eck (CR12) 2004; 23
van Oers, Killeen, Weiss (CR38) 1996; 183
Zal, Gascoigne (CR50) 2004; 16
Devine (CR32) 2006; 177
Iwashima, Irving, van Oers, Chan, Weiss (CR37) 1994; 263
Wooldridge (CR11) 2005; 280
Ehrlich, Ebert, Krummel, Weiss, Davis (CR13) 2002; 17
Holler, Kranz (CR10) 2003; 18
Nika (CR15) 2010; 32
Seddon (CR42) 2000; 290
Paster (CR45) 2009; 182
Rybakin, Clamme, Ampudia, Yachi, Gascoigne (CR28) 2011; 12
Artyomov, Lis, Devadas, Davis, Chakraborty (CR4) 2010; 107
Pitcher (CR5) 2003; 191
Grakoui (CR26) 1999; 285
Huang, Edwards, Evavold, Zhu (CR34) 2007; 179
Legname (CR41) 2000; 12
Gascoigne, Zal (CR22) 2004; 16
Fragoso, Pyarajan, Irie, Burakoff (CR20) 2006; 177
Thome, Duplay, Guttinger, Acuto (CR47) 1995; 181
Kim, Sun, Blacklow, Wagner, Eck (CR14) 2003; 301
Lee-Fruman, Collins, Burakoff (CR19) 1996; 271
Chesla, Selvaraj, Zhu (CR35) 1998; 75
Rossy, Williamson, Gaus (CR16) 2012; 3
Zamoyska (CR33) 1989; 342
Au-Yeung (CR39) 2009; 228
G Legname (BFncomms6624_CR41) 2000; 12
NRJ Gascoigne (BFncomms6624_CR3) 2011; 2
RC Fragoso (BFncomms6624_CR20) 2006; 177
N Jiang (BFncomms6624_CR9) 2011; 34
DR Fooksman (BFncomms6624_CR7) 2010; 28
JAH Hoerter (BFncomms6624_CR27) 2013; 210
B Seddon (BFncomms6624_CR42) 2000; 290
T Zal (BFncomms6624_CR30) 2004; 86
T Zal (BFncomms6624_CR50) 2004; 16
H Yamaguchi (BFncomms6624_CR44) 1996; 384
M Mallaun (BFncomms6624_CR25) 2008; 180
M Iwashima (BFncomms6624_CR37) 1994; 263
T Förster (BFncomms6624_CR29) 2012; 17
NS van Oers (BFncomms6624_CR38) 1996; 183
IA Yudushkin (BFncomms6624_CR36) 2010; 107
T Zal (BFncomms6624_CR21) 2002; 16
KK Lee-Fruman (BFncomms6624_CR19) 1996; 271
J Roszik (BFncomms6624_CR51) 2009; 75
LIR Ehrlich (BFncomms6624_CR13) 2002; 17
A Grakoui (BFncomms6624_CR26) 1999; 285
SE Chesla (BFncomms6624_CR35) 1998; 75
PA van der Merwe (BFncomms6624_CR17) 2011; 34
W Paster (BFncomms6624_CR45) 2009; 182
M Thome (BFncomms6624_CR48) 1996; 26
M Huse (BFncomms6624_CR8) 2007; 27
J Rossy (BFncomms6624_CR16) 2012; 3
R Zamoyska (BFncomms6624_CR6) 2003; 191
TJ Boggon (BFncomms6624_CR12) 2004; 23
L Wooldridge (BFncomms6624_CR11) 2005; 280
K Nika (BFncomms6624_CR15) 2010; 32
MN Artyomov (BFncomms6624_CR4) 2010; 107
EH Palacios (BFncomms6624_CR40) 2004; 23
BB Au-Yeung (BFncomms6624_CR39) 2009; 228
SH Stotz (BFncomms6624_CR49) 1999; 189
PD Holler (BFncomms6624_CR10) 2003; 18
PW Kim (BFncomms6624_CR14) 2003; 301
R Zamoyska (BFncomms6624_CR33) 1989; 342
A Stirnweiss (BFncomms6624_CR46) 2013; 6
H Xu (BFncomms6624_CR18) 1993; 74
PP Yachi (BFncomms6624_CR23) 2005; 6
RJ Salmond (BFncomms6624_CR2) 2009; 228
L Devine (BFncomms6624_CR32) 2006; 177
O Acuto (BFncomms6624_CR1) 2008; 8
JT Groves (BFncomms6624_CR31) 2003; 278
NRJ Gascoigne (BFncomms6624_CR22) 2004; 16
LA Pitcher (BFncomms6624_CR5) 2003; 191
M Thome (BFncomms6624_CR47) 1995; 181
SD Levin (BFncomms6624_CR43) 1993; 178
PP Yachi (BFncomms6624_CR24) 2006; 25
J Huang (BFncomms6624_CR34) 2007; 179
V Rybakin (BFncomms6624_CR28) 2011; 12
References_xml – volume: 191
  start-page: 107
  year: 2003
  end-page: 118
  ident: CR6
  article-title: The influence of the src‐family kinases, Lck and Fyn, on T cell differentiation, survival and activation
  publication-title: Immunol. Rev.
