Comparison of protective media and freezing techniques for cryopreservation of human semen

• Published in: European journal of obstetrics & gynecology and reproductive biology Vol. 91; no. 1; pp. 65 - 70
• Main Authors: Stanic, Patrik, Tandara, Marijan, Sonicki, Zdenko, Simunic, Velimir, Radakovic, Branko, Suchanek, Ernest
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.07.2000; Elsevier
• Subjects: Biological and medical sciences; Birth control; Buffers; Cryopreservation; Cryopreservation - methods; Culture Media; Gynecology. Andrology. Obstetrics; Human sperm; Humans; Male; Medical sciences; Semen - physiology; Sperm morphology; Sperm Motility; Spermatozoa - physiology; Sterility. Assisted procreation; Tromethamine - analogs & derivatives; Human sperm; Sperm motility; Cryopreservation; Sperm morphology; Human; Spermatozoa; Semen; Morphology; Mobility; Assisted procreation; Male; Technique; Solution

Objective: To evaluate the influence of cryopreservation medium and freezing–thawing techniques on human sperm motility and morphology. Study design: 63 semen samples were obtained from 39 donors to the artificial insemination programme. Possible effects of the sperm dilution with cryomedium on the...