Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel
Mechanical compression is a double-edged sword for cartilage remodeling, and the effect of mechanical compression on chondrogenic differentiation still remains elusive to date. Herein, we investigate the effect of mechanical dynamic compression on the chondrogenic differentiation of human synovium-d...
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| Published in | Frontiers in bioengineering and biotechnology Vol. 9; p. 697281 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Frontiers Media S.A
19.07.2021
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| Abstract | Mechanical compression is a double-edged sword for cartilage remodeling, and the effect of mechanical compression on chondrogenic differentiation still remains elusive to date. Herein, we investigate the effect of mechanical dynamic compression on the chondrogenic differentiation of human synovium-derived mesenchymal stem cells (SMSCs). To this aim, SMSCs encapsulated in agarose hydrogels were cultured in chondrogenic-induced medium with or without dynamic compression. Dynamic compression was applied at either early time-point (day 1) or late time-point (day 21) during chondrogenic induction period. We found that dynamic compression initiated at early time-point downregulated the expression level of chondrocyte-specific markers as well as hypertrophy-specific markers compared with unloaded control. On the contrary, dynamic compression applied at late time-point not only enhanced the levels of cartilage matrix gene expression, but also suppressed the hypertrophic development of SMSCs compared with unloaded controls. Taken together, our findings suggest that dynamic mechanical compression loading not only promotes chondrogenic differentiation of SMSCs, but also plays a vital role in the maintenance of cartilage phenotype, and our findings also provide an experimental guide for stem cell-based cartilage repair and regeneration. |
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| AbstractList | Mechanical compression is a double-edged sword for cartilage remodeling, and the effect of mechanical compression on chondrogenic differentiation still remains elusive to date. Herein, we investigate the effect of mechanical dynamic compression on the chondrogenic differentiation of human synovium-derived mesenchymal stem cells (SMSCs). To this aim, SMSCs encapsulated in agarose hydrogels were cultured in chondrogenic-induced medium with or without dynamic compression. Dynamic compression was applied at either early time-point (day 1) or late time-point (day 21) during chondrogenic induction period. We found that dynamic compression initiated at early time-point downregulated the expression level of chondrocyte-specific markers as well as hypertrophy-specific markers compared with unloaded control. On the contrary, dynamic compression applied at late time-point not only enhanced the levels of cartilage matrix gene expression, but also suppressed the hypertrophic development of SMSCs compared with unloaded controls. Taken together, our findings suggest that dynamic mechanical compression loading not only promotes chondrogenic differentiation of SMSCs, but also plays a vital role in the maintenance of cartilage phenotype, and our findings also provide an experimental guide for stem cell-based cartilage repair and regeneration. |
| Author | Li, Yixuan Wang, Zixu Li, Lan Jiang, Qing Teng, Huajian Ge, Yuxiang |
| AuthorAffiliation | 1 State Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School , Nanjing , China 3 Jiangsu Engineering Research Center for 3D Bioprinting , Nanjing , China 2 Laboratory for Bone and Joint Disease, Model Animal Research Center, Nanjing University , Nanjing , China |
| AuthorAffiliation_xml | – name: 2 Laboratory for Bone and Joint Disease, Model Animal Research Center, Nanjing University , Nanjing , China – name: 3 Jiangsu Engineering Research Center for 3D Bioprinting , Nanjing , China – name: 1 State Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School , Nanjing , China |
