Tristetraprolin Gene Single-Nucleotide Polymorphisms and mRNA Level in Patients With Rheumatoid Arthritis
To observe and evaluate the correlation between single-nucleotide polymorphisms (SNPs) and messenger RNA (mRNA) level related to tristetraprolin (TTP) in Chinese rheumatoid arthritis (RA). TapMan SNP was used for genotyping analysis in 580 RA patients and 554 healthy people. Association between TTP...
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Published in | Frontiers in pharmacology Vol. 12; p. 728015 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
01.09.2021
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Subjects | |
Online Access | Get full text |
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Summary: | To observe and evaluate the correlation between single-nucleotide polymorphisms (SNPs) and messenger RNA (mRNA) level related to tristetraprolin (TTP) in Chinese rheumatoid arthritis (RA). TapMan SNP was used for genotyping analysis in 580 RA patients and 554 healthy people. Association between
TTP
gene polymorphisms (rs251864 and rs3746083) and RA was obtained. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) technology was applied for the detection of TTP mRNA level in peripheral blood mononuclear cells (PBMCs) in 36 RA patients and 37 healthy people. We observed that the allele T of
TTP
rs3746083 increased RA susceptibility (
p
= 0.019). A significant difference was found under the dominant model of rs3746083 (
p
= 0.037). Further analysis showed the allele distribution of rs3746083 was nominally correlated with RF phenotype of RA patients (
p
= 0.045). Nevertheless, the association between
TTP
rs251864 and the incidence of RA was no statistically significant (
p
> 0.05). The TTP expression level in PBMCs of RA patients was significantly reduced (
p
< 0.001). In conclusion, the results of this experiment support that TTP may be involved in the pathogenesis of RA. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Hong Sheng Sun, Shandong Provincial Hospital, China Reviewed by:Tongyao Wang, Case Western Reserve University, United States These authors have contributed equally to this work and share first authorship Edited by:Tao Xu, Anhui Medical University, China This article was submitted to Inflammation Pharmacology, a section of the journal Frontiers in Pharmacology |
ISSN: | 1663-9812 1663-9812 |
DOI: | 10.3389/fphar.2021.728015 |