Nebivolol Prevents Up-Regulation of Nox2/NADPH Oxidase and Lipoperoxidation in the Early Stages of Ethanol-Induced Cardiac Toxicity

• Published in: Cardiovascular toxicology Vol. 21; no. 3; pp. 224 - 235
• Main Authors: do Vale, Gabriel T., da Silva, Carla B. P., Sousa, Arthur H., Gonzaga, Natália A., Parente, Juliana M., Araújo, Katiúscia M., Castro, Michele M., Tirapelli, Carlos R.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2021; Springer; Springer Nature B.V
• Subjects: Acetaldehyde; Alcohol; Alcohol, Denatured; Animals; Biomedical and Life Sciences; Biomedicine; Cardiology; Cardiomyopathy, Alcoholic - enzymology; Cardiomyopathy, Alcoholic - etiology; Cardiomyopathy, Alcoholic - pathology; Cardiomyopathy, Alcoholic - prevention & control; Catalase; Catalase - metabolism; CYBB protein; Disease Models, Animal; Ethanol; Gelatinase A; Gelatinase B; Glutathione; Heart; Heart Ventricles - drug effects; Heart Ventricles - enzymology; Heart Ventricles - pathology; Lipid Peroxidation - drug effects; Male; Matrix metalloproteinase; Matrix metalloproteinases; Metalloproteinase; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - enzymology; Myocytes, Cardiac - pathology; NAD(P)H oxidase; NADPH Oxidase 2 - metabolism; Nebivolol; Nebivolol - pharmacology; Nitric Oxide Synthase Type III - metabolism; NOX4 protein; Oxidases; Pharmacology/Toxicology; Rac1 protein; Rats; Rats, Wistar; Redox properties; Superoxide; Superoxide dismutase; Superoxides - metabolism; Thiobarbituric acid; Tissue inhibitor of metalloproteinase 2; Tissue Inhibitor of Metalloproteinase-2 - metabolism; Toxicity; Up-Regulation; Ventricle; Nebivolol; Reactive oxygen species; Matrix metalloproteinase; Ethanol; Left ventricle
• ISSN: 1530-7905; 1559-0259
• DOI: 10.1007/s12012-020-09614-1

Changes in redox state are described in the early stages of ethanol-induced cardiac toxicity. Here, we evaluated whether nebivolol would abrogate ethanol-induced redox imbalance in the heart. Male Wistar rats were treated with a solution of ethanol (20% v/v) for 3 weeks. Treatment with nebivolol (10 mg/kg/day; p.o. gavage) prevented the increase of both superoxide (O 2 •- ) and thiobarbituric acid reactive substances (TBARS) in the left ventricle of rats chronically treated with ethanol. Neither ethanol nor nebivolol affected the expression of Nox4, p47 phox , or Rac-1. Nebivolol prevented ethanol-induced increase of Nox2 expression in the left ventricle. Superoxide dismutase (SOD) activity as well as the concentration of reduced glutathione (GSH) was not altered by ethanol or nebivolol. Augmented catalase activity was detected in the left ventricle of both ethanol- and nebivolol-treated rats. Treatment with nebivolol, but not ethanol increased eNOS expression in the left ventricle. No changes in the activity of matrix metalloproteinase (MMP)2 or in the expressions of MMP2, MMP9, and tissue inhibitor metalloproteinase (TIMP)1 were detected after treatment with ethanol or nebivolol. However, ethanol increased the expression of TIMP2, and this response was prevented by nebivolol. Our results provided novel insights into the mechanisms underlying the early stages of the cardiac injury induced by ethanol consumption. We demonstrated that Nox2/NADPH oxidase-derived ROS play a role in ethanol-induced lipoperoxidation and that this response was prevented by nebivolol. In addition, we provided evidence that MMPs are not activated in the early stages of ethanol-induced cardiac toxicity.