A portable microfluidic Aptamer-Tethered Enzyme Capture (APTEC) biosensor for malaria diagnosis

• Published in: Biosensors & bioelectronics Vol. 100; pp. 591 - 596
• Main Authors: Fraser, Lewis A., Kinghorn, Andrew B., Dirkzwager, Roderick M., Liang, Shaolin, Cheung, Yee-Wai, Lim, Bryce, Shiu, Simon Chi-Chin, Tang, Marco S.L., Andrew, Dean, Manitta, Joseph, Richards, Jack S., Tanner, Julian A.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.02.2018
• Subjects: 3D print; Aptamer; Aptamers, Nucleotide - chemistry; biomarkers; Biosensing Techniques - instrumentation; biosensors; colorimetry; Colorimetry - instrumentation; Diagnosis; DNA; Humans; L-Lactate Dehydrogenase - isolation & purification; lactate dehydrogenase; Limit of Detection; magnetism; Malaria; Malaria - blood; Malaria - diagnosis; microbeads; Microfluidic; Microfluidic Analytical Techniques - instrumentation; Models, Molecular; oligonucleotides; parasites; patients; Plasmodium falciparum; Plasmodium falciparum - enzymology; Plasmodium falciparum - isolation & purification; point-of-care systems; Printing, Three-Dimensional; prototypes; 3D print; Plasmodium falciparum; Aptamer; Diagnosis; Malaria; Microfluidic

There is a critical need for better biosensors for the detection and diagnosis of malaria. We previously developed a DNA aptamer that recognises the Plasmodium falciparum lactate dehydrogenase (PfLDH) enzyme with high sensitivity and specificity. The aptamer was integrated into an Aptamer-Tethered E...