Rhodamine B as ligand for affinity chromatography. Fixation studies onto cellulose by a curing method

• Published in: European polymer journal Vol. 42; no. 10; pp. 2270 - 2282
• Main Authors: Silva, Andreia, Boto, Renato E.F., El-Shishtawy, Reda M., Almeida, Paulo
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2006; Elsevier
• Subjects: Applied sciences; Cellulose; Cellulose and derivatives; Chromatography; Curing of polymers; Dyes; Exact sciences and technology; Natural polymers; Physicochemistry of polymers; Rhodamine B; Dyes; Rhodamine B; Curing of polymers; Chromatography; Cellulose; Rhodamine; Catalytic reaction; Organic ligand; Acid catalysis; Experimental study; Esterification; Lateral group; Optimization; Operating conditions; Chemical modification; Affinity chromatography; Preparation; Reaction mechanism; Cellulose organic ester; Stationary phase; Heterogeneous reaction

Rhodamine B was post-grafted onto cellulose in order to be lately used for affinity chromatography as a biomimetic ligand. A curing method has been developed to bound rhodamine onto cellulose via esterification, by heating an intimate mixture of both, at different temperatures above 180 °C. The lact...