A Murine Viral Outgrowth Assay to Detect Residual HIV Type 1 in Patients With Undetectable Viral Loads

• Published in: The Journal of infectious diseases Vol. 212; no. 9; pp. 1387 - 1396
• Main Authors: Pate, Kelly A. Metcalf, Pohlmeyer, Christopher W., Walker-Sperling, Victoria E., Foote, Jeremy B., Najarro, Kevin M., Cryer, Catherine G., Salgado, Maria, Gama, Lucio, Engle, Elizabeth L., Shirk, Erin N., Queen, Suzanne E., Chioma, Stanley, Vermillion, Meghan S., Bullock, Brandon, Li, Ming, Lyons, Claire E., Adams, Robert J., Zink, M. Christine, Clements, Janice E., Mankowski, Joseph L., Blankson, Joel N.
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.11.2015
• Subjects: Animals; Antiretroviral Therapy, Highly Active; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - virology; Disease Models, Animal; HIV Infections - diagnosis; HIV Infections - drug therapy; HIV-1 - growth & development; HIV-1 - isolation & purification; HIV/AIDS; Humans; Interleukin-2 - blood; Leukocytes, Mononuclear - virology; Macaca; Major and Brief Reports; Male; Mice; Simian immunodeficiency virus; Simian Immunodeficiency Virus - growth & development; Simian Immunodeficiency Virus - isolation & purification; Swine influenza virus; Viral Load; Viremia - veterinary; SIV; humanized mouse; cure; HIV; quantitative viral outgrowth assay (QVOA)

Background. Sensitive assays are needed for detection of residual human immunodeficiency virus (HIV) in patients with undetectable plasma viral loads to determine whether eradication strategies are effective. The gold standard quantitative viral outgrowth assay (QVOA) underestimates the magnitude of...