Development of an artificial synovial fluid useful for studying Staphylococcus epidermidis joint infections
Staphylococcus epidermidis is a major causative agent of prosthetic joint infections (PJI). The ability to form biofilms supports this highly selective pathogenic potential. In vitro studies essentially relying on phenotypic assays and genetic approaches have provided a detailed picture of the molec...
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Published in | Frontiers in cellular and infection microbiology Vol. 12; p. 948151 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
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29.07.2022
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Abstract | Staphylococcus epidermidis
is a major causative agent of prosthetic joint infections (PJI). The ability to form biofilms supports this highly selective pathogenic potential.
In vitro
studies essentially relying on phenotypic assays and genetic approaches have provided a detailed picture of the molecular events contributing to biofilm assembly. A major limitation in these studies is the use of synthetic growth media, which significantly differs from the environmental conditions
S. epidermidis
encounters during host invasion. Building on evidence showing that growth in serum substantially affects
S. epidermidis
gene expression profiles and phenotypes, the major aim of this study was to develop and characterize a growth medium mimicking synovial fluid, thereby facilitating research addressing specific aspects related to PJI. Using fresh human plasma, a protocol was established allowing for the large-scale production of a medium that by biochemical analysis matches key characteristics of synovial fluid and therefore is referred to as artificial synovial fluid (ASF). By analysis of biofilm-positive, polysaccharide intercellular adhesion (PIA)-producing
S. epidermidis
1457 and its isogenic, PIA- and biofilm-negative mutant 1457-M10, evidence is provided that the presence of ASF induces cluster formation in
S. epidermidis
1457 and mutant 1457-M10. Consistent with the aggregative properties, both strains formed multilayered biofilms when analyzed by confocal laser scanning microscopy. In parallel to the phenotypic findings, expression analysis after growth in ASF found upregulation of genes encoding for intercellular adhesins (
icaA
,
aap
, and
embp
) as well as
atlE
, encoding for the major cell wall autolysin being responsible for eDNA release. In contrast, growth in ASF was associated with reduced expression of the master regulator
agr
. Collectively, these results indicate that ASF induces expression profiles that are able to support intercellular adhesion in both PIA-positive and PIA-negative
S. epidermidis
. Given the observation that ASF overall induced biofilm formation in a collection of
S. epidermidis
isolates from PJI, the results strongly support the idea of using growth media mimicking host environments. ASF may play an important role in future studies related to the pathogenesis of
S. epidermidis
PJI. |
---|---|
AbstractList | Staphylococcus epidermidis
is a major causative agent of prosthetic joint infections (PJI). The ability to form biofilms supports this highly selective pathogenic potential.
In vitro
studies essentially relying on phenotypic assays and genetic approaches have provided a detailed picture of the molecular events contributing to biofilm assembly. A major limitation in these studies is the use of synthetic growth media, which significantly differs from the environmental conditions
S. epidermidis
encounters during host invasion. Building on evidence showing that growth in serum substantially affects
S. epidermidis
gene expression profiles and phenotypes, the major aim of this study was to develop and characterize a growth medium mimicking synovial fluid, thereby facilitating research addressing specific aspects related to PJI. Using fresh human plasma, a protocol was established allowing for the large-scale production of a medium that by biochemical analysis matches key characteristics of synovial fluid and therefore is referred to as artificial synovial fluid (ASF). By analysis of biofilm-positive, polysaccharide intercellular adhesion (PIA)-producing
S. epidermidis
1457 and its isogenic, PIA- and biofilm-negative mutant 1457-M10, evidence is provided that the presence of ASF induces cluster formation in
S. epidermidis
1457 and mutant 1457-M10. Consistent with the aggregative properties, both strains formed multilayered biofilms when analyzed by confocal laser scanning microscopy. In parallel to the phenotypic findings, expression analysis after growth in ASF found upregulation of genes encoding for intercellular adhesins (
icaA
,
aap
, and
embp
) as well as
atlE
, encoding for the major cell wall autolysin being responsible for eDNA release. In contrast, growth in ASF was associated with reduced expression of the master regulator
agr
. Collectively, these results indicate that ASF induces expression profiles that are able to support intercellular adhesion in both PIA-positive and PIA-negative
S. epidermidis
. Given the observation that ASF overall induced biofilm formation in a collection of
S. epidermidis
isolates from PJI, the results strongly support the idea of using growth media mimicking host environments. ASF may play an important role in future studies related to the pathogenesis of
S. epidermidis