| Author_xml | – sequence: 1 givenname: Yuxiang surname: Ge fullname: Ge, Yuxiang – sequence: 2 givenname: Yixuan surname: Li fullname: Li, Yixuan – sequence: 3 givenname: Zixu surname: Wang fullname: Wang, Zixu – sequence: 4 givenname: Lan surname: Li fullname: Li, Lan – sequence: 5 givenname: Huajian surname: Teng fullname: Teng, Huajian – sequence: 6 givenname: Qing surname: Jiang fullname: Jiang, Qing |
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| Cites_doi | 10.1242/jcs.108.4.1497 10.1053/joca.2001.0504 10.1002/art.23143 10.1080/14653240701291620 10.22203/eCM.v019a08 10.1089/scd.2011.0376 10.1186/scrt211 10.1080/08977190500252763 10.1002/jor.23670 10.1021/acsnano.5b06663 10.1089/ten.TEA.2011.0455 10.1002/art.23370 10.1186/ar1827 10.2106/JBJS.I.00895 10.1186/s12863-021-00967-2 10.21037/atm.2019.06.77 10.1089/ten.TEA.2009.0262 10.1016/j.joca.2007.03.008 10.1002/art.22136 10.1089/ten.tea.2011.0198 10.1186/ar614 10.1302/0301-620X.87B4.15971 10.1002/bdrc.21058 10.1016/j.bbrc.2003.09.099 10.1111/j.1432-0436.2008.00299.x 10.1038/nprot.2009.63 10.1016/j.archger.2010.03.026 10.1002/art.21212 10.1089/ten.TEA.2016.0376 10.1089/ten.2006.12.469 10.1016/j.joca.2005.09.002 10.1016/j.bbrc.2008.09.154 10.1089/107632703768247377 10.1634/stemcells.2006-0435 10.1021/acsbiomaterials.6b00587 10.1016/j.biomaterials.2014.10.010 10.1039/C6TB00006A 10.1016/j.jbiomech.2007.06.002 10.1016/j.biomaterials.2012.08.019 10.1007/s10439-010-0059-6 10.1089/scd.2008.0219 10.1172/JCI34174 10.1634/stemcells.2008-0616 10.1002/bies.950171208 10.1089/ten.TEA.2010.0043 10.1006/excr.1995.1401 10.1002/term.2461 10.1089/ten.TEA.2008.0357 |
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| References | Smith (B36) 2005; 87 Mouw (B26) 2007; 25 Holmvall (B16) 1995; 221 Shi (B35) 2016; 10 Fahy (B10) 2018; 36 Pelttari (B32) 2006; 54 Lima (B23) 2007; 15 Haugh (B14) 2011; 17 Demarteau (B8) 2003; 310 Horie (B17) 2009; 27 Li (B21) 2012; 33 De Bari (B7) 2008; 58 Ge (B12) 2019; 7 Li (B22) 2010; 16 Zhang (B47) 2015; 38 Hellingman (B15) 2011; 17 McKee (B25) 2017; 23 Mueller (B27) 2008; 58 Huang (B18) 2010; 19 Chen (B6) 2021; 22 Jo (B19) 2007; 9 Yang (B45) 2009; 18 Bougault (B4) 2009; 4 Schiavi (B34) 2018; 12 Pei (B31) 2008; 76 García-Álvarez (B11) 2011; 52 Bedi (B2) 2010; 92 Yang (B46) 2012; 21 Djouad (B9) 2005; 7 Toh (B39) 2005; 23 Wang (B43) 2014; 102 O’Conor (B30) 2013; 4 Tuan (B40) 2003; 5 Kisiday (B20) 2009; 15 Thorpe (B38) 2010; 38 Muir (B28) 1995; 17 Verteramo (B41) 2007; 40 Thorpe (B37) 2008; 377 O’Connell (B29) 2017; 3 Zuscik (B48) 2008; 118 Wakitani (B42) 2002; 10 Aisenbrey (B1) 2016; 4 Gemmiti (B13) 2006; 12 Bian (B3) 2012; 18 Buschmann (B5) 1995; 108 Sakaguchi (B33) 2005; 52 Mauck (B24) 2006; 14 Williams (B44) 2003; 9 |
| References_xml | – volume: 108 start-page: 1497 year: 1995 ident: B5 article-title: Mechanical compression modulates matrix biosynthesis in chondrocyte/agarose culture. publication-title: J. Cell Sci. doi: 10.1242/jcs.108.4.1497 contributor: fullname: Buschmann – volume: 10 start-page: 199 year: 2002 ident: B42 article-title: Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair of cartilage defects in osteoarthritic knees. publication-title: Osteoarthr. Cartil. doi: 10.1053/joca.2001.0504 contributor: fullname: Wakitani – volume: 58 start-page: 240 year: 2008 ident: B7 article-title: A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells. publication-title: Arthritis Rheum. doi: 10.1002/art.23143 contributor: fullname: De Bari – volume: 9 start-page: 316 year: 2007 ident: B19 article-title: Surface characterization and chondrogenic differentiation of mesenchymal stromal cells derived from synovium. publication-title: Cytotherapy doi: 10.1080/14653240701291620 contributor: fullname: Jo – volume: 19 start-page: 72 year: 2010 ident: B18 article-title: Long-term dynamic loading improves the mechanical