UPLC-MS/MS Method for Analysis of Endocannabinoid and Related Lipid Metabolism in Mouse Mucosal Tissue

• Published in: Frontiers in physiology Vol. 12; p. 699712
• Main Authors: Wiley, Mark B., Perez, Pedro A., Argueta, Donovan A., Avalos, Bryant, Wood, Courtney P., DiPatrizio, Nicholas V.
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 14.07.2021
• Subjects: endocannabinoids; enzyme kinetics; fatty acid amide hydrolase; lipid metabolism; monoacylglycerol lipase; Physiology; UPLC-MS/MS

The endocannabinoid system is expressed in cells throughout the body and controls a variety of physiological and pathophysiological functions. We describe robust and reproducible UPLC-MS/MS-based methods for analyzing metabolism of the endocannabinoids, 2-arachidonoyl- sn -glycerol and arachidonoyl...