  doi: 10.1034/j.1600-065X.2003.00015.x
– volume: 384
  start-page: 484
  year: 1996
  end-page: 489
  ident: CR44
  article-title: Structural basis for activation of human lymphocyte kinase Lck upon tyrosine phosphorylation
  publication-title: Nature
  doi: 10.1038/384484a0
– volume: 107
  start-page: 16916
  year: 2010
  end-page: 16921
  ident: CR4
  article-title: CD4 and CD8 binding to MHC molecules primarily acts to enhance Lck delivery
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.1010568107
– volume: 74
  start-page: 633
  year: 1993
  end-page: 643
  ident: CR18
  article-title: A kinase-independent function of Lck in potentiating antigen-specific T cell activation
  publication-title: Cell
  doi: 10.1016/0092-8674(93)90511-N
– volume: 179
  start-page: 7653
  year: 2007
  end-page: 7662
  ident: CR34
  article-title: Kinetics of MHC-CD8 interaction at the T cell membrane
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.179.11.7653
– volume: 342
  start-page: 278
  year: 1989
  end-page: 281
  ident: CR33
  article-title: Inability of CD8 alpha’ polypeptides to associate with p56lck correlates with impaired function in vitro and lack of expression in vivo
  publication-title: Nature
  doi: 10.1038/342278a0
– volume: 75
  start-page: 1553
  year: 1998
  end-page: 1572
  ident: CR35
  article-title: Measuring two-dimensional receptor-ligand binding kinetics by micropipette
  publication-title: Biophys. J.
  doi: 10.1016/S0006-3495(98)74074-3
– volume: 32
  start-page: 766
  year: 2010
  end-page: 777
  ident: CR15
  article-title: Constitutively active Lck kinase in T cells drives antigen receptor signal transduction
  publication-title: Immunity
  doi: 10.1016/j.immuni.2010.05.011
– volume: 290
  start-page: 127
  year: 2000
  end-page: 131
  ident: CR42
  article-title: Long-term survival but impaired homeostatic proliferation of naive T cells in the absence of p56lck
  publication-title: Science
  doi: 10.1126/science.290.5489.127
– volume: 3
  start-page: 167
  year: 2012
  ident: CR16
  article-title: How does the kinase Lck phosphorylate the T cell receptor? Spatial organization as a regulatory mechanism
  publication-title: Front. Immunol.
– volume: 26
  start-page: 2093
  year: 1996
  end-page: 2100
  ident: CR48
  article-title: The p56lck SH2 domain mediates recruitment of CD8/p56lck to the activated T cell receptor/CD3/zeta complex
  publication-title: Eur. J. Immunol.
  doi: 10.1002/eji.1830260920
– volume: 278
  start-page: 19
  year: 2003
  end-page: 32
  ident: CR31
  article-title: Supported planar bilayers in studies on immune cell adhesion and communication
  publication-title: J. Immunol. Methods
  doi: 10.1016/S0022-1759(03)00193-5
– volume: 8
  start-page: 699
  year: 2008
  end-page: 712
  ident: CR1
  article-title: Tailoring T-cell receptor signals by proximal negative feedback mechanisms
  publication-title: Nat. Rev. Immunol.
  doi: 10.1038/nri2397
– volume: 34
  start-page: 1
  year: 2011
  end-page: 3
  ident: CR17
  article-title: Late arrival: recruiting coreceptors to the T cell receptor complex
  publication-title: Immunity
  doi: 10.1016/j.immuni.2011.01.001
– volume: 23
  start-page: 7990
  year: 2004
  end-page: 8000
  ident: CR40
  article-title: Function of the Src-family kinases, Lck and Fyn, in T-cell development and activation
  publication-title: Oncogene
  doi: 10.1038/sj.onc.1208074
– volume: 228
  start-page: 9
  year: 2009
  end-page: 22
  ident: CR2
  article-title: T-cell receptor proximal signaling via the Src-family kinases, Lck and Fyn, influences T-cell activation, differentiation, and tolerance
  publication-title: Immunol. Rev.
