Reactive oxygen species and glutathione antioxidants in the testis of the soil biosentinel Podarcis sicula (Rafinesque 1810)

• Published in: Environmental science and pollution research international Vol. 25; no. 19; pp. 18286 - 18296
• Main Authors: Guerriero, Giulia, D’Errico, Gerardino, Di Giaimo, Rossella, Rabbito, Dea, Olanrewaju, Oladokun Sulaiman, Ciarcia, Gaetano
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2018; Springer Nature B.V
• Subjects: Adenosine diphosphate; Animals; antioxidant activity; Antioxidants; Antioxidants - metabolism; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Damage detection; Deoxyribonucleic acid; DNA; DNA damage; DNA repair; Dumping; Earth and Environmental Science; Ecotoxicology; Electron paramagnetic resonance; electron paramagnetic resonance spectroscopy; Electron spin; Electrophoretic mobility; Endocrine disruptors; Endocrine Disruptors - toxicity; endocrine-disrupting chemicals; Environment; Environmental Chemistry; Environmental Health; Environmental Monitoring - methods; Environmental Restoration and Remediation; Environmental science; Environmental Sustainability and Pollution Prevention; Enzymatic activity; enzyme activity; gene expression regulation; Glutathione; Glutathione - metabolism; Glutathione peroxidase; Glutathione transferase; Health care; Italy; Lipid peroxidation; lizards; Lizards - metabolism; Male; messenger RNA; NAD ADP-ribosyltransferase; Organic chemistry; Oxidative stress; Oxidative Stress - drug effects; Oxygen; Peroxidase; Peroxidation; phospholipid-hydroperoxide glutathione peroxidase; Podarcis; Podarcis siculus; pollution; Pollution index; Poly(ADP-ribose); Poly(ADP-ribose) polymerase; quantitative polymerase chain reaction; Reactive oxygen species; Reactive Oxygen Species - metabolism; Remediation; Repair; Reproductive health; Reproductive system; Reptiles; Ribose; screening; soil; Soil - chemistry; Soil investigations; Soil Pollutants - toxicity; Spectroscopy; Spin resonance; testes; Testis - drug effects; Testis - metabolism; Transcription; Waste Water Technology; Water Management; Water Pollution Control; Italy; Poly(ADP-ribosylat)ion; Total soluble antioxidant capacity; Real-time quantitative PCR, RTqPCR; Reactive oxygen species, ROS; Glutathione S-transferase, GST; Soil remediation assessment; Glutathione peroxidase 4, GPx4; Electron spin resonance, ESR

Important toxicological achievements have been made during the last decades using reptiles. We focus our investigation on gonadal reproductive health of the soil biosentinel Podarcis sicula which is very sensitive to endocrine-disrupting chemicals. The aim of this study is to quantitatively detect,...