Analysis of cephalosporins by hydrophilic interaction chromatography

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 54; no. 3; pp. 623 - 628
• Main Authors: Liu, Qiaoxia, Xu, Lingyan, Ke, Yanxiong, Jin, Yu, Zhang, Feifang, Liang, Xinmiao
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.02.2011; Elsevier
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - chemistry; Biological and medical sciences; Buffers; cefotaxime; Cephalosporins; Cephalosporins - analysis; Cephalosporins - chemistry; Chromatography, Liquid - methods; Chromatography, Reverse-Phase; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; Hydrophilic interaction chromatography; Hydrophobic and Hydrophilic Interactions; Medical sciences; Pharmacology. Drug treatments; Reproducibility of Results; Reversed-phase high performance liquid chromatography; silica; Silicon Dioxide; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; The Click β-CD column; The Click β-CD column; Reversed-phase high performance liquid chromatography; Cephalosporins; Hydrophilic interaction chromatography; Cephalosporin derivatives; β-Lactams; Analysis; Hydrophilic Interaction Liquid chromatography; HPLC chromatography

A simple hydrophilic interaction chromatography (HILIC) method was developed to analyze seven cephalosporins. These seven cephalosporins could be separated well on the Click β-CD column and Atlantis HILIC Silica column. The effects of buffer concentration and pH on the retention under HILIC mode wer...