  doi: 10.1111/j.1600-065X.2008.00745.x
– volume: 182
  start-page: 2160
  year: 2009
  end-page: 2167
  ident: CR45
  article-title: Genetically encoded Förster resonance energy transfer sensors for the conformation of the Src family kinase Lck
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.0802639
– volume: 17
  start-page: 809
  year: 2002
  end-page: 822
  ident: CR13
  article-title: Dynamics of p56lck translocation to the T cell immunological synapse following agonist and antagonist stimulation
  publication-title: Immunity
  doi: 10.1016/S1074-7613(02)00481-8
– volume: 107
  start-page: 22128
  year: 2010
  end-page: 22133
  ident: CR36
  article-title: Imaging T-cell receptor activation reveals accumulation of tyrosine-phosphorylated CD3ζ in the endosomal compartment
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.1016388108
– volume: 301
  start-page: 1725
  year: 2003
  end-page: 1728
  ident: CR14
  article-title: A zinc clasp structure tethers Lck to T cell coreceptors CD4 and CD8
  publication-title: Science
  doi: 10.1126/science.1085643
– volume: 180
  start-page: 8211
  year: 2008
  end-page: 8221
  ident: CR25
  article-title: The T cell receptor’s alpha-chain connecting peptide motif promotes close approximation of the CD8 coreceptor allowing efficient signal initiation
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.180.12.8211
– volume: 18
  start-page: 255
  year: 2003
  end-page: 264
  ident: CR10
  article-title: Quantitative analysis of the contribution of TCR/pepMHC affinity and CD8 to T cell activation
  publication-title: Immunity
  doi: 10.1016/S1074-7613(03)00019-0
– volume: 34
  start-page: 13
  year: 2011
  end-page: 23
  ident: CR9
  article-title: Two-stage cooperative T cell receptor-peptide major histocompatibility complex-CD8 trimolecular interactions amplify antigen discrimination
  publication-title: Immunity
  doi: 10.1016/j.immuni.2010.12.017
– volume: 280
  start-page: 27491
  year: 2005
  end-page: 27501
  ident: CR11
  article-title: Interaction between the CD8 coreceptor and major histocompatibility complex class I stabilizes T cell receptor-antigen complexes at the cell surface
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M500555200
– volume: 27
  start-page: 76
  year: 2007
  end-page: 88
  ident: CR8
  article-title: Spatial and temporal dynamics of T cell receptor signaling with a photoactivatable agonist
  publication-title: Immunity
  doi: 10.1016/j.immuni.2007.05.017
– volume: 177
  start-page: 3930
  year: 2006
  end-page: 3938
  ident: CR32
  article-title: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.177.6.3930
– volume: 75
  start-page: 761
  year: 2009
  end-page: 767
  ident: CR51
  article-title: Evaluation of intensity-based ratiometric FRET in image cytometry--approaches and a software solution
  publication-title: Cytometry A
  doi: 10.1002/cyto.a.20747
– volume: 183
  start-page: 1053
  year: 1996
  end-page: 1062
  ident: CR38
  article-title: Lck regulates the tyrosine phosphorylation of the T cell receptor subunits and ZAP-70 in murine thymocytes
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.183.3.1053
– volume: 6
  start-page: ra13
  year: 2013
  ident: CR46
  article-title: T cell activation results in conformational changes in the Src family kinase Lck to induce its activation
  publication-title: Sci. Signal.
  doi: 10.1126/scisignal.2003607
– volume: 263
  start-page: 1136
  year: 1994
  end-page: 1139
  ident: CR37
  article-title: Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases
  publication-title: Science
  doi: 10.1126/science.7509083
– volume: 177
  start-page: 6007
  year: 2006
  end-page: 6017
  ident: CR20
  article-title: A CD8/Lck transgene is able to drive thymocyte differentiation
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.177.9.6007
– volume: 189
  start-page: 253
  year: 1999
  end-page: 264
  ident: CR49
  article-title: T cell receptor (TCR) antagonism without a negative signal: evidence from T cell hybridomas expressing two independent TCRs
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.189.2.253
– volume: 271
  start-page: 25003
  year: 1996
  end-page: 25010
  ident: CR19
  article-title: Role of the Lck Src homology 2 and 3 domains in protein tyrosine phosphorylation
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.271.40.25003
– volume: 12
  start-page: 537
  year: 2000
  end-page: 546
  ident: CR41
  article-title: Inducible expression of a p56Lck transgene reveals a central role for Lck in the differentiation of CD4 SP thymocytes
  publication-title: Immunity
  doi: 10.1016/S1074-7613(00)80205-8
– volume: 6
  start-page: 785
  year: 2005
  end-page: 792
  ident: CR23
  article-title: Nonstimulatory peptides contribute to antigen-induced CD8–T cell receptor interaction at the immunological synapse
  publication-title: Nat. Immunol.
