FnCas12a/crRNA-Mediated Genome Editing in Eimeria tenella

• Published in: Frontiers in genetics Vol. 12; p. 738746
• Main Authors: Cheng, Peipei, Zhang, Zhihao, Yang, Fayu, Cai, Shuo, Wang, Lina, Wang, Chunmei, Wang, Mi, Liu, Yingchun, Fei, Chenzhong, Zhang, Lifang, Xue, Feiqun, Gu, Feng
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 22.09.2021
• Subjects: Eimeria tenella; EtActin; EtHistone H4; FnCas12a; Genetics; genome editing; knock-in

Eimeria species are intracellular parasites residing inside the intestinal epithelial cell, which cause poultry coccidiosis and result in significant financial losses in the poultry industry. Genome editing of Eimeria is of immense importance for the development of vaccines and drugs. CRISPR/Cas9 ha...