Protective effect of 5-hydroxymethylfurfural derived from processed Fructus Corni on human hepatocyte LO2 injured by hydrogen peroxide and its mechanism

• Published in: Journal of ethnopharmacology Vol. 128; no. 2; pp. 373 - 376
• Main Authors: Ding, Xia, Wang, Ming-Yan, Yao, Yun-Xiang, Li, Guan-Ye, Cai, Bao-Chang
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 24.03.2010; Amsterdam; New York: Elsevier
• Subjects: 5-Hydroxymethylfurfural; apoptosis; Apoptosis - drug effects; Biphenyl Compounds; Caspase 3 - metabolism; Caspase-3; Cell cycle; Cell Division - drug effects; Cell Line; Cell Survival - drug effects; Cornus - metabolism; Cornus officinalis; cultured cells; cysteine proteinases; enzyme activity; Fructus Corni; Fruit - metabolism; fruits (plant anatomy); Furaldehyde - analogs & derivatives; Furaldehyde - pharmacology; furfural; hepatocytes; Hepatocytes - metabolism; Hepatoprotective effect; herbal medicines; human cell lines; Humans; hydrogen peroxide; Hydrogen Peroxide - pharmacology; mechanism of action; medicinal plants; medicinal properties; Nitric oxide; Nitric Oxide - metabolism; plant extracts; Protective Agents - pharmacology; Tetrazolium Salts - metabolism; viability; PBS; FCM; 5-Hydroxymethylfurfural; Nitric oxide; Cell cycle; 5-HMF; PI; Fructus Corni; Hepatoprotective effect; MTT; Caspase-3
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2010.01.043

The aim of the present study was to evaluate the putative protective effect of 5-hydroxymethylfurfural (5-HMF) derived from processed Fructus Corni on human hepatocyte cell line (LO2) injured by hydrogen peroxide in vitro and the mechanism of its protection. The percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The hepatocyte cell apoptosis and cell cycle were detected by flow cytometric analysis. The content of nitric oxide and caspase-3 activity were quantified spectrophotometrically by enzyme-linked immunoassay. The study showed that incubation with 5-HMF caused significant increase in the viability of LO2 cell, decrease of cell apoptosis and recovery of cell cycle in LO2 cell injured by hydrogen peroxide, which was accompanied with the decreased nitric oxide level and caspase-3 activity. Our data suggest that 5-HMF protects LO2 cell against damage induced by hydrogen peroxide through inhibiting effect of cell apoptosis caused by promoting S phase to G2/M phase and the decreased caspase-3 activity and nitric oxide level. 5-HMF is one of the active principles in processed Fructus Corni.