  doi: 10.1038/ni1220
– volume: 16
  start-page: 114
  year: 2004
  end-page: 119
  ident: CR22
  article-title: Molecular interactions at the T cell-antigen-presenting cell interface
  publication-title: Curr. Opin. Immunol.
  doi: 10.1016/j.coi.2003.11.008
– volume: 17
  start-page: 011002
  year: 2012
  ident: CR29
  article-title: Energy migration and fluorescence. 1946
  publication-title: J. Biomed. Opt.
  doi: 10.1117/1.JBO.17.1.011002
– volume: 86
  start-page: 3923
  year: 2004
  end-page: 3939
  ident: CR30
  article-title: Photobleaching-corrected FRET efficiency imaging of live cells
  publication-title: Biophys. J.
  doi: 10.1529/biophysj.103.022087
– volume: 28
  start-page: 79
  year: 2010
  end-page: 105
  ident: CR7
  article-title: Functional anatomy of T cell activation and synapse formation
  publication-title: Annu. Rev. Immunol.
  doi: 10.1146/annurev-immunol-030409-101308
– volume: 12
  start-page: 1251
  year: 2011
  end-page: 1256
  ident: CR28
  article-title: CD8αα and -αβ isotypes are equally recruited to the immunological synapse through their ability to bind to MHC class I
  publication-title: EMBO Rep.
  doi: 10.1038/embor.2011.209
– volume: 210
  start-page: 1807
  year: 2013
  end-page: 1821
  ident: CR27
  article-title: Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.20122528
– volume: 23
  start-page: 7918
  year: 2004
  end-page: 7927
  ident: CR12
  article-title: Structure and regulation of Src family kinases
  publication-title: Oncogene
  doi: 10.1038/sj.onc.1208081
– volume: 178
  start-page: 245
  year: 1993
  end-page: 255
  ident: CR43
  article-title: The protein tyrosine kinase p56lck regulates thymocyte development independently of its interaction with CD4 and CD8 coreceptors [corrected]
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.178.1.245
– volume: 16
  start-page: 521
  year: 2002
  end-page: 534
  ident: CR21
  article-title: Inhibition of T cell receptor-coreceptor interactions by antagonist ligands visualized by live FRET imaging of the T-hybridoma immunological synapse
  publication-title: Immunity
  doi: 10.1016/S1074-7613(02)00301-1
– volume: 16
  start-page: 418
  year: 2004
  end-page: 427
  ident: CR50
  article-title: Using live FRET imaging to reveal early protein-protein interactions during T cell activation
  publication-title: Curr. Opin. Immunol.
  doi: 10.1016/j.coi.2004.05.019
– volume: 2
  start-page: 72
  year: 2011
  ident: CR3
  article-title: Initiation of TCR phosphorylation and signal transduction
  publication-title: Front. Immunol.
  doi: 10.3389/fimmu.2011.00072
– volume: 228
  start-page: 41
  year: 2009
  end-page: 57
  ident: CR39
  article-title: The structure, regulation, and function of ZAP-70
  publication-title: Immunol. Rev.
  doi: 10.1111/j.1600-065X.2008.00753.x
– volume: 181
  start-page: 1997
  year: 1995
  end-page: 2006
  ident: CR47
  article-title: Syk and ZAP-70 mediate recruitment of p56lck/CD4 to the activated T cell receptor/CD3/zeta complex
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.181.6.1997
– volume: 285
  start-page: 221
  year: 1999
  end-page: 227
  ident: CR26
  article-title: The immunological synapse: a molecular machine controlling T cell activation
  publication-title: Science
  doi: 10.1126/science.285.5425.221
– volume: 191
  start-page: 47
  year: 2003
  end-page: 61
  ident: CR5
  article-title: The formation and functions of the 21‐ and 23‐kDa tyrosine‐phosphorylated TCR zeta subunits
  publication-title: Immunol. Rev.
  doi: 10.1034/j.1600-065X.2003.00003.x
– volume: 25
  start-page: 203
  year: 2006
  end-page: 211
  ident: CR24
  article-title: Altered peptide ligands induce delayed CD8-T cell receptor interaction—a role for CD8 in distinguishing antigen quality
  publication-title: Immunity
  doi: 10.1016/j.immuni.2006.05.015
– volume: 86
  start-page: 3923
  year: 2004
  ident: BFncomms6624_CR30
  publication-title: Biophys. J.