properties of chondrogenic mesenchymal stem cell-laden hydrogel. publication-title: Eur. Cells Mater. doi: 10.22203/eCM.v019a08 contributor: fullname: Huang – volume: 21 start-page: 1966 year: 2012 ident: B46 article-title: Temporal activation of beta-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated. publication-title: Stem Cells Dev. doi: 10.1089/scd.2011.0376 contributor: fullname: Yang – volume: 4 start-page: 61 year: 2013 ident: B30 article-title: Mechanical regulation of chondrogenesis. publication-title: Stem Cell. Res. Ther. doi: 10.1186/scrt211 contributor: fullname: O’Conor – volume: 23 start-page: 313 year: 2005 ident: B39 article-title: Combined effects of TGFbeta1 and BMP2 in serum-free chondrogenic differentiation of mesenchymal stem cells induced hyaline-like cartilage formation. publication-title: Growth Factors doi: 10.1080/08977190500252763 contributor: fullname: Toh – volume: 36 start-page: 52 year: 2018 ident: B10 article-title: Mechanical stimulation of mesenchymal stem cells: implications for cartilage tissue engineering. publication-title: J. Orthop. Res. doi: 10.1002/jor.23670 contributor: fullname: Fahy – volume: 10 start-page: 1292 year: 2016 ident: B35 article-title: Photo-cross-linked scaffold with kartogenin-encapsulated nanoparticles for cartilage regeneration. publication-title: ACS Nano doi: 10.1021/acsnano.5b06663 contributor: fullname: Shi – volume: 18 start-page: 715 year: 2012 ident: B3 article-title: Dynamic compressive loading enhances cartilage matrix synthesis and distribution and suppresses hypertrophy in hMSC-laden hyaluronic acid hydrogels. publication-title: Tissue Eng. Part A doi: 10.1089/ten.TEA.2011.0455 contributor: fullname: Bian – volume: 58 start-page: 1377 year: 2008 ident: B27 article-title: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells. publication-title: Arthritis Rheum. doi: 10.1002/art.23370 contributor: fullname: Mueller – volume: 7 start-page: R1304 year: 2005 ident: B9 article-title: Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells. publication-title: Arthritis Res. Ther. doi: 10.1186/ar1827 contributor: fullname: Djouad – volume: 92 start-page: 994 year: 2010 ident: B2 article-title: Management of articular cartilage defects of the knee. publication-title: J. Bone Joint Surg. Am. doi: 10.2106/JBJS.I.00895 contributor: fullname: Bedi – volume: 22 start-page: 13 year: 2021 ident: B6 article-title: Long-term dynamic compression enhancement TGF-beta3-induced chondrogenesis in bovine stem cells: a gene expression analysis. publication-title: BMC Genom. Data doi: 10.1186/s12863-021-00967-2 contributor: fullname: Chen – volume: 7 start-page: 336 year: 2019 ident: B12 article-title: Estrogen prevents articular cartilage destruction in a mouse model of AMPK deficiency via ERK-mTOR pathway. publication-title: Ann. Transl. Med. doi: 10.21037/atm.2019.06.77 contributor: fullname: Ge – volume: 16 start-page: 575 year: 2010 ident: B22 article-title: Chondrogenesis of human bone marrow mesenchymal stem cells in fibrin-polyurethane composites is modulated by frequency and amplitude of dynamic compression and shear stress. publication-title: Tissue Eng. Part A doi: 10.1089/ten.TEA.2009.0262 contributor: fullname: Li – volume: 15 start-page: 1025 year: 2007 ident: B23 article-title: The beneficial effect of delayed compressive loading on tissue-engineered cartilage constructs cultured with TGF-beta3. publication-title: Osteoarthr. Cartil. doi: 10.1016/j.joca.2007.03.008 contributor: fullname: Lima – volume: 54 start-page: 3254 year: 2006 ident: B32 article-title: Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice. publication-title: Arthritis Rheum. doi: 10.1002/art.22136 contributor: fullname: Pelttari – volume: 17 start-page: 3085 year: 2011 ident: B14 article-title: Temporal and spatial changes in cartilage-matrix-specific gene expression in mesenchymal stem cells in response to dynamic compression. publication-title: Tissue Eng. Part A doi: 