Consequences of the constitutive NOX2 activity in living cells: Cytosol acidification, apoptosis, and localized lipid peroxidation
The phagocyte NADPH oxidase (NOX2) is a key enzyme of the innate immune system generating superoxide anions (O2•-), precursors of reactive oxygen species. The NOX2 protein complex is composed of six subunits: two membrane proteins (gp91phox and p22phox) forming the catalytic core, three cytosolic pr...
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Published in | Biochimica et biophysica acta. Molecular cell research Vol. 1869; no. 9; p. 119276 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.09.2022
Elsevier |
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Abstract | The phagocyte NADPH oxidase (NOX2) is a key enzyme of the innate immune system generating superoxide anions (O2•-), precursors of reactive oxygen species. The NOX2 protein complex is composed of six subunits: two membrane proteins (gp91phox and p22phox) forming the catalytic core, three cytosolic proteins (p67phox, p47phox and p40phox) and a small GTPase Rac. The sophisticated activation mechanism of the NADPH oxidase relies on the assembly of cytosolic subunits with the membrane-bound components. A chimeric protein, called ‘Trimera’, composed of the essential domains of the cytosolic proteins p47phox (aa 1–286), p67phox (aa 1–212) and full-length Rac1Q61L, enables a constitutive and robust NOX2 activity in cells without the need of any stimulus. We employed Trimera as a single activating protein of the phagocyte NADPH oxidase in living cells and examined the consequences on the cell physiology of this continuous and long-term NOX activity. We showed that the sustained high level of NOX activity causes acidification of the intracellular pH, triggers apoptosis and leads to local peroxidation of lipids in the membrane. These local damages to the membrane correlate with the strong tendency of the Trimera to clusterize in the plasma membrane observed by FRET-FLIM microscopy.
•Trimera is a tool to trigger a continuous ROS production in living cells.•Continuous NOX2 activity causes cytosol acidification and apoptosis.•ROS overproduction leads to localized oxidation of the membrane lipids.•Trimera tends to clusterize in the plasma membrane of COSNOX and COS-7 cells. |
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AbstractList | The phagocyte NADPH oxidase (NOX2) is a key enzyme of the innate immune system generating superoxide anions (O
), precursors of reactive oxygen species. The NOX2 protein complex is composed of six subunits: two membrane proteins (gp91
and p22
) forming the catalytic core, three cytosolic proteins (p67
, p47
and p40
) and a small GTPase Rac. The sophisticated activation mechanism of the NADPH oxidase relies on the assembly of cytosolic subunits with the membrane-bound components. A chimeric protein, called 'Trimera', composed of the essential domains of the cytosolic proteins p47
(aa 1-286), p67
(aa 1-212) and full-length Rac1Q61L, enables a constitutive and robust NOX2 activity in cells without the need of any stimulus. We employed Trimera as a single activating protein of the phagocyte NADPH oxidase in living cells and examined the consequences on the cell physiology of this continuous and long-term NOX activity. We showed that the sustained high level of NOX activity causes acidification of the intracellular pH, triggers apoptosis and leads to local peroxidation of lipids in the membrane. These local damages to the membrane correlate with the strong tendency of the Trimera to clusterize in the plasma membrane observed by FRET-FLIM microscopy. The phagocyte NADPH oxidase (NOX2) is a key enzyme of the innate immune system generating superoxide anions (O2•-), precursors of reactive oxygen species. The NOX2 protein complex is composed of six subunits: two membrane proteins (gp91phox and p22phox) forming the catalytic core, three cytosolic proteins (p67phox, p47phox and p40phox) and a small GTPase Rac. The sophisticated activation mechanism of the NADPH oxidase relies on the assembly of cytosolic subunits with the membrane-bound components. A chimeric protein, called ‘Trimera’, composed of the essential domains of the cytosolic proteins p47phox (aa 1–286), p67phox (aa 1–212) and full-length Rac1Q61L, enables a constitutive and robust NOX2 activity in cells without the need of any stimulus. We employed Trimera as a single activating protein of the phagocyte NADPH oxidase in living cells and examined the consequences on the cell physiology of this continuous and long-term NOX activity. We showed that the sustained high level of NOX activity causes acidification of the intracellular pH, triggers apoptosis and leads to local peroxidation of lipids in the membrane. These local damages to the membrane correlate with the strong tendency of the Trimera to clusterize in the plasma membrane observed by FRET-FLIM microscopy. •Trimera is a tool to trigger a continuous ROS production in living cells.•Continuous NOX2 activity causes cytosol acidification and apoptosis.•ROS overproduction leads to localized oxidation of the membrane lipids.•Trimera tends to clusterize in the plasma membrane of COSNOX and COS-7 cells. |
ArticleNumber | 119276 |
Author | Nüsse, Oliver Erard, Marie Baciou, Laura Valenta, Hana Abdesselem, Mouna Bizouarn, Tania Dupré-Crochet, Sophie Deniset-Besseau, Ariane |
Author_xml | – sequence: 1 givenname: Hana surname: Valenta fullname: Valenta, Hana – sequence: 2 givenname: Sophie surname: Dupré-Crochet fullname: Dupré-Crochet, Sophie – sequence: 3 givenname: Mouna surname: Abdesselem fullname: Abdesselem, Mouna – sequence: 4 givenname: Tania surname: Bizouarn fullname: Bizouarn, Tania – sequence: 5 givenname: Laura surname: Baciou fullname: Baciou, Laura – sequence: 6 givenname: Oliver surname: Nüsse fullname: Nüsse, Oliver – sequence: 7 givenname: Ariane surname: Deniset-Besseau fullname: Deniset-Besseau, Ariane – sequence: 8 givenname: Marie surname: Erard fullname: Erard, Marie email: marie.erard@universite-paris-saclay.fr |
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Keywords | aa NADPH Oxidative stress PMA NMDG HRP AFM Lipid peroxidation IR FLIM FP FRET-FLIM ROI Intracellular pH NADPH oxidase ROS FRET DPI |
Language | English |
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Snippet | The phagocyte NADPH oxidase (NOX2) is a key enzyme of the innate immune system generating superoxide anions (O2•-), precursors of reactive oxygen species. The... The phagocyte NADPH oxidase (NOX2) is a key enzyme of the innate immune system generating superoxide anions (O ), precursors of reactive oxygen species. The... |
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SubjectTerms | Cellular Biology FRET-FLIM Intracellular pH Life Sciences Lipid peroxidation NADPH oxidase Oxidative stress ROS |
Title | Consequences of the constitutive NOX2 activity in living cells: Cytosol acidification, apoptosis, and localized lipid peroxidation |
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