  doi: 10.1529/biophysj.103.022087
– volume: 18
  start-page: 255
  year: 2003
  ident: BFncomms6624_CR10
  publication-title: Immunity
  doi: 10.1016/S1074-7613(03)00019-0
– volume: 290
  start-page: 127
  year: 2000
  ident: BFncomms6624_CR42
  publication-title: Science
  doi: 10.1126/science.290.5489.127
– volume: 17
  start-page: 809
  year: 2002
  ident: BFncomms6624_CR13
  publication-title: Immunity
  doi: 10.1016/S1074-7613(02)00481-8
– volume: 16
  start-page: 521
  year: 2002
  ident: BFncomms6624_CR21
  publication-title: Immunity
  doi: 10.1016/S1074-7613(02)00301-1
– volume: 6
  start-page: ra13
  year: 2013
  ident: BFncomms6624_CR46
  publication-title: Sci. Signal.
  doi: 10.1126/scisignal.2003607
– volume: 12
  start-page: 1251
  year: 2011
  ident: BFncomms6624_CR28
  publication-title: EMBO Rep.
  doi: 10.1038/embor.2011.209
– volume: 2
  start-page: 72
  year: 2011
  ident: BFncomms6624_CR3
  publication-title: Front. Immunol.
  doi: 10.3389/fimmu.2011.00072
– volume: 74
  start-page: 633
  year: 1993
  ident: BFncomms6624_CR18
  publication-title: Cell
  doi: 10.1016/0092-8674(93)90511-N
– volume: 12
  start-page: 537
  year: 2000
  ident: BFncomms6624_CR41
  publication-title: Immunity
  doi: 10.1016/S1074-7613(00)80205-8
– volume: 25
  start-page: 203
  year: 2006
  ident: BFncomms6624_CR24
  publication-title: Immunity
  doi: 10.1016/j.immuni.2006.05.015
– volume: 182
  start-page: 2160
  year: 2009
  ident: BFncomms6624_CR45
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.0802639
– volume: 107
  start-page: 16916
  year: 2010
  ident: BFncomms6624_CR4
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.1010568107
– volume: 178
  start-page: 245
  year: 1993
  ident: BFncomms6624_CR43
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.178.1.245
– volume: 228
  start-page: 41
  year: 2009
  ident: BFncomms6624_CR39
  publication-title: Immunol. Rev.
  doi: 10.1111/j.1600-065X.2008.00753.x
– volume: 8
  start-page: 699
  year: 2008
  ident: BFncomms6624_CR1
  publication-title: Nat. Rev. Immunol.
  doi: 10.1038/nri2397
– volume: 278
  start-page: 19
  year: 2003
  ident: BFncomms6624_CR31
  publication-title: J. Immunol. Methods
  doi: 10.1016/S0022-1759(03)00193-5
– volume: 191
  start-page: 107
  year: 2003
  ident: BFncomms6624_CR6
  publication-title: Immunol. Rev.
  doi: 10.1034/j.1600-065X.2003.00015.x
– volume: 34
  start-page: 13
  year: 2011
  ident: BFncomms6624_CR9
  publication-title: Immunity
  doi: 10.1016/j.immuni.2010.12.017
– volume: 3
  start-page: 167
  year: 2012
  ident: BFncomms6624_CR16
  publication-title: Front. Immunol.
– volume: 280
  start-page: 27491
  year: 2005
  ident: BFncomms6624_CR11
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M500555200
– volume: 181
  start-page: 1997
  year: 1995
  ident: BFncomms6624_CR47
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.181.6.1997
– volume: 191
  start-page: 47
  year: 2003
  ident: BFncomms6624_CR5
  publication-title: Immunol. Rev.
  doi: 10.1034/j.1600-065X.2003.00003.x
– volume: 17
  start-page: 011002
  year: 2012
  ident: BFncomms6624_CR29
  publication-title: J. Biomed. Opt.