10.1089/ten.tea.2011.0198 contributor: fullname: Haugh – volume: 5 start-page: 32 year: 2003 ident: B40 article-title: Adult mesenchymal stem cells and cell-based tissue engineering. publication-title: Arthritis Res. Ther. doi: 10.1186/ar614 contributor: fullname: Tuan – volume: 87 start-page: 445 year: 2005 ident: B36 article-title: A clinical review of cartilage repair techniques. publication-title: J. Bone Joint Surg. Br. doi: 10.1302/0301-620X.87B4.15971 contributor: fullname: Smith – volume: 102 start-page: 37 year: 2014 ident: B43 article-title: TGFbeta signaling in cartilage development and maintenance. publication-title: Birth Defects Res. C Embryo Today doi: 10.1002/bdrc.21058 contributor: fullname: Wang – volume: 310 start-page: 580 year: 2003 ident: B8 article-title: Dynamic compression of cartilage constructs engineered from expanded human articular chondrocytes. publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2003.09.099 contributor: fullname: Demarteau – volume: 76 start-page: 1044 year: 2008 ident: B31 article-title: Synovium-derived stem cell-based chondrogenesis. publication-title: Differentiation doi: 10.1111/j.1432-0436.2008.00299.x contributor: fullname: Pei – volume: 4 start-page: 928 year: 2009 ident: B4 article-title: Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures. publication-title: Nat. Protoc. doi: 10.1038/nprot.2009.63 contributor: fullname: Bougault – volume: 52 start-page: 239 year: 2011 ident: B11 article-title: Chondrogenic differentiation in femoral bone marrow-derived mesenchymal cells (MSC) from elderly patients suffering osteoarthritis or femoral fracture. publication-title: Arch. Gerontol. Geriatr. doi: 10.1016/j.archger.2010.03.026 contributor: fullname: García-Álvarez – volume: 52 start-page: 2521 year: 2005 ident: B33 article-title: Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. publication-title: Arthritis Rheum. doi: 10.1002/art.21212 contributor: fullname: Sakaguchi – volume: 23 start-page: 426 year: 2017 ident: B25 article-title: Compression induced chondrogenic differentiation of embryonic stem cells in three-dimensional polydimethylsiloxane scaffolds. publication-title: Tissue Eng. Part A doi: 10.1089/ten.TEA.2016.0376 contributor: fullname: McKee – volume: 12 start-page: 469 year: 2006 ident: B13 article-title: Fluid flow increases type II collagen deposition and tensile mechanical properties in bioreactor-grown tissue-engineered cartilage. publication-title: Tissue Eng. doi: 10.1089/ten.2006.12.469 contributor: fullname: Gemmiti – volume: 14 start-page: 179 year: 2006 ident: B24 article-title: Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture. publication-title: Osteoarthr. Cartil. doi: 10.1016/j.joca.2005.09.002 contributor: fullname: Mauck – volume: 377 start-page: 458 year: 2008 ident: B37 article-title: Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells. publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2008.09.154 contributor: fullname: Thorpe – volume: 9 start-page: 679 year: 2003 ident: B44 article-title: In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel. publication-title: Tissue Eng. doi: 10.1089/107632703768247377 contributor: fullname: Williams – volume: 25 start-page: 655 year: 2007 ident: B26 article-title: Dynamic compression regulates the expression and synthesis of chondrocyte-specific matrix molecules in bone marrow stromal cells. publication-title: Stem Cells doi: 10.1634/stemcells.2006-0435 contributor: fullname: Mouw – volume: 3 start-page: 2657 year: 2017 ident: B29 article-title: 3D Bioprinting: new directions in articular cartilage tissue engineering. publication-title: ACS Biomater. Sci. Eng. doi: 10.1021/acsbiomaterials.6b00587 contributor: fullname: O’Connell – volume: 38 start-page: 72 year: 2015 ident: B47 article-title: Cross-talk between TGF-beta/SMAD and integrin signaling pathways in regulating hypertrophy of mesenchymal stem cell chondrogenesis under deferral dynamic compression. publication-title: