Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities

• Published in: Systematic and applied microbiology Vol. 29; no. 3; pp. 244 - 252
• Main Authors: Toledo, F.L., Calvo, C., Rodelas, B., González-López, J.
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.04.2006; Elsevier
• Subjects: Alcaligenes; Alcaligenes faecalis; Bacillus; Bacillus (bacteria); Bacillus - classification; Bacillus - isolation & purification; Bacillus pumilus; Bacillus subtilis; Bacteriology; Biodegradation of pollutants; Biodegradation, Environmental; Bioemulsifier; Biological and medical sciences; bioremediation; Biotechnology; Enterobacter; Environment and pollution; fluoranthene; Fundamental and applied biological sciences. Psychology; Hydrocarbon; Industrial applications and implications. Economical aspects; Microbiology; Micrococcus; Micrococcus luteus; Miscellaneous; molecular sequence data; molecular systematics; naphthalene; Naphthalenes - metabolism; nucleotide sequences; PAH; petroleum; Petroleum - metabolism; Petroleum - microbiology; phenanthrene; Phenanthrenes - metabolism; phylogeny; pollutants; polluted soils; polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - metabolism; pyrene (hydrocarbon); Pyrenes - metabolism; ribosomal DNA; RNA, Ribosomal, 16S - chemistry; sequence analysis; soil bacteria; Soil Microbiology; Soil Pollutants; soil pollution; Enterobacter; Hydrocarbon; Alcaligenes; PAH; Bacillus; Bioemulsifier; Micrococcus; Identification; Bacillaceae; Polycyclic aromatic compound; Screening; Bacillales; Micrococcales; Bacteria; Micrococcaceae; Bioremediation; Enterobacteriaceae
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2005.09.003

Fifteen bacterial strains isolated from solid waste oil samples were selected due to their capacity of growing in the presence of hydrocarbons. The isolates were identified by PCR of the 16S rDNA gene using fD1 and rD1 primers. The majority of the strains belonged to genera Bacillus, Bacillus pumilus (eight strains) and Bacillus subtilis (two strains). Besides, three strains were identified as Micrococcus luteus, one as Alcaligenes faecalis and one strain as Enterobacter sp. Growth of the above-mentioned strains in mineral liquid media amended with naphthalene, phenanthrene, fluoranthene or pyrene as sole carbon source was studied and our results showed that these strains can tolerate and remove different polycyclic aromatic hydrocarbons that may be toxic in the environment polluted with hydrocarbons. Finally, the capacity of certain strains to emulsify octane, xilene, toluene, mineral oil and crude oil, and its ability to remove hydrocarbons, look promising for its application in bioremediation technologies.