  doi: 10.1117/1.JBO.17.1.011002
– volume: 342
  start-page: 278
  year: 1989
  ident: BFncomms6624_CR33
  publication-title: Nature
  doi: 10.1038/342278a0
– volume: 180
  start-page: 8211
  year: 2008
  ident: BFncomms6624_CR25
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.180.12.8211
– volume: 384
  start-page: 484
  year: 1996
  ident: BFncomms6624_CR44
  publication-title: Nature
  doi: 10.1038/384484a0
– volume: 27
  start-page: 76
  year: 2007
  ident: BFncomms6624_CR8
  publication-title: Immunity
  doi: 10.1016/j.immuni.2007.05.017
– volume: 177
  start-page: 6007
  year: 2006
  ident: BFncomms6624_CR20
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.177.9.6007
– volume: 107
  start-page: 22128
  year: 2010
  ident: BFncomms6624_CR36
  publication-title: Proc. Natl Acad. Sci. USA
  doi: 10.1073/pnas.1016388108
– volume: 263
  start-page: 1136
  year: 1994
  ident: BFncomms6624_CR37
  publication-title: Science
  doi: 10.1126/science.7509083
– volume: 183
  start-page: 1053
  year: 1996
  ident: BFncomms6624_CR38
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.183.3.1053
– volume: 301
  start-page: 1725
  year: 2003
  ident: BFncomms6624_CR14
  publication-title: Science
  doi: 10.1126/science.1085643
– volume: 271
  start-page: 25003
  year: 1996
  ident: BFncomms6624_CR19
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.271.40.25003
– volume: 210
  start-page: 1807
  year: 2013
  ident: BFncomms6624_CR27
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.20122528
– volume: 177
  start-page: 3930
  year: 2006
  ident: BFncomms6624_CR32
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.177.6.3930
– volume: 75
  start-page: 1553
  year: 1998
  ident: BFncomms6624_CR35
  publication-title: Biophys. J.
  doi: 10.1016/S0006-3495(98)74074-3
– volume: 34
  start-page: 1
  year: 2011
  ident: BFncomms6624_CR17
  publication-title: Immunity
  doi: 10.1016/j.immuni.2011.01.001
– volume: 189
  start-page: 253
  year: 1999
  ident: BFncomms6624_CR49
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.189.2.253
– volume: 179
  start-page: 7653
  year: 2007
  ident: BFncomms6624_CR34
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.179.11.7653
– volume: 23
  start-page: 7918
  year: 2004
  ident: BFncomms6624_CR12
  publication-title: Oncogene
  doi: 10.1038/sj.onc.1208081
– volume: 32
  start-page: 766
  year: 2010
  ident: BFncomms6624_CR15
  publication-title: Immunity
  doi: 10.1016/j.immuni.2010.05.011
– volume: 16
  start-page: 114
  year: 2004
  ident: BFncomms6624_CR22
  publication-title: Curr. Opin. Immunol.
  doi: 10.1016/j.coi.2003.11.008
– volume: 285
  start-page: 221
  year: 1999
  ident: BFncomms6624_CR26
  publication-title: Science
  doi: 10.1126/science.285.5425.221
– volume: 28
  start-page: 79
  year: 2010
  ident: BFncomms6624_CR7
  publication-title: Annu. Rev. Immunol.
  doi: 10.1146/annurev-immunol-030409-101308
– volume: 6
  start-page: 785
  year: 2005
  ident: BFncomms6624_CR23
  publication-title: Nat. Immunol.
  doi: 10.1038/ni1220
– volume: 75
  start-page: 761
  year: 2009
  ident: BFncomms6624_CR51
  publication-title: Cytometry A
  doi: 10.1002/cyto.a.20747
– volume: 228
  start-page: 9
  year: 2009
  ident: BFncomms6624_CR2
  publication-title: Immunol. Rev.
  doi: 10.1111/j.1600-065X.2008.00745.x
– volume: 23
  start-page: 7990
  year: 2004
  ident: BFncomms6624_CR40
  publication-title: Oncogene
  doi: 10.1038/sj.onc.1208074
– volume: 26
  start-page: 2093
  year: 1996
  ident: BFncomms6624_CR48
  publication-title: Eur. J. Immunol.
  doi: 10.1002/eji.1830260920
– volume: 16
  start-page: 418
  year: 2004
  ident: BFncomms6624_CR50
  publication-title: Curr. Opin. Immunol.
  doi: 10.1016/j.coi.2004.05.019
SSID ssj0000391844
Score 2.3935235
Snippet The earliest molecular events in T-cell recognition have not yet been fully described, and the initial T-cell receptor (TCR)-triggering mechanism remains a...
The earliest molecular events in T cell recognition have not yet been fully described, and the initial T cell receptor (TCR) triggering mechanism remains a...