Biomaterials doi: 10.1016/j.biomaterials.2014.10.010 contributor: fullname: Zhang – volume: 4 start-page: 3562 year: 2016 ident: B1 article-title: Mechanical loading inhibits hypertrophy in chondrogenically differentiating hMSCs within a biomimetic hydrogel. publication-title: J. Mater. Chem. B doi: 10.1039/C6TB00006A contributor: fullname: Aisenbrey – volume: 40 start-page: 3580 year: 2007 ident: B41 article-title: Effect of a single impact loading on the structure and mechanical properties of articular cartilage. publication-title: J. Biomech. doi: 10.1016/j.jbiomech.2007.06.002 contributor: fullname: Verteramo – volume: 33 start-page: 8395 year: 2012 ident: B21 article-title: The influence of delayed compressive stress on TGF-beta1-induced chondrogenic differentiation of rat BMSCs through smad-dependent and smad-independent pathways. publication-title: Biomaterials doi: 10.1016/j.biomaterials.2012.08.019 contributor: fullname: Li – volume: 38 start-page: 2896 year: 2010 ident: B38 article-title: The response of bone marrow-derived mesenchymal stem cells to dynamic compression following TGF-beta3 induced chondrogenic differentiation. publication-title: Ann. Biomed. Eng. doi: 10.1007/s10439-010-0059-6 contributor: fullname: Thorpe – volume: 18 start-page: 929 year: 2009 ident: B45 article-title: Stage-dependent effect of TGF-beta1 on chondrogenic differentiation of human embryonic stem cells. publication-title: Stem Cells Dev. doi: 10.1089/scd.2008.0219 contributor: fullname: Yang – volume: 118 start-page: 429 year: 2008 ident: B48 article-title: Regulation of chondrogenesis and chondrocyte differentiation by stress. publication-title: J. Clin. Invest. doi: 10.1172/JCI34174 contributor: fullname: Zuscik – volume: 27 start-page: 878 year: 2009 ident: B17 article-title: Intra-articular injected synovial stem cells differentiate into meniscal cells directly and promote meniscal regeneration without mobilization to distant organs in rat massive meniscal defect. publication-title: Stem Cells doi: 10.1634/stemcells.2008-0616 contributor: fullname: Horie – volume: 17 start-page: 1039 year: 1995 ident: B28 article-title: The chondrocyte, architect of cartilage. biomechanics, structure, function and molecular biology of cartilage matrix macromolecules. publication-title: Bioessays doi: 10.1002/bies.950171208 contributor: fullname: Muir – volume: 17 start-page: 1157 year: 2011 ident: B15 article-title: Smad signaling determines chondrogenic differentiation of bone-marrow-derived mesenchymal stem cells: inhibition of Smad1/5/8P prevents terminal differentiation and calcification. publication-title: Tissue Eng. Part A doi: 10.1089/ten.TEA.2010.0043 contributor: fullname: Hellingman – volume: 221 start-page: 496 year: 1995 ident: B16 article-title: Chondrocyte and chondrosarcoma cell integrins with affinity for collagen type II and their response to mechanical stress. publication-title: Exp. Cell Res. doi: 10.1006/excr.1995.1401 contributor: fullname: Holmvall – volume: 12 start-page: 360 year: 2018 ident: B34 article-title: Mechanical stimulations on human bone marrow mesenchymal stem cells enhance cells differentiation in a three-dimensional layered scaffold. publication-title: J. Tissue Eng. Regen. Med. doi: 10.1002/term.2461 contributor: fullname: Schiavi – volume: 15 start-page: 2817 year: 2009 ident: B20 article-title: Dynamic compression stimulates proteoglycan synthesis by mesenchymal stem cells in the absence of chondrogenic cytokines. publication-title: Tissue Eng. Part A doi: 10.1089/ten.TEA.2008.0357 contributor: fullname: Kisiday |
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| StartPage | 697281 |
| SubjectTerms | Bioengineering and Biotechnology cartilage repair chondrocytes chondrogenesis mechanical compression SMSCs |
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| Title | Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel |
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