No differential effects of divergent isocaloric supplements on signaling for muscle protein turnover during recovery from muscle-damaging eccentric exercise

• Published in: Amino acids Vol. 47; no. 4; pp. 767 - 778
• Main Authors: Rahbek, Stine Klejs, Farup, Jean, de Paoli, Frank, Vissing, Kristian
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.04.2015; Springer Nature B.V
• Subjects: Analytical Chemistry; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Dietary Supplements - analysis; Exercise; Humans; Life Sciences; Male; Muscle Proteins - metabolism; Muscle, Skeletal - injuries; Muscle, Skeletal - metabolism; Myalgia - etiology; Myalgia - metabolism; Neurobiology; Original Article; Proteomics; Resistance Training - adverse effects; Signal Transduction; Young Adult; Eccentric exercise; Whey protein; mTOR signaling; Recovery; MuRF1; FOXO1/3A signaling
• DOI: 10.1007/s00726-014-1907-8
• ISSN: 0939-4451

Unaccustomed high-intensity eccentric exercise (ECC) can provoke muscle damage including several days of muscle force loss. Post-exercise dietary supplementation may provide a strategy to accelerate rate of force regain by affecting mechanisms related to muscle protein turnover. The aim of the current study was to investigate if protein signaling mechanisms involved in muscle protein turnover would be differentially affected by supplementation with either whey protein hydrolysate and carbohydrate (WPH+CHO) versus isocaloric carbohydrate (CHO) after muscle-damaging ECC. Twenty-four young healthy participants received either WPH+CHO ( n  = 12) or CHO supplements ( n  = 12) during post-exercise recovery from 150 maximal unilateral eccentric contractions. Prior to, at 3 h and at 24, 48, 96 and/or 168 h post-exercise, muscle strength, muscle soreness, and Akt-mTOR and FOXO signaling proteins, were measured in an ECC exercising leg and in the contralateral non-exercise control leg (CON). After ECC, muscle force decreased by 23–27 % at 24 h post-exercise, which was followed by gradual, although not full recovery at 168 h post-exercise, with no differences between supplement groups. Phosphorylation of mTOR, p70S6K and rpS6 increased and phosphorylation of FOXO1 and FOXO3 decreased in the ECC leg, with no differences between supplement groups. Phosphorylation changes were also observed for rpS6, FOXO1 and FOXO3a in the CON leg, suggesting occurrence of remote tissue effects. In conclusion, divergent dietary supplementation types did not produce differences in signaling for muscle turnover during recovery from muscle-damaging exercise.