SourceID pubmedcentral
proquest
pubmed
crossref
springer
SourceType Open Access Repository
Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 5624
SubjectTerms 13/109
13/95
14/33
14/35
14/63
631/250/1619/554/1775
631/250/21/324
82/29
82/83
96/31
96/33
96/35
Animals
CD3 Complex - genetics
CD3 Complex - metabolism
CD8 Antigens - genetics
CD8 Antigens - metabolism
Energy transfer
Female
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Humanities and Social Sciences
Ligands
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - genetics
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - metabolism
Major Histocompatibility Complex
Male
Mice
Mice, Knockout
multidisciplinary
Protein Binding
Receptors, Antigen, T-Cell - genetics
Receptors, Antigen, T-Cell - metabolism
Science
Science (multidisciplinary)
Synapses - enzymology
Synapses - genetics
Synapses - metabolism
T-Lymphocytes - metabolism
SummonAdditionalLinks – databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB5BERKXivIMlMqIXjhExY6TOCeEllYFFQ6olfYW-THergCn7aaH_ns8eZVlK67xyHFmHPubGfsbgH3HXSlQVKmVElNZyCI1yprUZ9Heyn_gGine8e17cXwmv87z-RBwWw3HKsc1sVuoXWMpRn7Ai-gc0DVO_vHiMqWqUZRdHUpo3IcHRF1Gzlc5L6cYC7GfKylHVtJMHYTY5e9VUQi5vg9tgMvNM5L_JEq7_efoMWwPwJF96i29A_cwPIGHfSnJm6fw5WS50MGlGBZxhXDsdPaDLVesjV-woHKczNywE_uThaZlejBJfEphWDb7rBixWdIJl-bqGZwdHZ7OjtOhTALpV7QpWl8aI02OjhcoueO5tlp6H8EJZpZztNpzzbV2toporqTMImqhszyLPZjsOWyFJuBLYK6S2mCuPLqoa6x09K8wgkAvhKea6Qm8H5VW24FDnEpZ_Kq7XHam6lsFJ_Bukr3omTPulNoddV8Pf8-qvrV1Am-n5jjvKZmhAzbXvYxSPALWBF70pppeE51eYrUXCZRrRpwEiFN7vSUszztubSmkElmVwP5o7r-GtTH6V_8f_Wt4FPGVpKuLotyFrfbqGt9EDNOavW6i_gEBZfTd
  priority: 102
  providerName: ProQuest
– databaseName: Springer Nature OA Free Journals
  dbid: C6C
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lb9QwEB5BERIX1PIMtMiIXjhE4EcS51gFqoIKB9RKvUV-jJdVwYu66aH_Hk9edFkOHBNPEmvGsb_xjL8BOPTcVwJFnTulMFelKnOrnc2DTPbW4T03SPsdX76WJ-fq80VxMdLkrMe0yoHSsp-mp-ywdzFd_VyXpVB34R5RtlP6XlM2834KMZ1rpSYGUqlvPbK55mwBye18yL-Cov1ac7wLD0eQyI6Gbu3BHYyP4P5QNvLmMXw6XS5M9DnGRZoNPDtrvrHlmnXJ0V5Q6U1mb9ipu2Rx1TEzqj_dpS1X1nzQjJgrKZtldfUEzo8_njUn-VgSgXQpuhxdqKxVtkDPS1Tc88I4o0JIQASl4xydCdxwY7yrE3KrKIqIRhhZyPQGK5_CTlxFfA7M18pYLHRAL5TE2iRfChPgC0IEqo-ewdtJaa0b-cKpbMWPto9bS93-UXAGb2bZXwNLxj-l9ifdt-Ofsm55mZw-Op7LM3g9N6cxToELE3F1PchozRM4zeDZYKr5M8nBJQZ7kUG1YcRZgPizN1vi8nvPo62E0kLWGRxO5r7Vra3ev_g_sZfwIGEqRccVRbUPO93VNR4k3NLZV_2A_Q2kN_El
  priority: 102
  providerName: Springer Nature
Title Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor
URI https://link.springer.com/article/10.1038/ncomms6624
https://www.ncbi.nlm.nih.gov/pubmed/25427562
https://www.proquest.com/docview/1628243941
https://www.proquest.com/docview/1628881161
https://pubmed.ncbi.nlm.nih.gov/PMC4248239
Volume 5