PJI. Staphylococcus epidermidis is a major causative agent of prosthetic joint infections (PJI). The ability to form biofilms supports this highly selective pathogenic potential. In vitro studies essentially relying on phenotypic assays and genetic approaches have provided a detailed picture of the molecular events contributing to biofilm assembly. A major limitation in these studies is the use of synthetic growth media, which significantly differs from the environmental conditions S. epidermidis encounters during host invasion. Building on evidence showing that growth in serum substantially affects S. epidermidis gene expression profiles and phenotypes, the major aim of this study was to develop and characterize a growth medium mimicking synovial fluid, thereby facilitating research addressing specific aspects related to PJI. Using fresh human plasma, a protocol was established allowing for the large-scale production of a medium that by biochemical analysis matches key characteristics of synovial fluid and therefore is referred to as artificial synovial fluid (ASF). By analysis of biofilm-positive, polysaccharide intercellular adhesion (PIA)-producing S. epidermidis 1457 and its isogenic, PIA- and biofilm-negative mutant 1457-M10, evidence is provided that the presence of ASF induces cluster formation in S. epidermidis 1457 and mutant 1457-M10. Consistent with the aggregative properties, both strains formed multilayered biofilms when analyzed by confocal laser scanning microscopy. In parallel to the phenotypic findings, expression analysis after growth in ASF found upregulation of genes encoding for intercellular adhesins (icaA, aap, and embp) as well as atlE, encoding for the major cell wall autolysin being responsible for eDNA release. In contrast, growth in ASF was associated with reduced expression of the master regulator agr. Collectively, these results indicate that ASF induces expression profiles that are able to support intercellular adhesion in both PIA-positive and PIA-negative S. epidermidis. Given the observation that ASF overall induced biofilm formation in a collection of S. epidermidis isolates from PJI, the results strongly support the idea of using growth media mimicking host environments. ASF may play an important role in future studies related to the pathogenesis of S. epidermidis PJI. |
Author | Linder, Stefan Rohde, Holger Failla, Antonio Virgilio Weißelberg, Samira Both, Anna Aepfelbacher, Martin Nordholt, Gerhard Stamm, Johanna Büttner, Henning |
AuthorAffiliation | 1 Institut für Medizinische Mikrobiologie, Virologie und Hygiene , Hamburg , Germany 4 Deutsches Zentrum für Infektionsmedizin, Standort Hamburg-Lübeck-Borstel , Hamburg , Germany 3 Institute for Clinical Chemistry, Universitätsklinikum Hamburg-Eppendorf , Hamburg , Germany 2 Microscopy Imaging Facility, University Medical Center Hamburg-Eppendorf , Hamburg , Germany |
AuthorAffiliation_xml | – name: 4 Deutsches Zentrum für Infektionsmedizin, Standort Hamburg-Lübeck-Borstel , Hamburg , Germany – name: 1 Institut für Medizinische Mikrobiologie, Virologie und Hygiene , Hamburg , Germany – name: 3 Institute for Clinical Chemistry, Universitätsklinikum Hamburg-Eppendorf , Hamburg , Germany – name: 2 Microscopy Imaging Facility, University Medical Center Hamburg-Eppendorf , Hamburg , Germany |
Author_xml | – sequence: 1 givenname: Johanna surname: Stamm fullname: Stamm, Johanna – sequence: 2 givenname: Samira surname: Weißelberg fullname: Weißelberg, Samira – sequence: 3 givenname: Anna surname: Both fullname: Both, Anna – sequence: 4 givenname: Antonio Virgilio surname: Failla fullname: Failla, Antonio Virgilio – sequence: 5 givenname: Gerhard surname: Nordholt fullname: Nordholt, Gerhard – sequence: 6 givenname: Henning surname: Büttner fullname: Büttner, Henning – sequence: 7 givenname: Stefan surname: Linder fullname: Linder, Stefan – sequence: 8 givenname: Martin surname: Aepfelbacher fullname: Aepfelbacher, Martin – sequence: 9 givenname: Holger surname: Rohde fullname: Rohde, Holger |
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CitedBy_id | crossref_primary_10_3390_biomedicines12020449 crossref_primary_10_1128_spectrum_00565_24 crossref_primary_10_1128_cmr_00024_23 crossref_primary_10_1016_j_biomaterials_2024_122578 crossref_primary_10_3390_foods12030514 |
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ContentType | Journal Article |
Copyright | Copyright © 2022 Stamm, Weißelberg, Both, Failla, Nordholt, Büttner, Linder, Aepfelbacher and Rohde 2022 Stamm, Weißelberg, Both, Failla, Nordholt, Büttner, Linder, Aepfelbacher and Rohde |
Copyright_xml | – notice: Copyright © 2022 Stamm, Weißelberg, Both, Failla, Nordholt, Büttner, Linder, Aepfelbacher and Rohde 2022 Stamm, Weißelberg, Both, Failla, Nordholt, Büttner, Linder, Aepfelbacher and Rohde |
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Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ORCID: Samira Weißelberg, https://orcid.org/0000-0001-7997-4999; Henning Büttner, https://orcid.org/0000-0002-5086-4961; Stefan Linder, https://orcid.org/0000-0001-8226-2802; Holger Rohde, https://orcid.org/0000-0001-8587-4433 Reviewed by: Timothy J Foster, Trinity College Dublin, Ireland; Andrew B Herr, Cincinnati Children’s Hospital Medical Center, United States Edited by: Gowrishankar Muthukrishnan, University of Rochester, United States This article was submitted to Bacteria and Host, a section of the journal Frontiers in Cellular and Infection Microbiology These authors have contributed equally to this work and share first authorship |
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Snippet | Staphylococcus epidermidis
is a major causative agent of prosthetic joint infections (PJI). The ability to form biofilms supports this highly selective... Staphylococcus epidermidis is a major causative agent of prosthetic joint infections (PJI). The ability to form biofilms supports this highly selective... |
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SubjectTerms | biofilm formation Cellular and Infection Microbiology confocal laser scanning electron microscope joint infection prosthetic joint infection (PJI) Staphylococcus epidermidis (S. epidermidis) |
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Title | Development of an artificial synovial fluid useful for studying Staphylococcus epidermidis joint infections |
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