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwdV1bb9MwFD7aRUi8IMZtgVEZsRceAviSxHmYUBdWRrVNaKxS3yI7dkrFcKHNJPrvd5xLoXS8JJLtxNb5fPmOj30OwKGhJmGWpWEhhA1FLOJQy0KHJUe8ZfmeKuv3O84v4tORGI6j8RZ08TtbAS7uVO18PKnR_Prt71_LDzjgj5or4_KdQ2x-LOKYiW3YxRUp8SEczluaX8_IPEVFRnTeSdc-8d6AI-GdoLP1pWmDb24em_zHdlovSYOH8KDlkqTfgL8HW9Y9gntNdMnlY_h8Np0oZ0LrJjhpGHKVXZLpglSoj098hE6il-Ss-E7crCKqRQlT_c4syT5K4h1c-kMvs_kTGA1OrrLTsI2c4EXOqtAWZaK10JE1NLaCGhqpQomyRL5ieUGpLVRJFVXKFCkSvMQbG61iikcc_6D5U9hxM2f3gZhUKG0jWVrDBLepQpXLIi8sGSt9GPUA3nRCy4vWrbiPbnGd1-ZtLvM_sg7g9arsz8aZxp2lDjrZ511_yGmMuqG_xUsDeLXKxqHg7RvK2dlNU0ZKihw2gGcNVKtqOowDSNZAXBXwbrbXc9z0W-1uWzAhGU8DOOzg_qtZG61__t-qX8B9ZFvCX2RkyQHsVPMb-xIZTaV7sJ2ME3zKwace7Pb7w69DfB-fXHy5xNQsznp1h74FcND9OQ
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB6VIgQXxJtAASPKgUNU_EjiHBBCW5Zduu0BbaXegmM7ywrIlm4qtH-K38hMXmVZxK3XxHImM2N7Xv4GYNdxlwgv0tAq5UMVqzjMtc3DQqK8dfGaG0_xjsOjeHSsPp5EJ1vwq7sLQ2WV3Z5Yb9RuYSlGvsdjdA7oGid_e_ojpK5RlF3tWmg0anHgVz_RZVu-Ge-jfF8KMXw_HYzCtqsAkSOq0NsiyXOVR97x2CvueGSsUUWBZ7mXlnNvTcENN8bZFI2fhBJx3ggjI4kz5BLnvQJXlZQplRDq4Yc-pkNo61qpDgVV6r0Sf-H7Mo6FWj_3NozZzZrMvxKz9Xk3vAU3W0OVvWs06zZs-fIOXGtaV67uwngyn5nShb6c4Y7k2HTwic2XrEKOzaj9J8tXbGK_snJRMdOqAD6lsC8b7GtG6JlUUbM4uwfHl8LA-7BdLkr_EJhLlcl9pAvvULY-NejPeTQ6CyEK6tEewKuOaZltMcupdca3rM6dS51dMDiAF_3Y0wap45-jdjreZ-1qXWYXuhXA8_41rjNKnpjSL86bMVpzNJADeNCIqv8MOtmEoi8CSNaE2A8gDO_1N-X8S43lrYTSQqYB7Hbi_oOsDeof_Z_6Z3B9ND2cZJPx0cFjuIG2naJrkyLZge3q7Nw_Qfupyp_WSsvg82Wvkt_sUjPW
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtR3LbtQwcFSKQFwQbwIFjCgHDtHWjpM4B4TQLkuXLhVCrdRb6jj2smpJSjcV2l_j65jJqyyLuPWajJzJPOx5eQZgO-d5LKxIfCOl9WUkIz9TJvNdgPxWbodrS_GOz_vR7qH8dBQebcCv7i4MlVV2e2K9UeeloRj5gEfoHNA1Tj5wbVnEl9H43dkPnyZIUaa1G6fRiMieXf5E923xdjJCXr8WYvzhYLjrtxMGCDVR-da4OMtkFtqcR1bynIfaaOkcnus2MJxbox3XXOvcJGgIxZSUs1roIAxwhSzAda_B9ThQOzQ9QY0_9vEd6ryupOw6ogZqUODvfF9EkZCrZ-CaYbten_lXkrY--8Z34HZrtLL3jZTdhQ1b3IMbzRjL5X2YTOczXeS-LWa4O-XsYPiVzResQorNaBQoy5Zsak5YUVZMt-KATykEzIYjxaiTJlXXlOcP4PBKCPgQNouysI-B5YnUmQ2Vszny2SYafTuLBqgTwtG8dg_edERLTdu_nMZonKZ1Hj1Q6SWBPXjVw541XTv-CbXV0T5tNXeRXsqZBy_716hzlEjRhS0vGhilOBrLHjxqWNV_Bh1u6qgvPIhXmNgDUD_v1TfF_Fvd11sKqUSQeLDdsfsPtNawf_J_7F_ATdSPdDrZ33sKt9DMk3SDUsRbsFmdX9hnaEpV2fNaZhkcX7WS_AawozgD
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ligand-engaged+TCR+is+triggered+by+Lck+not+associated+with+CD8+coreceptor&rft.jtitle=Nature+communications&rft.au=Casas%2C+Javier&rft.au=Brzostek%2C+Joanna&rft.au=Zarnitsyna%2C+Veronika+I&rft.au=Hong%2C+Jin-sung&rft.date=2014-11-27&rft.eissn=2041-1723&rft.volume=5&rft.spage=5624&rft_id=info:doi/10.1038%2Fncomms6624&rft_id=info%3Apmid%2F25427562&rft.externalDocID=25427562
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2041-1723&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2041-1723&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2041-1723&client=summon